Rabbit Polyclonal HOXB5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXB5.
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Publications
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- Experimental and therapeutic medicine 24:5852022HOXB5-activated ANGPT2 promotes the proliferation, migration, invasion and angiogenic effect of esophageal cancer cells via activating ERK/AKT signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Li,Shanshan GaoPubMed 35949323
- Bioengineered 13:5126-51402022Circ_0020123 enhances the cisplatin resistance in non-small cell lung cancer cells partly by sponging miR-140-3p to regulate homeobox B5 (HOXB5).Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Wei,Jing Zeng,Feng Rong,Yasheng Xu,Rong Wei,Can ZouPubMed 35170372
- Theranostics 11:2612-26332021CXCL12-mediated HOXB5 overexpression facilitates Colorectal Cancer metastasis through transactivating CXCR4 and ITGB3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeibo Feng et. al.PubMed 33456563
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HOXB5. The exact immunogen used to generate this antibody is proprietary information. Database link P09067
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Alternative names
HOX2A, HOXB5, Homeobox protein Hox-B5, Homeobox protein HHO.C10, Homeobox protein Hox-2A, Homeobox protein Hu-1
Recommended products
Rabbit Polyclonal HOXB5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXB5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HOXB5. The exact immunogen used to generate this antibody is proprietary information. Database link P09067
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HOXB5 also known as Homeobox B5 acts as a transcription factor within the body. This gene-encoded protein weighs approximately 29 kDa and plays an essential role in the development and differentiation of various tissues. It exhibits expression in a range of tissues including the blood and nervous systems. By binding to DNA HOXB5 assists in regulating the expression of genes important for body patterning and cellular function.
- Biological function summary
The HOXB5 protein influences early development processes and organogenesis. It contributes significantly to the establishment of anterior-posterior body axis during embryonic development. HOXB5 does not form part of a larger complex but interacts with other transcription factors and DNA regions to carry out its functions. Its regulation of gene expression has implications for cellular differentiation and tissue specification.
- Pathways
HOXB5 plays a significant role in the Wnt signaling and retinoic acid signaling pathways. These pathways govern processes such as cell growth differentiation and axonal development. HOXB5 interacts with other proteins like β-catenin in the Wnt pathway influencing developmental processes through these interactions. Its activity contributes to the orchestration of developmental signals that ensure proper morphological outcomes.
- Associated diseases and disorders
Mutations or dysregulation of HOXB5 have connections to certain types of cancer and developmental disorders such as cervical cancer and congenital defects. In these contexts HOXB5 often acts together with proteins like retinoic acid receptors highlighting its role in aberrant cellular proliferation and incorrect tissue formation. Understanding HOXB5’s involvement in these diseases highlights its importance as a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-HOXB5 antibody (ab229345)
12 % SDS-PAGE gel.
All lanes: Western blot - Anti-HOXB5 antibody (ab229345) at 1/500 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Lane 2: HepG2 nuclear extract at 30 µg
Predicted band size: 29 kDa
Immunoprecipitation - Anti-HOXB5 antibody (ab229345)
HOXB5 was immunoprecipitated from PC-3 (human prostate adenocarcinoma cell line) nuclear extract with 5 μg ab229345. Western blot was performed from the immunoprecipitate using ab229345. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in PC-3 nuclear extract.
Lane 2: ab229345 IP in PC-3 nuclear extract.
All lanes: Immunoprecipitation - Anti-HOXB5 antibody (ab229345)
Predicted band size: 29 kDa
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