AB109375

Anti-HOXB5 antibody [EP2827]

Name: Anti-HOXB5 antibody [EP2827] (ab109375) | Abcam
Brand: Abcam Limited
SKU: AB109375
Price: 240 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
What is this?

(1 Review)

(7 Publications)

Rabbit Monoclonal HOXB5 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

HOX2A, HOXB5, Homeobox protein Hox-B5, Homeobox protein HHO.C10, Homeobox protein Hox-2A, Homeobox protein Hu-1

2 Images

Unknown

Western blot - Anti-HOXB5 antibody [EP2827] (AB109375)

All lanes:

Western blot - Anti-HOXB5 antibody [EP2827] (ab109375) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg/mL

Lane 2:

Human fetal kidney lysate at 10 µg/mL

Predicted band size: 29 kDa

false

Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] (AB109375)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] (AB109375)

ab109375 staining the HOXB5 in U-87 MG (Human glioblastoma-astrocytoma epithelial cell) by intracellular flow cytometry. The sample was incubated with the primary antibody at 1/80 (Pink) for 30 minutes at 4°C. A FITC-conjugated Goat anti-rabbit IgG (1/150) was used as the secondary antibody.

Rabbit monoclonal IgG was used as an Isotype control (Green)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2827

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXB5 also known as Homeobox B5 acts as a transcription factor within the body. This gene-encoded protein weighs approximately 29 kDa and plays an essential role in the development and differentiation of various tissues. It exhibits expression in a range of tissues including the blood and nervous systems. By binding to DNA HOXB5 assists in regulating the expression of genes important for body patterning and cellular function.

Biological function summary

The HOXB5 protein influences early development processes and organogenesis. It contributes significantly to the establishment of anterior-posterior body axis during embryonic development. HOXB5 does not form part of a larger complex but interacts with other transcription factors and DNA regions to carry out its functions. Its regulation of gene expression has implications for cellular differentiation and tissue specification.

Pathways

HOXB5 plays a significant role in the Wnt signaling and retinoic acid signaling pathways. These pathways govern processes such as cell growth differentiation and axonal development. HOXB5 interacts with other proteins like β-catenin in the Wnt pathway influencing developmental processes through these interactions. Its activity contributes to the orchestration of developmental signals that ensure proper morphological outcomes.

Mutations or dysregulation of HOXB5 have connections to certain types of cancer and developmental disorders such as cervical cancer and congenital defects. In these contexts HOXB5 often acts together with proteins like retinoic acid receptors highlighting its role in aberrant cellular proliferation and incorrect tissue formation. Understanding HOXB5’s involvement in these diseases highlights its importance as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

See full target information HOXB5

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0127625 PubMed40874743

2025

Camptothecin, a topoisomerase I inhibitor, impedes productive herpes simplex virus type 1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph R Heath,Alison E Lloyd,Raegen M Kulinski,Daniel P Fromuth,Jill A Dembowski

Oncology research 29:411-423 PubMed37304647

2023

LncRNA PRRT3-AS1 exerts oncogenic effects on nonsmall cell lung cancer by targeting microRNA-507/homeobox B5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhou,Jianyang Xu,Lingwei Wang,Jianxin Li

Experimental and therapeutic medicine 24:585 PubMed35949323

2022

HOXB5-activated ANGPT2 promotes the proliferation, migration, invasion and angiogenic effect of esophageal cancer cells via activating ERK/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Shanshan Gao

Frontiers in genetics 12:678368 PubMed34421991

2021

The Prognostic Value and Function of HOXB5 in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Chen,Yi Qu,Pengjie Yue,Xiaojing Yan

Journal of experimental & clinical cancer research : CR 40:134 PubMed33858489

2021

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junshuang Zhao,Yang Jiang,Haiying Zhang,Jinpeng Zhou,Lian Chen,Hao Li,Jinkun Xu,Guoqing Zhang,Zhitao Jing

Journal of experimental & clinical cancer research 39:169 PubMed32847606

2020

CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Lili Yin,Xiaofei Sun

International journal of molecular medicine 46:119-130 PubMed32319550

2020

Long non‑coding RNA PRNCR1 exerts oncogenic effects in tongue squamous cell carcinoma in vitro and in vivo by sponging microRNA‑944 and thereby increasing HOXB5 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Lin,Yanan Zou,Ruijing Li,Daofeng Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HOXB5 antibody [EP2827]

Western blot - Anti-HOXB5 antibody [EP2827] (AB109375)

Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] (AB109375)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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