Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (AB248560)

This data was developed using ab133577, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometrric analysis of HPA1 alpha in permeabilized SH SY5Y cells, using ab133577 at a 1/10 dilution (red) or a control rabbit IgG antibody (green).

• WB

Unknown

Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (AB248560)

This data was developed using ab133577, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HP1 alpha antibody [EPR5776] (<a href='/en-us/products/primary-antibodies/hp1-alpha-antibody-epr5776-ab133577'>ab133577</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SH SY5Y cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Lane 8:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 22 kDa

false

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (AB248560)

This data was developed using the same antibody clone in a different buffer formulation (ab133577).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ K-562 (human chronic myelogenous leukemia lymphoblast) cells and 5 μg of ab ab133577[EPR5776]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (AB248560)

This data was developed using the same antibody clone in a different buffer formulation (ab133577).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ K-562 (human chronic myelogenous leukemia lymphoblast) cells and 5 μg of ab ab133577[EPR5776]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (AB248560)

This data was developed using the same antibody clone in a different buffer formulation (ab133577).

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10⁵ K-562 (human chronic myelogenous leukemia lymphoblast) cells and 5 μg of ab ab133577[EPR5776]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.