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Rabbit Recombinant Monoclonal HOXB9 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (AB248623), expandable thumbnail
  • OI-RD Scanning - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (AB248623), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-P
Human
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Alternative names

HOX2E, HOXB9, Homeobox protein Hox-B9, Homeobox protein Hox-2.5, Homeobox protein Hox-2E

Recommended products

Rabbit Recombinant Monoclonal HOXB9 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6950
Purification technique
Affinity purification
Dissociation constant
1.01 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248623 is the carrier-free version of Anti-HOXB9 antibody [EPR6950] ab133701.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The HOXB9 protein also known as homeobox B9 acts as a transcription factor. This protein has a role in regulating gene expression during development. HOXB9 weighs approximately 27 kDa and it finds expression mainly in the lungs digestive system and hematopoietic cells. As part of the homeobox gene family HOXB9 contributes to the proper development and differentiation of tissues.

Biological function summary

HOXB9 helps regulate the growth and development of tissues and organs. It does not function alone but is part of a larger group of homeobox genes all of which work together for normal morphological development. This protein provides specificity in binding DNA sequences which in turn controls the expression of genes necessary for organogenesis.

Pathways

HOXB9 plays a role in the Wnt signaling pathway and the hedgehog signaling pathway. These pathways are essential for proper embryonic development. Within these pathways HOXB9 interacts with other proteins like β-catenin and Gli proteins supporting the regulation of cellular proliferation and differentiation.

Associated diseases and disorders

HOXB9 shows a significant relation to breast cancer and lung adenocarcinoma. In these cancers HOXB9 expression levels often increase contributing to tumor progression. This protein interacts with others involved in cancer pathways such as VEGF and MMPs promoting angiogenesis and metastasis. Researchers closely study HOXB9 as a potential target for therapeutic intervention in such malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (ab248623), expandable thumbnail

    Western blot - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (ab248623)

    This data was developed using Anti-HOXB9 antibody [EPR6950] ab133701, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-HOXB9 antibody [EPR6950] (Anti-HOXB9 antibody [EPR6950] ab133701) at 1/1000 dilution

    All lanes: MCF7 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

  • OI-RD Scanning - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (ab248623), expandable thumbnail

    OI-RD Scanning - Anti-HOXB9 antibody [EPR6950] - BSA and Azide free (ab248623)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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