Rabbit Monoclonal HOXB5 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782), expandable thumbnail](https://content.abcam.com/products/images/hoxb5-antibody-ep2827-bsa-and-azide-free-ab284782--western-blot-img124982.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782)")
![Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782), expandable thumbnail](https://content.abcam.com/products/images/hoxb5-antibody-ep2827-bsa-and-azide-free-ab284782--flow-cytometry-intracellular-img62947.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (AB284782)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target data
Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Alternative names
HOX2A, HOXB5, Homeobox protein Hox-B5, Homeobox protein HHO.C10, Homeobox protein Hox-2A, Homeobox protein Hu-1
Recommended products
Rabbit Monoclonal HOXB5 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP2827
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284782 is the carrier-free version of Anti-HOXB5 antibody [EP2827] ab109375
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HOXB5 also known as Homeobox B5 acts as a transcription factor within the body. This gene-encoded protein weighs approximately 29 kDa and plays an essential role in the development and differentiation of various tissues. It exhibits expression in a range of tissues including the blood and nervous systems. By binding to DNA HOXB5 assists in regulating the expression of genes important for body patterning and cellular function.
- Biological function summary
The HOXB5 protein influences early development processes and organogenesis. It contributes significantly to the establishment of anterior-posterior body axis during embryonic development. HOXB5 does not form part of a larger complex but interacts with other transcription factors and DNA regions to carry out its functions. Its regulation of gene expression has implications for cellular differentiation and tissue specification.
- Pathways
HOXB5 plays a significant role in the Wnt signaling and retinoic acid signaling pathways. These pathways govern processes such as cell growth differentiation and axonal development. HOXB5 interacts with other proteins like β-catenin in the Wnt pathway influencing developmental processes through these interactions. Its activity contributes to the orchestration of developmental signals that ensure proper morphological outcomes.
- Associated diseases and disorders
Mutations or dysregulation of HOXB5 have connections to certain types of cancer and developmental disorders such as cervical cancer and congenital defects. In these contexts HOXB5 often acts together with proteins like retinoic acid receptors highlighting its role in aberrant cellular proliferation and incorrect tissue formation. Understanding HOXB5’s involvement in these diseases highlights its importance as a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (ab284782), expandable thumbnail](https://content.abcam.com/products/images/hoxb5-antibody-ep2827-bsa-and-azide-free-ab284782--western-blot-img124982.jpg "Western blot - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (ab284782)")
Western blot - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (ab284782)
This data was developed using Anti-HOXB5 antibody [EP2827] ab109375, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HOXB5 antibody [EP2827] (Anti-HOXB5 antibody [EP2827] ab109375) at 1/1000 dilution
Lane 1: Human fetal lung lysate at 10 µg/mL
Lane 2: Human fetal kidney lysate at 10 µg/mL
Predicted band size: 29 kDa
![Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (ab284782), expandable thumbnail](https://content.abcam.com/products/images/hoxb5-antibody-ep2827-bsa-and-azide-free-ab284782--flow-cytometry-intracellular-img62947.jpg "Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (ab284782)")
Flow Cytometry (Intracellular) - Anti-HOXB5 antibody [EP2827] - BSA and Azide free (ab284782)
This data was developed using Anti-HOXB5 antibody [EP2827] ab109375, the same antibody clone in a different buffer formulation.
Anti-HOXB5 antibody [EP2827] ab109375 staining the HOXB5 in U-87 MG (Human glioblastoma-astrocytoma epithelial cell) by intracellular flow cytometry. The sample was incubated with the primary antibody at 1/80 (Pink) for 30 minutes at 4°C. A FITC-conjugated Goat anti-rabbit IgG (1/150) was used as the secondary antibody.
Rabbit monoclonal IgG was used as an Isotype control (Green)
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