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AB235103

Anti-HOXD1 antibody

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Rabbit Polyclonal HOXD1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HOXD1 aa 50-150.

View Alternative Names

HOX4, HOX4G, HOXD1, Homeobox protein Hox-D1, Homeobox protein Hox-GG

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXD1 antibody (AB235103)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXD1 antibody (AB235103)

Paraffin-embedded human brain tissue stained for HOXD1 using ab235103 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

Western blot - Anti-HOXD1 antibody (AB235103)
  • WB

Supplier Data

Western blot - Anti-HOXD1 antibody (AB235103)

All lanes:

Western blot - Anti-HOXD1 antibody (ab235103) at 1/500 dilution

Lane 1:

Rat lung lysate

Lane 2:

Rat kidney lysate

Lane 3:

Mouse lung lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HOXD1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q9GZZ0

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXD1 also known as Homeobox D1 is a transcription factor that plays a role in regulating gene expression. This protein has a mass of approximately 32 kDa. HOXD1 is part of the homeobox gene family which encodes transcription factors characterized by a specific sequence that binds DNA. HOXD1 shows expression in various tissues including the central nervous system and limbs during development. Its expression patterns suggest a role in the spatial and temporal regulation of developmental processes.
Biological function summary

HOXD1 contributes to the regulation of morphogenic development influencing the body layout along the anterior-posterior axis. It is not typically shown to be part of any larger protein complexes but interacts with other transcription factors to influence gene networks. During embryonic development HOXD1 orchestrates the activation and repression of target genes guiding proper morphogenesis and differentiation. This role is significant in setting positional identity and patterning of tissues.

Pathways

The HOXD1 protein participates in key developmental signaling pathways notably the Wnt signaling pathway. Wnt signaling affects cell proliferation polarity and migration making it critical during embryogenesis. HOXD1 collaborates with other homeobox genes like HOXA and HOXB clusters to ensure precise developmental patterning. The interactions among these homeobox proteins promote coordinated regulation of gene expression necessary for orderly development.

Alterations in HOXD1 expression have been linked to developmental disorders and certain cancers. Abnormal expression of HOXD1 may contribute to conditions such as synpolydactyly which affects limb development. Studies also suggest its potential involvement in various carcinomas due to its role in cell differentiation and proliferation. HOXD1's connections with other regulatory proteins like those in homeobox family suggest its mutation or dysregulation could influence similar associated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures.
See full target information HOXD1
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Product promise

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