Anti-HOXC10 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXC10 antibody (AB153904)

Immunohistochemical analysis of paraffin-embedded HeLa xenograft tissue labeling HOXC10 with ab153904 at 1/500 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HOXC10 antibody (AB153904)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling HOXC10 with ab153904 at 1/2000 dilution. Alpha tubulin filaments were labeled red.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HOXC10 antibody (AB153904)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Homeobox C10 protein at nucleus using ab153904 at a 1/500 dilution (Green).
phalloidin, a cytoskeleton marker, was stained at a 1/100 dilution (Red). Scale bar = 10 µm

• WB

Unknown

Western blot - Anti-HOXC10 antibody (AB153904)

10% SDS PAGE

All lanes:

Western blot - Anti-HOXC10 antibody (ab153904) at 1/1000 dilution

All lanes:

Mouse muscle lysate at 50 µg

Predicted band size: 32 kDa,38 kDa

false

• WB

CiteAb

Western blot - Anti-HOXC10 antibody (AB153904)

Western Blotting using Anti-HOXC10 antibody, ab153904. Publication image from Dang, Y. et al., 2020, Theranostics, 32206125. Legend direct from paper.

PDPK1 and VASP are direct transcriptional targets of HOXC10. (A) Western blotting analysis of PDPK1 and VASP expression in the indicated HCC cells. (B) Real-time PCR analysis of PDPK1 and VASP expression in the indicated HCC cells. (C) HOXC10 transactivates PDPK1 and VASP promoters. The PDPK1 or VASP promoter luciferase construct was cotransfected with pCMV-HOXC10, and promoter activities were detected using a luciferase reporter assay. (D-E) Deletion and selective mutation analyses identified HOXC10-responsive regions in the (D) PDPK1 and (E) VASP promoter. Serially truncated and mutated PDPK1 or VASP promoter constructs were cotransfected with pCMV-HOXC10, and relative luciferase activities were determined. The schematic constructs are shown (left), and the bar graphs present the relative levels of luciferase activity in each of the samples (right). (F-G) ChIP assays demonstrated the direct binding of HOXC10 to the PDPK1 (F) or VASP (G) promoter in Hep3B-HOXC10 cells (left panel) and the enriched binding of endogenous HOXC10 to the PDPK1 or VASP promoter in primary HCC tissues (right panel). Real-time PCR was performed to detect the amounts of immunoprecipitated products. Hepatocytes were separated from the liver tissues of HCC patients and healthy controls (HC). The cells were crosslinked, and the chromatin was immunoprecipitated by anti-HOXC10 or control antibody. All the data are shown as the mean±s.d. * P<0.05 ** P˂0.01.

false

• WB

CiteAb

Western blot - Anti-HOXC10 antibody (AB153904)

Western Blotting using Anti-HOXC10 antibody, ab153904. Publication image from Dang, Y. et al., 2020, Theranostics, 32206125. Legend direct from paper.

HOXC10 is essential for IL-1β-mediated HCC metastasis expression. (A) Hep3B-IL-1β cells were infected with LV-shcontrol or LV-shHOXC10 by lentiviral transduction, and HOXC10 expression was examined by Western blotting. The IL-1β levels in the supernatant of the indicated cells were detected by enzyme-linked immunosorbent assay (ELISA). (B) Transwell assays showed that HOXC10 knockdown inhibited the migration and invasion abilities of Hep3B-IL-1β cells. (C-F) Knockdown of HOXC10 inhibited IL-1β-mediated HCC metastasis. (C) Bioluminescence images, metastasis incidence, and number of lung metastasis foci of the indicated groups of nude mice are shown. (D) Bioluminescence signals. (E) Overall survival. (F) Representative HE staining of lung tissues from the different groups is shown. The scale bars represent 1 mm (low magnification) and 100 µm (high magnification). (G) After Hep3B-IL-1β cells were treated with Anakinra (10 µg/ml) for 24 hr, the protein levels of HOXC10, PDPK1 and VASP were detected by Western blotting. (H) Anakinra treatment (10 µg/ml, 24 hr) significantly inhibited the migration and invasion abilities of Hep3B-IL-1β cells. (I-K) Anakinra treatment markedly inhibited IL-1β-mediated HCC metastasis. (I) Anakinra, 1 mg/kg/day, or PBS, was administered intraperitoneally for 9 weeks. starting 1 week after orthotopic implantation of the tumor. (J) The bioluminescent signals, numbers of lung metastatic foci and incidence of lung metastasis. (K) The overall survival times and representative HE staining of lung tissues from the different groups are shown. The scale bars represent 1 mm (low magnification) and 100 µm (high magnification). (L) A schematic diagram of the role of IL-1β-HOXC10 signaling in inflammation-related HCC metastasis. IL-1β-IL-1R1 signaling upregulates HOXC10 expression through the JNK/c-Jun signaling pathway. PDPK1 and VASP are direct transcriptional targets of HOXC10. HOXC10 promotes HCC invasion and metastasis by upregulating PDPK1 and VASP expression. The IL-1R1 antagonist Anakinra inhibits IL-1β-mediated HOXC10 upregulation, thereby inhibiting IL-1β-HOXC10 signaling-mediated HCC invasion and metastasis.

false

• WB

CiteAb

Western blot - Anti-HOXC10 antibody (AB153904)

Western Blotting using Anti-HOXC10 antibody, ab153904. Publication image from Dang, Y. et al., 2020, Theranostics, 32206125. Legend direct from paper.

Elevated HOXC10 expression promotes HCC invasion and metastasis and indicates a poor prognosis in human HCC. (A) Relative HOXC10 mRNA expression in 20 normal liver tissues and 90 paired HCC and adjacent nontumorous tissues (left). Relative HOXC10 mRNA expression in HCC patients with (n=48) or without (n=42) recurrence (middle). Relative HOXC10 mRNA expression in HCC patients with (n=43) or without (n=39) metastasis (right). (B) Representative images of IHC staining and IHC scores of HOXC10 in human HCC tissues from two independent cohorts of patients. The scale bars represent 250 µm (low magnification) and 50 µm (high magnification). (C) Kaplan-Meier analysis of the correlation of HOXC10 expression with recurrence and overall survival in Cohort I and Cohort II. (D) Western blotting analysis of HOXC10 expression in normal liver tissue and human HCC cell lines. (E) Western blotting analysis of HOXC10 expression in the indicated HCC cells. (F) Transwell assay analysis of the migration and invasion abilities of the indicated HCC cells. (G-J) In vivo metastasis assays. The indicated HCC cell lines were transplanted into the livers of nude mice. (G) Bioluminescent images and incidence of lung colonization. (H) Number of lung-colonizing nodules and intensity of bioluminescence signals. (I) Overall survival. (J) Representative HE staining of lung tissues from the different groups is shown (J). The scale bars represent 1 mm (low magnification) and 100 µm (high magnification). All the data are shown as the mean±s.d. * P<0.05 ** P˂0.01.

false