AB133521

Anti-HOXB4 antibody [EPR1917]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(8 Publications)

Rabbit Recombinant Monoclonal HOXB4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

HOX2F, HOXB4, Homeobox protein Hox-B4, Homeobox protein Hox-2.6, Homeobox protein Hox-2F

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HOXB4 antibody [EPR1917] (AB133521)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HOXB4 antibody [EPR1917] (AB133521)

Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling HOXB4 with ab133521 at 1/100 dilution.

Unknown

Western blot - Anti-HOXB4 antibody [EPR1917] (AB133521)

All lanes:

Western blot - Anti-HOXB4 antibody [EPR1917] (ab133521) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

RAW 264.7 cell lysate at 10 µg

Lane 4:

PC-12 cell lysate at 10 µg

Predicted band size: 28 kDa

false

OI-RD Scanning - Anti-HOXB4 antibody [EPR1917] (AB133521)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HOXB4 antibody [EPR1917] (AB133521)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-HOXB4 antibody [EPR1917] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1917

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXB4 is a homeobox protein that functions as a transcription factor and is alternatively known as Homeobox protein Hox-B4. It plays a role in the regulation of gene expression influencing cell differentiation and development. The molecular weight of HOXB4 is approximately 28 kDa. This protein is mostly expressed in embryonic tissues and certain adult tissues including the bone marrow where it plays a part in hematopoiesis.

Biological function summary

HOXB4 is essential in maintaining stem cell populations and directing hematopoietic stem cell differentiation. It does not form part of any large complex but it interacts with DNA to control the expression of specific genes critical for cellular development. By regulating these genes HOXB4 influences the proper formation of body structures during embryogenesis.

Pathways

HOXB4 is involved in the hematopoietic cell lineage and the anterior-posterior patterning pathway. It works alongside other Hox family proteins to establish the identity of cells and tissues along the body axis. A vital interaction exists with HOXA9 within these pathways which embodies collaborative control over blood cell formation and differentiation.

Abnormalities in HOXB4 expression link to certain types of leukemia and other blood-related disorders. Overexpression can lead to increased proliferation of hematopoietic stem cells causing a potential risk in developing acute myeloid leukemia. Interaction with proteins like HOXA9 has been observed in these contexts indicating a complex network of genetic regulation disruption associated with the onset of myeloproliferative diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

See full target information HOXB4

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 15:465 PubMed39639398

2024

Using magnetic resonance relaxometry to evaluate the safety and quality of induced pluripotent stem cell-derived spinal cord progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jerome Tan,Jiahui Chen,Daniel Roxby,Wai Hon Chooi,Tan Dai Nguyen,Shi Yan Ng,Jongyoon Han,Sing Yian Chew

Stem cells translational medicine 13:387-398 PubMed38321361

2024

Label-Free and High-Throughput Removal of Residual Undifferentiated Cells From iPSC-Derived Spinal Cord Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tan Dai Nguyen,Wai Hon Chooi,Hyungkook Jeon,Jiahui Chen,Jerome Tan,Daniel N Roxby,Cheryl Yi-Pin Lee,Shi-Yan Ng,Sing Yian Chew,Jongyoon Han

iScience 26:108306 PubMed38026209

2023

Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Zheng,Zhixian Liu,Ziyu He,Jia Xu,YaNan Wang,ChenZi Gong,Ruoying Zhang,Su-Chun Zhang,Hong Chen,Wei Wang

Cell 185:3290-3306.e25 PubMed35988542

2022

Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.

Applications

Unspecified application

Species

Unspecified reactive species

Shadi Tarazi,Alejandro Aguilera-Castrejon,Carine Joubran,Nadir Ghanem,Shahd Ashouokhi,Francesco Roncato,Emilie Wildschutz,Montaser Haddad,Bernardo Oldak,Elidet Gomez-Cesar,Nir Livnat,Sergey Viukov,Dmitry Lokshtanov,Segev Naveh-Tassa,Max Rose,Suhair Hanna,Calanit Raanan,Ori Brenner,Merav Kedmi,Hadas Keren-Shaul,Tsvee Lapidot,Itay Maza,Noa Novershtern,Jacob H Hanna

Methods in molecular biology (Clifton, N.J.) 2549:359-377 PubMed33959917

2021

Generation of Cortical, Dopaminergic, Motor, and Sensory Neurons from Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shermaine Huiping Tay, Winanto,Zi Jian Khong,Yong Hui Koh,Shi Yan Ng

Stem cells (Dayton, Ohio) 38:1400-1408 PubMed32745311

2020

Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Walsh,Vincent Truong,Sushmita Nayak,Marietta Saldías Montivero,Walter C Low,Ann M Parr,James R Dutton

Theranostics 9:311-323 PubMed30809276

2019

Microhexagon gradient array directs spatial diversification of spinal motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Geok Soon Lim,Jin Hui Hor,Nicholas R Y Ho,Chi Yan Wong,Shi Yan Ng,Boon Seng Soh,Huilin Shao

Cell death & disease 9:1100 PubMed30368521

2018

Cell cycle inhibitors protect motor neurons in an organoid model of Spinal Muscular Atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hui Hor,Eunice Shi-Yi Soh,Li Yi Tan,Valerie Jing Wen Lim,Munirah Mohamad Santosa, Winanto,Beatrice Xuan Ho,Yong Fan,Boon-Seng Soh,Shi-Yan Ng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HOXB4 antibody [EPR1917]

Immunocytochemistry/ Immunofluorescence - Anti-HOXB4 antibody [EPR1917] (AB133521)

Western blot - Anti-HOXB4 antibody [EPR1917] (AB133521)

OI-RD Scanning - Anti-HOXB4 antibody [EPR1917] (AB133521)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise