Goat Polyclonal HP1 alpha antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human CBX5 aa 50-150.
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- Nature communications 14:67002023Phase separation of BuGZ regulates gut regeneration and aging through interaction with mA regulators.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiaoqiao Zhang,Kai Deng,Mengyou Liu,Shengye Yang,Wei Xu,Tong Feng,Minwen Jie,Zhiming Liu,Xiao Sheng,Haiyang Chen,Hao JiangPubMed 37872148
- BMC cancer 22:11662022The H2A.Z-KDM1A complex promotes tumorigenesis by localizing in the nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Wang,Yongqiang Qi,Fei Xiong,Da Wang,Bing Wang,Yongjun ChenPubMed 36368958
- iScience 25:1049782022Confined migration induces heterochromatin formation and alters chromatin accessibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChieh-Ren Hsia,Jawuanna McAllister,Ovais Hasan,Julius Judd,Seoyeon Lee,Richa Agrawal,Chao-Yuan Chang,Paul Soloway,Jan LammerdingPubMed 36117991
- Cells 11:2022Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKoei Okazaki,Megumi Nakano,Jun-Ichirou Ohzeki,Koichiro Otake,Kazuto Kugou,Vladimir Larionov,William C Earnshaw,Hiroshi MasumotoPubMed 35563684
- Science advances 7:eabj91112021Misexpression of genes lacking CpG islands drives degenerative changes during aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Yeong Lee,Ian Davis,Elliot H H Youth,Jonghwan Kim,Gary Churchill,James Godwin,Ron Korstanje,Samuel BeckPubMed 34910517
- Cancers 13:2021Potential Diagnostic Value of the Differential Expression of Histone H3 Variants between Low- and High-Grade Gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrati Hervás-Corpión,Andrea Gallardo-Orihuela,Inmaculada Catalina-Fernández,Irene Iglesias-Lozano,Olga Soto-Torres,Noelia Geribaldi-Doldán,Samuel Domínguez-García,Nuria Luna-García,Raquel Romero-García,Francisco Mora-López,Marianela Iriarte-Gahete,Jorge C Morales,Antonio Campos-Caro,Carmen Castro,José L Gil-Salú,Luis M ValorPubMed 34771425
- ACS chemical biology 16:1721-17362021A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2's Association with the HUSH Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJarod M Waybright,Sarah E Clinkscales,Kimberly D Barnash,Gabrielle R Budziszewski,Justin M Rectenwald,Anna M Chiarella,Jacqueline L Norris-Drouin,Stephanie H Cholensky,Kenneth H Pearce,Laura E Herring,Robert K McGinty,Nathaniel A Hathaway,Lindsey I JamesPubMed 34415726
- Molecular therapy. Methods & clinical development 20:298-3112021Targeted epigenetic repression by CRISPR/dSaCas9 suppresses pathogenic expression in FSHD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharis L Himeda,Takako I Jones,Peter L JonesPubMed 33511244
- Biochemistry and biophysics reports 25:1008892021Pilocarpine-induced seizures associate with modifications of LSD1/CoREST/HDAC1/2 epigenetic complex and repressive chromatin in mice hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVerónica Noches,Carlos Rivera,Marcela P González,Gianluca Merello,Montserrat Olivares-Costa,María Estela AndrésPubMed 33426312
- Cell reports 34:1085742021Zinc Finger Protein SALL4 Functions through an AT-Rich Motif to Regulate Gene Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNikki R Kong,Mahmoud A Bassal,Hong Kee Tan,Jesse V Kurland,Kol Jia Yong,John J Young,Yang Yang,Fudong Li,Jonathan D Lee,Yue Liu,Chan-Shuo Wu,Alicia Stein,Hongbo R Luo,Leslie E Silberstein,Martha L Bulyk,Daniel G Tenen,Li ChaiPubMed 33406418
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CBX5 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link P45973
Consider this alternative
Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Rhesus monkey, Chinese hamster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.03-0.1 µg/mL | Notes Primary incubation 1 hour at room temperature reccomended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Rhesus monkey, Chinese hamster | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Rhesus monkey, Chinese hamster | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph) (PubMed:19783980). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR) (PubMed:19783980). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1 (PubMed:19783980, PubMed:20231385). Required for the formation of the inner centromere (PubMed:20231385).
Alternative names
HP1A, CBX5, Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha
Recommended products
Goat Polyclonal HP1 alpha antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 41 publications. Immunogen corresponding to Synthetic Peptide within Human CBX5 aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CBX5 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link P45973
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab77256 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HP1 alpha also known as HP1a or HP1 protein is a well-studied member of the heterochromatin protein 1 family. It plays a mechanical role in chromatin organization and gene regulation by binding to histone H3 when it's methylated at lysine 9 (H3K9me). This protein has a molecular mass of approximately 22-25 kDa. HP1 alpha is commonly expressed in the nuclei of eukaryotic cells where it localizes to heterochromatin regions and influences chromatin compaction.
- Biological function summary
Heterochromatin protein 1 alpha acts as a modulator of gene silencing. It is part of a larger protein complex that contains various chromatin modifiers responsible for maintaining the silent state of certain genes. By mediating these interactions HP1 alpha helps regulate transcription replication timing and DNA repair processes. This regulation is essential for the structural maintenance of heterochromatin and plays a part in chromosomal stability.
- Pathways
HP1 alpha is integral in the epigenetic regulation pathway along with other well-known proteins including SUV39H1 and SUV39H2 which are histone methyltransferases. HP1 alpha also links to the DNA damage response pathway where it recruits repair factors to maintain genomic integrity. Through these pathways HP1 alpha interacts closely with proteins like BRCA1 emphasizing its role in cellular homeostasis and response to DNA damage.
- Associated diseases and disorders
Researchers associate HP1 alpha with cancer and developmental disorders. Alterations in HP1 alpha expression or function can lead to disruptions in chromatin organization contributing to oncogenesis by affecting tumor suppressor genes. Furthermore HP1 alpha links to disorders like ICF syndrome through its relationship with the DNA methylation process where it interacts with proteins such as DNMT3B. Understanding these connections provides insights into therapeutic approaches targeting epigenetic modifications in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Flow Cytometry - Anti-HP1 alpha antibody (ab77256)
Flow cytometric analysis HeLa cells labeling HP1 alpha with ab77256 at 10 ug/mL (blue line). Cells were fixed with paraformaldehyde fixed and permeabilized with 0.5% Triton. Alexa Fluor 488 at 2ug/mL was used as the secondary antibody. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot - Anti-HP1 alpha antibody (ab77256)
All lanes: Western blot - Anti-HP1 alpha antibody (ab77256) at 0.03 µg/mL
Lane 1: A43N cell lysate at 35 µg
Lane 2: K562 cell lysate at 35 µg
Predicted band size: 22 kDa
Observed band size: 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody (ab77256)
Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling HP1 alpha with ab77256 at 10ug/ml for 1hr, followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
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