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Rabbit Polyclonal HOXD8 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXD8.

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Images

Western blot - Anti-HOXD8 antibody (AB229321), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human HOXD8. The exact immunogen used to generate this antibody is proprietary information. Database link P13378

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Chimpanzee
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Chimpanzee

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Alternative names

Recommended products

Rabbit Polyclonal HOXD8 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXD8.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human HOXD8. The exact immunogen used to generate this antibody is proprietary information. Database link P13378
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The HOXD8 gene also known as Homeobox D8 encodes a homeobox protein with a molecular mass of about 30 kilodaltons. It belongs to the homeobox family of transcription factors which are key in regulating gene expression during developmental processes. HOXD8 shows expression mainly in embryonic tissues and certain adult tissues including the limbs and the central nervous system. The expression pattern of HOXD8 suggests its involvement in limb formation and neural development.

Biological function summary

HOXD8 is essential in providing positional information during morphogenesis where it operates as a transcription factor. It functions as part of the HOX complex—a group of related genes that determine the head-to-tail body plan of an organism during early embryonic development. The HOXD8 protein interacts with specific DNA sequences regulating the transcription of target genes that govern critical developmental pathways.

Pathways

HOXD8 plays a significant role in the Wnt signaling pathway which influences cell fate determination during embryogenesis. It also interacts with the hedgehog signaling pathway another critical pathway that mediates tissue patterning and growth. HOXD4 and HOXD9 other members of the HOX family often work synergistically with HOXD8 orchestrating the precise regulation of gene expression necessary for proper developmental outcomes.

Associated diseases and disorders

Defects or misregulation of HOXD8 have been linked to congenital limb malformations and some neural developmental disorders. Alterations in the expression of HOXD8 along with HOXA13 another HOX family member can lead to conditions such as synpolydactyly or overlapping toes highlighting their coordinated roles in limb pattern formation. Research into HOXD8 contributes valuable insights into developmental dysplasia and possible therapeutic interventions for related disorders.

Product promise

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1 product image

  • Western blot - Anti-HOXD8 antibody (ab229321), expandable thumbnail

    Western blot - Anti-HOXD8 antibody (ab229321)

    12% SDS-PAGE gel.

    All lanes: Western blot - Anti-HOXD8 antibody (ab229321) at 1/500 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

    Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 31 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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