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AB151575

Anti-HoxC6 antibody [EPR7922(2)]

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(8 Publications)

Rabbit Recombinant Monoclonal HoxC6 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications.

View Alternative Names

HOX3C, HOXC6, Homeobox protein Hox-C6, Homeobox protein CP25, Homeobox protein HHO.C8, Homeobox protein Hox-3C

3 Images
Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] (AB151575)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] (AB151575)

Intracellular flow cytometric analysis of permeabilized MCF7 cells labeling HoxC6 with ab151575 at 1/100 dilution (red), compared to a Rabbit IgG negative control antibody (green).

Western blot - Anti-HoxC6 antibody [EPR7922(2)] (AB151575)
  • WB

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Western blot - Anti-HoxC6 antibody [EPR7922(2)] (AB151575)

All lanes:

Western blot - Anti-HoxC6 antibody [EPR7922(2)] (ab151575) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

PC3 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 26 kDa

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Western blot - Anti-HoxC6 antibody [EPR7922(2)] (AB151575)
  • WB

CiteAb

Western blot - Anti-HoxC6 antibody [EPR7922(2)] (AB151575)

HoxC6 western blot using anti-HoxC6 antibody [EPR7922(2)] ab151575. Publication image and figure legend from Zhang, F., Ren, C. C., et al., 2018, Cancer Cell Int, PubMed 30559605.

ab151575 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab151575 please see the product overview.

HOXC6 gene silencing inhibits EMT and proliferative ability of CC cells by suppressing the activation of the TGF-β/smad signaling pathway. a Protein expression of HOXC6, TGF-β1, TGF-β RII, smad4, smad7, E-cadherin, N-cadherin, and Vimentin determined by western blot analysis; b cell proliferation in each group after transduction; c cell cycle in each group after transduction; d cell apoptosis in each group after transduction; *p < 0.05, compared with the HOXC6-siRNA + HA-NC group; #p < 0.05, compared with the HA-HOXC6 + si-NC group; HOXC6, homeobox C6; EMT, epithelial-mesenchymal transition; CC cervical carcinoma, TGF-β transforming growth factor-β, smad solvated metal atom dispersion, NC negative control

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  • Carrier free

    Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7922(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB140611

Anti-HSPC150/UBE2T antibody [EPR9446]

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Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (AB140611)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HoxC6 also known as Homeobox C6 is a transcription factor that guides the formation of body structure during early development. The protein weighs approximately 25 kDa and plays an important role in sequencing and pattern formation in the embryonic development stages. It is expressed mainly in the central nervous system and developing tissues but also shows functionality in adult tissues. The activity of HoxC6 affects cellular differentiation and proliferation by regulating specific gene expression in these regions.
Biological function summary

The protein binds to DNA at specific sequences to activate or repress target gene transcription. This can affect cell fate and organ morphogenesis. HoxC6 associates with other Hox proteins and cofactors to form complexes that enhance or modify its regulatory functions. These interactions with cofactor proteins further strengthen its impact on developmental processes ensuring accurate tissue and organ formation.

Pathways

HoxC6 functions within pivotal developmental pathways such as the Hox gene regulatory network. This network synchronizes with signaling pathways like the Wnt and Notch controlling cell proliferation and differentiation. The collaboration with these pathways allows HoxC6 to manage gene cascades that maintain the correct body patterning. HoxB5 a related protein in the same pathway often interacts or shares similar roles coordinating to maintain orderly tissue patterning.

Malfunction or mis-expression of HoxC6 links to certain cancers including leukemia and breast cancer. The protein's deregulation can lead to abnormal cell growth and differentiation. It works alongside proteins like Myc which also plays a central role in cancer to influence oncogenesis. Studies continue to explore HoxC6's involvement in these diseases considering its role in normal cellular development and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
See full target information HOXC6
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 43:101918 PubMed38412662

2024

Comprehensive analysis and experimental verification of the mechanism of action of T cell-mediated tumor-killing related genes in Colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Zhengfang Wang,Qin Zhu,Shiqi Ren,Yanhua Xu,Guangzhou Wang,Lin Zhou

Cancer cell international 22:170 PubMed35488304

2022

HOXC6 impacts epithelial-mesenchymal transition and the immune microenvironment through gene transcription in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Huang,Zhengyuan Huo,Jiantong Jiao,Wei Ji,Jin Huang,Zheng Bian,Bin Xu,Junfei Shao,Jun Sun

Journal of cellular physiology 236:1913-1925 PubMed32740941

2020

HomeoboxC6 affects the apoptosis of human vascular endothelial cells and is involved in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangshu Long,Ganhua You,Qiang Wu,Yu Zhou,Yan Xiao,Fuxun Yu,Shiyan Deng,Rui Mo,Fang Song,Jing Huang,Maobo Tian

Cell proliferation 53:e12823 PubMed32515533

2020

LncRNA BBOX1-AS1 upregulates HOXC6 expression through miR-361-3p and HuR to drive cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Xu,Baohua Yang,Lifeng Wang,Yunheng Zhu,Xiuxiang Zhu,Ziyin Xia,Zhen Zhao,Ling Xu

Cancer science 110:1735-1745 PubMed30844117

2019

Targeting HOX/PBX dimer formation as a potential therapeutic option in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lu-Yan Shen,Ting Zhou,Ya-Bing Du,Qi Shi,Ke-Neng Chen

Cancer cell international 18:204 PubMed30559605

2018

HOXC6 gene silencing inhibits epithelial-mesenchymal transition and cell viability through the TGF-β/smad signaling pathway in cervical carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhang,Chen-Chen Ren,Ling Liu,Yan-Nan Chen,Li Yang,Xiao-An Zhang

Pathology, research and practice 214:1818-1824 PubMed30228024

2018

Knockdown of HOXC6 inhibits glioma cell proliferation and induces cell cycle arrest by targeting WIF-1 in vitro and vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Teng-Feng Yan,Miao-Jing Wu,Bing Xiao,Qing Hu,Yang-Hua Fan,Xin-Gen Zhu

Oncology letters 14:4835-4840 PubMed29085488

2017

Increased HOXC6 expression predicts chemotherapy sensitivity in patients with esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lu-Yan Shen,Meng-Ying Fan,Bin Dong,Wan-Pu Yan,Ke-Neng Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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