AB228450

Anti-HOXD8 antibody

Name: Anti-HOXD8 antibody (ab228450) | Abcam
Brand: Abcam Limited
SKU: AB228450

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(5 Publications)

Rabbit Polyclonal HOXD8 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXD8 aa 50 to C-terminus.

View Alternative Names

HOX4E, HOXD8, Homeobox protein Hox-D8, Homeobox protein Hox-4E, Homeobox protein Hox-5.4

1 Images

Supplier Data

Western blot - Anti-HOXD8 antibody (AB228450)

All lanes:

Western blot - Anti-HOXD8 antibody (ab228450) at 2 µg/mL

Lane 1:

A-375 (human malignant melanoma cell line) cell lysate

Lane 2:

NCCIT cell lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human HOXD8 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13378

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HOXD8 gene also known as Homeobox D8 encodes a homeobox protein with a molecular mass of about 30 kilodaltons. It belongs to the homeobox family of transcription factors which are key in regulating gene expression during developmental processes. HOXD8 shows expression mainly in embryonic tissues and certain adult tissues including the limbs and the central nervous system. The expression pattern of HOXD8 suggests its involvement in limb formation and neural development.

Biological function summary

HOXD8 is essential in providing positional information during morphogenesis where it operates as a transcription factor. It functions as part of the HOX complex—a group of related genes that determine the head-to-tail body plan of an organism during early embryonic development. The HOXD8 protein interacts with specific DNA sequences regulating the transcription of target genes that govern critical developmental pathways.

Pathways

HOXD8 plays a significant role in the Wnt signaling pathway which influences cell fate determination during embryogenesis. It also interacts with the hedgehog signaling pathway another critical pathway that mediates tissue patterning and growth. HOXD4 and HOXD9 other members of the HOX family often work synergistically with HOXD8 orchestrating the precise regulation of gene expression necessary for proper developmental outcomes.

Defects or misregulation of HOXD8 have been linked to congenital limb malformations and some neural developmental disorders. Alterations in the expression of HOXD8 along with HOXA13 another HOX family member can lead to conditions such as synpolydactyly or overlapping toes highlighting their coordinated roles in limb pattern formation. Research into HOXD8 contributes valuable insights into developmental dysplasia and possible therapeutic interventions for related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

See full target information HOXD8

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70016 PubMed40401147

2025

ELAVL1 promotes ferroptosis via the TRIM21/HOXD8 axis to inhibit osteogenic differentiation in congenital pseudoarticular tibia-derived mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Weihua Ye,Zheng Liu,Yaoxi Liu,Han Xiao,Qian Tan,An Yan,Guanghui Zhu

Regenerative therapy 26:42-49 PubMed38818480

2024

METTL3 promotes the osteogenic differentiation of periosteum-derived MSCs via regulation of the HOXD8/ITGA5 axis in congenital pseudarthrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Weihua Ye,Zheng Liu,Yaoxi Liu,Han Xiao,Qian Tan,An Yan,Guanghui Zhu

Regenerative therapy 21:477-485 PubMed36313394

2022

miR-30a inhibits the osteogenic differentiation of the tibia-derived MSCs in congenital pseudarthrosis via targeting HOXD8.

Applications

Unspecified application

Species

Unspecified reactive species

Weihua Ye,Yiyong Huang,Guanghui Zhu,An Yan,Yaoxi Liu,Han Xiao,Haibo Mei

Frontiers in oncology 11:683582 PubMed34222004

2021

Novel Prognostic Biomarkers in Gastric Cancer: CGB5, MKNK2, and PAPPA2.

Applications

Unspecified application

Species

Unspecified reactive species

Min Qin,Zhihai Liang,Heping Qin,Yifang Huo,Qing Wu,Huiying Yang,Guodu Tang

Cell death and differentiation 26:1700-1715 PubMed30464226

2018

Long noncoding RNA lncAIS downregulation in mesenchymal stem cells is implicated in the pathogenesis of adolescent idiopathic scoliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qianyu Zhuang,Buqing Ye,Shangyi Hui,Ying Du,Robert Chunhua Zhao,Jing Li,Zhihong Wu,Na Li,Yanbin Zhang,Hongling Li,Shengru Wang,Yang Yang,Shugang Li,Hong Zhao,Zusen Fan,Guixing Qiu,Jianguo Zhang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HOXD8 antibody

Western blot - Anti-HOXD8 antibody (AB228450)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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