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Rabbit Recombinant Monoclonal HOXC10 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-HOXC10 antibody [EPR27318-83] (AB308518), expandable thumbnail
  • Western blot - Anti-HOXC10 antibody [EPR27318-83] (AB308518), expandable thumbnail
  • Western blot - Anti-HOXC10 antibody [EPR27318-83] (AB308518), expandable thumbnail
  • Western blot - Anti-HOXC10 antibody [EPR27318-83] (AB308518), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HOXC10 antibody [EPR27318-83] (AB308518), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-PIP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Alternative names

HOX3I, HOXC10, Homeobox protein Hox-C10, Homeobox protein Hox-3I

Recommended products

Rabbit Recombinant Monoclonal HOXC10 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27318-83
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HOXC10 also known as Homeobox C10 is a transcription factor that plays an important role in the regulation of gene expression. This protein weighing approximately 37 kDa belongs to the homeobox family and is involved in morphogenesis specifically in the development of the limbs and axial skeleton. Researchers have identified its expression in various tissues including the central nervous system and skeletal muscle. The presence of HOXC10 in these areas suggests its involvement in complex developmental processes and its importance in proper structural formation.

Biological function summary

HOXC10 participates in regulating cell differentiation and proliferation. It acts as a transcriptional regulator and part of the homeobox gene family which functions in determining cell fate and positional development. This factor does not function alone; it forms complexes with other proteins to exert its effects on target genes. By regulating the expression of other genes it supports the establishment of specific cellular phenotypes necessary for proper tissue and organ development.

Pathways

HOXC10 interacts with multiple signaling pathways including the Wnt and Hedgehog pathways which are critical for development and growth. In the Wnt pathway HOXC10 collaborates with other homeobox genes to influence gene expression necessary for proper cell positioning. It also relates closely with other HOX proteins such as HOXA1 and HOXD10 coordinating complex regulation processes that guide embryonic development and patterning.

Associated diseases and disorders

HOXC10 has connections to several pathological conditions including cancers and neurological disorders. Aberrant expression of HOXC10 has been linked with breast cancer where it is believed to promote cancer cell proliferation and survival. Additionally changes in HOXC10 expression are associated with developmental disorders involving skeletal and neural defects. Proteins like HOXB7 and HOXD13 interact with HOXC10 showing their involvement in the regulation of pathways that when disrupted may lead to disease states. Understanding its role better could offer insights into targeted therapies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-HOXC10 antibody [EPR27318-83] (ab308518), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HOXC10 antibody [EPR27318-83] (ab308518)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling HOXC10 with ab308518 at 1/50 (9.02 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line.Negative control: HepG2.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518), expandable thumbnail

    Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 3 seconds

    All lanes: Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell)cells transfected with an empty vector containing a his tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human HOXC10 expression vector containing a his tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa

    Observed band size: 40 kDa

    Exposure time: 3s

  • Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518), expandable thumbnail

    Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. Additional bands may be due to post-translational modification by ubiquitination (PMID:12853486). 

    The Lane 1 was freshly made and used for Western blotting immediately to minimize protein degradation.

    The lane 2 was developed using a high sensitivity ECL substrate.

    Exposure time: Lane 1:114 seconds, Lane 2:125 seconds

    All lanes: Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518) at 1/1000 dilution

    Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

    Lane 2: Human breast tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518), expandable thumbnail

    Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: K562, HL-60 (PMID: 7527557), HepG2

    Additional bands may be due to post-translational modification by ubiquitination (PMID:12853486).

    This blot was developed using a higher sensitivity ECL substrate.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 169 seconds

    All lanes: Western blot - Anti-HOXC10 antibody [EPR27318-83] (ab308518) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 5: HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa

    Observed band size: 40 kDa

    Exposure time: 169s

  • Flow Cytometry (Intracellular) - Anti-HOXC10 antibody [EPR27318-83] (ab308518), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HOXC10 antibody [EPR27318-83] (ab308518)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labelling HOXC10 with ab308518 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: HepG2.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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