AB50839

Anti-HOXC9 antibody [HOXCA6E6]

Name: Anti-HOXC9 antibody [HOXCA6E6] Mouse monoclonal (ab50839) | Abcam
Brand: Abcam Limited
SKU: AB50839
Price: 585 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(18 Publications)

Mouse Monoclonal HOXC9 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXC9.

View Alternative Names

HOX3B, HOXC9, Homeobox protein Hox-C9, Homeobox protein Hox-3B

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-HOXC9 antibody [HOXCA6E6] (AB50839)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HOXC9 antibody [HOXCA6E6] (AB50839)

ab50839 at a 1/5 dilution staining HOXC9 in Hela cells using Alexa Fluor^® 488 Goat Anti-mouse IgG at a 1/200 dilution.

Unknown

Western blot - Anti-HOXC9 antibody [HOXCA6E6] (AB50839)

All lanes:

Western blot - Anti-HOXC9 antibody [HOXCA6E6] (ab50839) at 1/100 dilution

All lanes:

HT-1080 whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HOXCA6E6

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HOXC9. The exact immunogen used to generate this antibody is proprietary information.

P31274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through a 0.22µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXC9 also known as Homeobox C9 is a transcription factor that belongs to the homeobox family specifically the HOX gene cluster. It regulates gene expression by binding to specific DNA sequences. The protein has a molecular mass of about 28 kDa. HOXC9 is expressed in various tissues including the nervous system particularly during embryonic development. It plays an important role in patterning the anterior-posterior axis and the development of specific body structures.

Biological function summary

Genes that HOXC9 regulates play key roles in developmental processes such as neural differentiation and limb formation. HOXC9 might operate as part of a larger transcriptional complex that involves other HOX proteins allowing precise control over developmental gene expression. The protein ensures that cells at different developmental stages respond correctly to morphogenetic signals facilitating proper organismal formation.

Pathways

HOXC9 actively participates in the retinoic acid signaling pathway important for neurodevelopmental regulation. This pathway requires a balance between HOXC9 and Receptor Interacting Protein 1 (RIP1) for correct gene expression. Another critical pathway is the Wnt signaling pathway where HOXC9 interacts with components such as beta-catenin to further modulate gene transcription related to cell fate decisions.

Research links HOXC9 dysregulation to neurodevelopmental disorders and certain cancers like leukemia. Abnormal HOXC9 expression disrupts regular neural development and contributes to malignancy. Its interaction with proteins including members of the Polycomb group like Bmi1 influences these pathologies. Understanding HOXC9's involvement helps in devising therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

See full target information HOXC9

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112919 PubMed41054515

2025

Integrative multi-omics analysis decodes HOXC9-driven malignant transformation and metastasis in OSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyu Xu,Zewen Sun,Dandan Wang,Guoxin Li,Wei Feng,Chunyan Qiao,Ce Shi,Qilin Liu,Peng Chen,Zhengwen An

The EMBO journal 43:6310-6335 PubMed39528654

2024

METTL3/MYCN cooperation drives neural crest differentiation and provides therapeutic vulnerability in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ketan Thombare,Roshan Vaid,Perla Pucci,Kristina Ihrmark Lundberg,Ritish Ayyalusamy,Mohammad Hassan Baig,Akram Mendez,Rebeca Burgos-Panadero,Stefanie Höppner,Christoph Bartenhagen,Daniel Sjövall,Aqsa Ali Rehan,Sagar Dattatraya Nale,Anna Djos,Tommy Martinsson,Pekka Jaako,Jae-June Dong,Per Kogner,John Inge Johnsen,Matthias Fischer,Suzanne D Turner,Tanmoy Mondal

Cell stem cell 31:1113-1126.e6 PubMed38981471

2024

A human pluripotent stem cell-based somitogenesis model using microfluidics.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Liu,Yung Su Kim,Xufeng Xue,Yuchuan Miao,Norio Kobayashi,Shiyu Sun,Robin Zhexuan Yan,Qiong Yang,Olivier Pourquié,Jianping Fu

Cell reports methods 4:100777 PubMed38744289

2024

Effective cryopreservation of human brain tissue and neural organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Xue,Huijuan Li,Jinhong Xu,Xiao Yu,Linlin Liu,Huihui Liu,Rui Zhao,Zhicheng Shao

Nature communications 15:3745 PubMed38702304

2024

A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations.

Applications

Unspecified application

Species

Unspecified reactive species

Ingrid M Saldana-Guerrero,Luis F Montano-Gutierrez,Katy Boswell,Christoph Hafemeister,Evon Poon,Lisa E Shaw,Dylan Stavish,Rebecca A Lea,Sara Wernig-Zorc,Eva Bozsaky,Irfete S Fetahu,Peter Zoescher,Ulrike Pötschger,Marie Bernkopf,Andrea Wenninger-Weinzierl,Caterina Sturtzel,Celine Souilhol,Sophia Tarelli,Mohamed R Shoeb,Polyxeni Bozatzi,Magdalena Rados,Maria Guarini,Michelle C Buri,Wolfgang Weninger,Eva M Putz,Miller Huang,Ruth Ladenstein,Peter W Andrews,Ivana Barbaric,George D Cresswell,Helen E Bryant,Martin Distel,Louis Chesler,Sabine Taschner-Mandl,Matthias Farlik,Anestis Tsakiridis,Florian Halbritter

Cell stem cell 30:264-282.e9 PubMed36868194

2023

hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Fan,James Hackland,Arianna Baggiolini,Lin Y Hung,Huiyong Zhao,Paul Zumbo,Polina Oberst,Andrew P Minotti,Emiliano Hergenreder,Sarah Najjar,Zixing Huang,Nelly M Cruz,Aaron Zhong,Mega Sidharta,Ting Zhou,Elisa de Stanchina,Doron Betel,Richard M White,Michael Gershon,Kara Gross Margolis,Lorenz Studer

iScience 26:105898 PubMed36647382

2023

Generation of dorsoventral human spinal cord organoids via functionalizing composite scaffold for drug testing.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Xue,Bo Li,Huihui Liu,Yujie Xiao,Bo Li,Lei Ren,Huijuan Li,Zhicheng Shao

eLife 11: PubMed36154671

2022

Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor.

Applications

Unspecified application

Species

Unspecified reactive species

Antigoni Gogolou,Celine Souilhol,Ilaria Granata,Filip J Wymeersch,Ichcha Manipur,Matthew Wind,Thomas J R Frith,Maria Guarini,Alessandro Bertero,Christoph Bock,Florian Halbritter,Minoru Takasato,Mario R Guarracino,Anestis Tsakiridis

Current protocols 1:e244 PubMed34547185

2021

In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Matt Wind,Anestis Tsakiridis

Development (Cambridge, England) 148: PubMed33658223

2021

Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Wind,Antigoni Gogolou,Ichcha Manipur,Ilaria Granata,Larissa Butler,Peter W Andrews,Ivana Barbaric,Ke Ning,Mario R Guarracino,Marysia Placzek,Anestis Tsakiridis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HOXC9 antibody [HOXCA6E6]

Immunocytochemistry/ Immunofluorescence - Anti-HOXC9 antibody [HOXCA6E6] (AB50839)

Western blot - Anti-HOXC9 antibody [HOXCA6E6] (AB50839)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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