AB284782

Anti-HOXB5 antibody [EP2827] - BSA and Azide free

RabMAb
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Rabbit Monoclonal HOXB5 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

HOX2A, HOXB5, Homeobox protein Hox-B5, Homeobox protein HHO.C10, Homeobox protein Hox-2A, Homeobox protein Hu-1

2 Images

Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782)

This data was developed using ab109375, the same antibody clone in a different buffer formulation.

ab109375 staining the HOXB5 in U-87 MG (Human glioblastoma-astrocytoma epithelial cell) by intracellular flow cytometry. The sample was incubated with the primary antibody at 1/80 (Pink) for 30 minutes at 4°C. A FITC-conjugated Goat anti-rabbit IgG (1/150) was used as the secondary antibody.

Rabbit monoclonal IgG was used as an Isotype control (Green)

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Western blot - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782)

This data was developed using ab109375, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HOXB5 antibody [EP2827] (<a href='/en-us/products/primary-antibodies/hoxb5-antibody-ep2827-ab109375'>ab109375</a>) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg/mL

Lane 2:

Human fetal kidney lysate at 10 µg/mL

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2827

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

ab284782 is the carrier-free version of ab109375

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXB5 also known as Homeobox B5 acts as a transcription factor within the body. This gene-encoded protein weighs approximately 29 kDa and plays an essential role in the development and differentiation of various tissues. It exhibits expression in a range of tissues including the blood and nervous systems. By binding to DNA HOXB5 assists in regulating the expression of genes important for body patterning and cellular function.

Biological function summary

The HOXB5 protein influences early development processes and organogenesis. It contributes significantly to the establishment of anterior-posterior body axis during embryonic development. HOXB5 does not form part of a larger complex but interacts with other transcription factors and DNA regions to carry out its functions. Its regulation of gene expression has implications for cellular differentiation and tissue specification.

Pathways

HOXB5 plays a significant role in the Wnt signaling and retinoic acid signaling pathways. These pathways govern processes such as cell growth differentiation and axonal development. HOXB5 interacts with other proteins like β-catenin in the Wnt pathway influencing developmental processes through these interactions. Its activity contributes to the orchestration of developmental signals that ensure proper morphological outcomes.

Mutations or dysregulation of HOXB5 have connections to certain types of cancer and developmental disorders such as cervical cancer and congenital defects. In these contexts HOXB5 often acts together with proteins like retinoic acid receptors highlighting its role in aberrant cellular proliferation and incorrect tissue formation. Understanding HOXB5’s involvement in these diseases highlights its importance as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

See full target information HOXB5

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HOXB5 antibody [EP2827] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782)

Western blot - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise