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AB229345

Anti-HOXB5 antibody

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(4 Publications)

Rabbit Polyclonal HOXB5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXB5.

View Alternative Names

HOX2A, HOXB5, Homeobox protein Hox-B5, Homeobox protein HHO.C10, Homeobox protein Hox-2A, Homeobox protein Hu-1

2 Images
Immunoprecipitation - Anti-HOXB5 antibody (AB229345)
  • IP

Supplier Data

Immunoprecipitation - Anti-HOXB5 antibody (AB229345)

HOXB5 was immunoprecipitated from PC-3 (human prostate adenocarcinoma cell line) nuclear extract with 5 μg ab229345. Western blot was performed from the immunoprecipitate using ab229345. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : Control IgG IP in PC-3 nuclear extract.

Lane 2 : ab229345 IP in PC-3 nuclear extract.

All lanes:

Immunoprecipitation - Anti-HOXB5 antibody (ab229345)

Predicted band size: 29 kDa

false

Western blot - Anti-HOXB5 antibody (AB229345)
  • WB

Supplier Data

Western blot - Anti-HOXB5 antibody (AB229345)

12 % SDS-PAGE gel.

All lanes:

Western blot - Anti-HOXB5 antibody (ab229345) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HepG2 nuclear extract at 30 µg

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human HOXB5. The exact immunogen used to generate this antibody is proprietary information.

P09067

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXB5 also known as Homeobox B5 acts as a transcription factor within the body. This gene-encoded protein weighs approximately 29 kDa and plays an essential role in the development and differentiation of various tissues. It exhibits expression in a range of tissues including the blood and nervous systems. By binding to DNA HOXB5 assists in regulating the expression of genes important for body patterning and cellular function.
Biological function summary

The HOXB5 protein influences early development processes and organogenesis. It contributes significantly to the establishment of anterior-posterior body axis during embryonic development. HOXB5 does not form part of a larger complex but interacts with other transcription factors and DNA regions to carry out its functions. Its regulation of gene expression has implications for cellular differentiation and tissue specification.

Pathways

HOXB5 plays a significant role in the Wnt signaling and retinoic acid signaling pathways. These pathways govern processes such as cell growth differentiation and axonal development. HOXB5 interacts with other proteins like β-catenin in the Wnt pathway influencing developmental processes through these interactions. Its activity contributes to the orchestration of developmental signals that ensure proper morphological outcomes.

Mutations or dysregulation of HOXB5 have connections to certain types of cancer and developmental disorders such as cervical cancer and congenital defects. In these contexts HOXB5 often acts together with proteins like retinoic acid receptors highlighting its role in aberrant cellular proliferation and incorrect tissue formation. Understanding HOXB5’s involvement in these diseases highlights its importance as a potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
See full target information HOXB5
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology research and practice 2025:5790162 PubMed40463822

2025

Changrun Formula Relieves Functional Constipation by Improving Intestinal Motility in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuping Xiao,Yanqiu Hong,Xuesi Geng

Experimental and therapeutic medicine 24:585 PubMed35949323

2022

HOXB5-activated ANGPT2 promotes the proliferation, migration, invasion and angiogenic effect of esophageal cancer cells via activating ERK/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Shanshan Gao

Bioengineered 13:5126-5140 PubMed35170372

2022

Circ_0020123 enhances the cisplatin resistance in non-small cell lung cancer cells partly by sponging miR-140-3p to regulate homeobox B5 (HOXB5).

Applications

Unspecified application

Species

Unspecified reactive species

Dong Wei,Jing Zeng,Feng Rong,Yasheng Xu,Rong Wei,Can Zou

Theranostics 11:2612-2633 PubMed33456563

2021

CXCL12-mediated HOXB5 overexpression facilitates Colorectal Cancer metastasis through transactivating CXCR4 and ITGB3.

Applications

Unspecified application

Species

Unspecified reactive species

Weibo Feng,Wenjie Huang,Jie Chen,Chenyang Qiao,Danfei Liu,Xiaoyu Ji,Meng Xie,Tongyue Zhang,Yijun Wang,Mengyu Sun,Dean Tian,Daiming Fan,Yongzhan Nie,Kaichun Wu,Limin Xia
View all publications
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