AB234085

Anti-HP1 alpha antibody [3A11F8]

Name: Anti-HP1 alpha antibody [3A11F8] (ab234085) | Abcam
Brand: Abcam Limited
SKU: AB234085
Price: 525 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal HP1 alpha antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CBX5.

View Alternative Names

HP1A, CBX5, Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [3A11F8] (AB234085)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [3A11F8] (AB234085)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HP1 alpha (green) using ab234085 at 1/200 dilution in ICC/IF.

Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with AlexaFluor^®555-Phalloidin.

Flow Cytometry - Anti-HP1 alpha antibody [3A11F8] (AB234085)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-HP1 alpha antibody [3A11F8] (AB234085)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling HP1 alpha with ab234085 at 1/200 dilution (green) compared to a negative control (red).

Supplier Data

Western blot - Anti-HP1 alpha antibody [3A11F8] (AB234085)

All lanes:

Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Lane 4:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Lane 5:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 6:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-HP1 alpha antibody [3A11F8] (AB234085)

All lanes:

Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

HP1 alpha (amino acids: 1-191)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-HP1 alpha antibody [3A11F8] (AB234085)

Expected recombinant MW is 48.2 kDa

All lanes:

Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution

All lanes:

Recombinant human HP1 alpha (amino acids: 1-191) protein

Predicted band size: 22 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A11F8

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CBX5.

P45973

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HP1 alpha also known as HP1a or HP1 protein is a well-studied member of the heterochromatin protein 1 family. It plays a mechanical role in chromatin organization and gene regulation by binding to histone H3 when it's methylated at lysine 9 (H3K9me). This protein has a molecular mass of approximately 22-25 kDa. HP1 alpha is commonly expressed in the nuclei of eukaryotic cells where it localizes to heterochromatin regions and influences chromatin compaction.

Biological function summary

Heterochromatin protein 1 alpha acts as a modulator of gene silencing. It is part of a larger protein complex that contains various chromatin modifiers responsible for maintaining the silent state of certain genes. By mediating these interactions HP1 alpha helps regulate transcription replication timing and DNA repair processes. This regulation is essential for the structural maintenance of heterochromatin and plays a part in chromosomal stability.

Pathways

HP1 alpha is integral in the epigenetic regulation pathway along with other well-known proteins including SUV39H1 and SUV39H2 which are histone methyltransferases. HP1 alpha also links to the DNA damage response pathway where it recruits repair factors to maintain genomic integrity. Through these pathways HP1 alpha interacts closely with proteins like BRCA1 emphasizing its role in cellular homeostasis and response to DNA damage.

Researchers associate HP1 alpha with cancer and developmental disorders. Alterations in HP1 alpha expression or function can lead to disruptions in chromatin organization contributing to oncogenesis by affecting tumor suppressor genes. Furthermore HP1 alpha links to disorders like ICF syndrome through its relationship with the DNA methylation process where it interacts with proteins such as DNMT3B. Understanding these connections provides insights into therapeutic approaches targeting epigenetic modifications in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression (PubMed : 40440427). In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph) (PubMed : 19783980). Also recognizes and binds histone H1.4 methylated at 'Lys-26' (H1.4K26me) (PubMed : 16127177). Excluded from chromatin when histone H1.4 is Simultaneously methylated at Lys-26 (H1.4K26me) and phosphorylated at Ser-27 (H1.4S27Ph) (PubMed : 16127177). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR) (PubMed : 19783980). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1 (PubMed : 19783980, PubMed : 20231385). Required for the formation of the inner centromere (PubMed : 20231385).

See full target information CBX5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Open biology 14:240205 PubMed39657822

2024

Phosphorylation of 'SDT-like' motifs in ATRX mediates its interaction with the MRN complex and is important for ALT pathway suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Goncalves,Harshangda Bhatnagar,Siobhan Cunniffe,Richard J Gibbons,Anna M Rose,David Clynes

Nature communications 15:9171 PubMed39448645

2024

Epigenetic modulation via the C-terminal tail of H2A.Z.

Applications

Unspecified application

Species

Unspecified reactive species

László Imre,Péter Nánási,Ibtissem Benhamza,Kata Nóra Enyedi,Gábor Mocsár,Rosevalentine Bosire,Éva Hegedüs,Erfaneh Firouzi Niaki,Ágota Csóti,Zsuzsanna Darula,Éva Csősz,Szilárd Póliska,Beáta Scholtz,Gábor Mező,Zsolt Bacsó,H T Marc Timmers,Masayuki Kusakabe,Margit Balázs,György Vámosi,Juan Ausio,Peter Cheung,Katalin Tóth,David Tremethick,Masahiko Harata,Gábor Szabó

ACS nano 16:10754-10767 PubMed35803582

2022

Dynamic Heterochromatin States in Anisotropic Nuclei of Cells on Aligned Nanofibers.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Liu,Abinash Padhi,Xiaohui Zhang,Jairaj Narendran,Mark A Anastasio,Amrinder S Nain,Joseph Irudayaraj

Oncology reports 45:169-179 PubMed33416147

2021

circHIPK3 promotes proliferation and migration and invasion via regulation of miR‑637/HDAC4 signaling in osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wen,Bin Li,Ming He,Songling Teng,Yuxiu Sun,Guangbin Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HP1 alpha antibody [3A11F8]

Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [3A11F8] (AB234085)

Flow Cytometry - Anti-HP1 alpha antibody [3A11F8] (AB234085)

Western blot - Anti-HP1 alpha antibody [3A11F8] (AB234085)

Western blot - Anti-HP1 alpha antibody [3A11F8] (AB234085)

Western blot - Anti-HP1 alpha antibody [3A11F8] (AB234085)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise