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AB249014

Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal HoxC6 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

HOX3C, HOXC6, Homeobox protein Hox-C6, Homeobox protein CP25, Homeobox protein HHO.C8, Homeobox protein Hox-3C

2 Images
Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)

This data was developed using ab151575, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized MCF7 cells labeling HoxC6 with ab151575 at 1/100 dilution (red), compared to a Rabbit IgG negative control antibody (green).

Western blot - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)
  • WB

Unknown

Western blot - Anti-HoxC6 antibody [EPR7922(2)] - BSA and Azide free (AB249014)

This data was developed using ab151575, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HoxC6 antibody [EPR7922(2)] (<a href='/en-us/products/primary-antibodies/hoxc6-antibody-epr79222-ab151575'>ab151575</a>) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

PC3 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7922(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249014 is the carrier-free version of ab151575.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HoxC6 also known as Homeobox C6 is a transcription factor that guides the formation of body structure during early development. The protein weighs approximately 25 kDa and plays an important role in sequencing and pattern formation in the embryonic development stages. It is expressed mainly in the central nervous system and developing tissues but also shows functionality in adult tissues. The activity of HoxC6 affects cellular differentiation and proliferation by regulating specific gene expression in these regions.
Biological function summary

The protein binds to DNA at specific sequences to activate or repress target gene transcription. This can affect cell fate and organ morphogenesis. HoxC6 associates with other Hox proteins and cofactors to form complexes that enhance or modify its regulatory functions. These interactions with cofactor proteins further strengthen its impact on developmental processes ensuring accurate tissue and organ formation.

Pathways

HoxC6 functions within pivotal developmental pathways such as the Hox gene regulatory network. This network synchronizes with signaling pathways like the Wnt and Notch controlling cell proliferation and differentiation. The collaboration with these pathways allows HoxC6 to manage gene cascades that maintain the correct body patterning. HoxB5 a related protein in the same pathway often interacts or shares similar roles coordinating to maintain orderly tissue patterning.

Malfunction or mis-expression of HoxC6 links to certain cancers including leukemia and breast cancer. The protein's deregulation can lead to abnormal cell growth and differentiation. It works alongside proteins like Myc which also plays a central role in cancer to influence oncogenesis. Studies continue to explore HoxC6's involvement in these diseases considering its role in normal cellular development and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
See full target information HOXC6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com