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AB248623

Anti-HOXB9 antibody [EPR6950] - BSA and Azide free

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Rabbit Recombinant Monoclonal HOXB9 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

HOX2E, HOXB9, Homeobox protein Hox-B9, Homeobox protein Hox-2.5, Homeobox protein Hox-2E

2 Images
Western blot - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (AB248623)
  • WB

Unknown

Western blot - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (AB248623)

This data was developed using ab133701, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HOXB9 antibody [EPR6950] (<a href='/en-us/products/primary-antibodies/hoxb9-antibody-epr6950-ab133701'>ab133701</a>) at 1/1000 dilution

All lanes:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

OI-RD Scanning - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (AB248623)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (AB248623)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6950

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248623 is the carrier-free version of ab133701.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HOXB9 protein also known as homeobox B9 acts as a transcription factor. This protein has a role in regulating gene expression during development. HOXB9 weighs approximately 27 kDa and it finds expression mainly in the lungs digestive system and hematopoietic cells. As part of the homeobox gene family HOXB9 contributes to the proper development and differentiation of tissues.
Biological function summary

HOXB9 helps regulate the growth and development of tissues and organs. It does not function alone but is part of a larger group of homeobox genes all of which work together for normal morphological development. This protein provides specificity in binding DNA sequences which in turn controls the expression of genes necessary for organogenesis.

Pathways

HOXB9 plays a role in the Wnt signaling pathway and the hedgehog signaling pathway. These pathways are essential for proper embryonic development. Within these pathways HOXB9 interacts with other proteins like β-catenin and Gli proteins supporting the regulation of cellular proliferation and differentiation.

HOXB9 shows a significant relation to breast cancer and lung adenocarcinoma. In these cancers HOXB9 expression levels often increase contributing to tumor progression. This protein interacts with others involved in cancer pathways such as VEGF and MMPs promoting angiogenesis and metastasis. Researchers closely study HOXB9 as a potential target for therapeutic intervention in such malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
See full target information HOXB9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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