AB77256

Anti-HP1 alpha antibody

Name: Anti-HP1 alpha antibody (ab77256) | Abcam
Brand: Abcam Limited
SKU: AB77256
Price: 450 USD
Availability: InStock
Rating: 4 (12 reviews)

(12 Reviews)

(50 Publications)

Goat Polyclonal HP1 alpha antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 50 publications. Immunogen corresponding to Synthetic Peptide within Human CBX5 aa 50-150.

View Alternative Names

HP1A, CBX5, Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha

3 Images

Flow Cytometry - Anti-HP1 alpha antibody (AB77256)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-HP1 alpha antibody (AB77256)

Flow cytometric analysis HeLa cells labeling HP1 alpha with ab77256 at 10 ug/mL (blue line). Cells were fixed with paraformaldehyde fixed and permeabilized with 0.5% Triton. Alexa Fluor 488 at 2ug/mL was used as the secondary antibody. IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody (AB77256)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody (AB77256)

Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling HP1 alpha with ab77256 at 10ug/ml for 1hr, followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Supplier Data

Western blot - Anti-HP1 alpha antibody (AB77256)

All lanes:

Western blot - Anti-HP1 alpha antibody (ab77256) at 0.03 µg/mL

Lane 1:

A43N cell lysate at 35 µg

Lane 2:

K562 cell lysate at 35 µg

Predicted band size: 22 kDa

Observed band size: 25 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human CBX5 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P45973

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "Primary incubation 1 hour at room temperature reccomended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab77256 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HP1 alpha also known as HP1a or HP1 protein is a well-studied member of the heterochromatin protein 1 family. It plays a mechanical role in chromatin organization and gene regulation by binding to histone H3 when it's methylated at lysine 9 (H3K9me). This protein has a molecular mass of approximately 22-25 kDa. HP1 alpha is commonly expressed in the nuclei of eukaryotic cells where it localizes to heterochromatin regions and influences chromatin compaction.

Biological function summary

Heterochromatin protein 1 alpha acts as a modulator of gene silencing. It is part of a larger protein complex that contains various chromatin modifiers responsible for maintaining the silent state of certain genes. By mediating these interactions HP1 alpha helps regulate transcription replication timing and DNA repair processes. This regulation is essential for the structural maintenance of heterochromatin and plays a part in chromosomal stability.

Pathways

HP1 alpha is integral in the epigenetic regulation pathway along with other well-known proteins including SUV39H1 and SUV39H2 which are histone methyltransferases. HP1 alpha also links to the DNA damage response pathway where it recruits repair factors to maintain genomic integrity. Through these pathways HP1 alpha interacts closely with proteins like BRCA1 emphasizing its role in cellular homeostasis and response to DNA damage.

Researchers associate HP1 alpha with cancer and developmental disorders. Alterations in HP1 alpha expression or function can lead to disruptions in chromatin organization contributing to oncogenesis by affecting tumor suppressor genes. Furthermore HP1 alpha links to disorders like ICF syndrome through its relationship with the DNA methylation process where it interacts with proteins such as DNMT3B. Understanding these connections provides insights into therapeutic approaches targeting epigenetic modifications in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression (PubMed : 40440427). In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph) (PubMed : 19783980). Also recognizes and binds histone H1.4 methylated at 'Lys-26' (H1.4K26me) (PubMed : 16127177). Excluded from chromatin when histone H1.4 is Simultaneously methylated at Lys-26 (H1.4K26me) and phosphorylated at Ser-27 (H1.4S27Ph) (PubMed : 16127177). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR) (PubMed : 19783980). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1 (PubMed : 19783980, PubMed : 20231385). Required for the formation of the inner centromere (PubMed : 20231385).

See full target information CBX5

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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 67:101204 PubMed40609276

2025

SKI regulates rRNA transcription and pericentromeric heterochromatin to ensure centromere integrity and genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Víctor Pola-Véliz,David Carrero,Eduardo A Sagredo,Víctor Inostroza,Claudio Cappelli,Solange Rivas,Mirit Bitrán,Evelyn Zambrano,Evelin Gonzalez,Fernanda Morales,Marcia Manterola,Martín Montecino,Ricardo Armisén,Katherine Marcelain

Nature communications 16:5066 PubMed40450002

2025

The HP1 box of KAP1 organizes HP1α for silencing of endogenous retroviral elements in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nitika Gaurav,Ryan O'Hara,Usman Hyder,Weihua Qin,Cheenou Her,Hector Romero,Amarjeet Kumar,Maria J Marcaida,Rohit K Singh,Ruud Hovius,Karthik Selvam,Jiuyang Liu,Sara Martire,Yuhang Yao,Ashwini Challa,Matteo Dal Peraro,Beat Fierz,Hidetoshi Kono,M Cristina Cardoso,Galia T Debelouchina,Heinrich Leonhardt,Iván D'Orso,Laura A Banaszynski,Tatiana G Kutateladze

EMBO reports 25:3486-3505 PubMed38890452

2024

PHF2-mediated H3K9me balance orchestrates heterochromatin stability and neural progenitor proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Aguirre,Stella Pappa,Núria Serna-Pujol,Natalia Padilla,Simona Iacobucci,A Silvina Nacht,Guillermo P Vicent,Albert Jordan,Xavier de la Cruz,Marian A Martínez-Balbás

iScience 27:109629 PubMed38616920

2024

Y chromosome damage underlies testicular abnormalities in ATR-X syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Nayla Y León,Thanh Nha Uyen Le,Andrew Garvie,Lee H Wong,Stefan Bagheri-Fam,Vincent R Harley

Nature communications 14:6700 PubMed37872148

2023

Phase separation of BuGZ regulates gut regeneration and aging through interaction with mA regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoqiao Zhang,Kai Deng,Mengyou Liu,Shengye Yang,Wei Xu,Tong Feng,Minwen Jie,Zhiming Liu,Xiao Sheng,Haiyang Chen,Hao Jiang

Bioscience reports 43: PubMed37782747

2023

Writers and readers of H3K9me2 form distinct protein networks during the cell cycle that include candidates for H3K9 mimicry.

Applications

Unspecified application

Species

Unspecified reactive species

Gareth Pollin,Thiago M De Assuncao,Salomao Doria Jorge,Young-In Chi,M Cristine Charlesworth,Benjamin Madden,Juan Iovanna,Michael T Zimmermann,Raul Urrutia,Gwen Lomberk

BMC cancer 22:1166 PubMed36368958

2022

The H2A.Z-KDM1A complex promotes tumorigenesis by localizing in the nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Yongqiang Qi,Fei Xiong,Da Wang,Bing Wang,Yongjun Chen

iScience 25:104978 PubMed36117991

2022

Confined migration induces heterochromatin formation and alters chromatin accessibility.

Applications

Unspecified application

Species

Unspecified reactive species

Chieh-Ren Hsia,Jawuanna McAllister,Ovais Hasan,Julius Judd,Seoyeon Lee,Richa Agrawal,Chao-Yuan Chang,Paul Soloway,Jan Lammerding

Nature communications 13:3525 PubMed35725842

2022

Satellite repeat transcripts modulate heterochromatin condensates and safeguard chromosome stability in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Lopes Novo,Emily V Wong,Colin Hockings,Chetan Poudel,Eleanor Sheekey,Meike Wiese,Hanneke Okkenhaug,Simon J Boulton,Srinjan Basu,Simon Walker,Gabriele S Kaminski Schierle,Geeta J Narlikar,Peter J Rugg-Gunn

Cells 11: PubMed35563684

2022

Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Koei Okazaki,Megumi Nakano,Jun-Ichirou Ohzeki,Koichiro Otake,Kazuto Kugou,Vladimir Larionov,William C Earnshaw,Hiroshi Masumoto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HP1 alpha antibody

Flow Cytometry - Anti-HP1 alpha antibody (AB77256)

Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody (AB77256)

Western blot - Anti-HP1 alpha antibody (AB77256)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

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Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (50)

Product promise