Anti-HP1 alpha antibody [RAB-C133]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-HP1 alpha antibody [RAB-C133] (AB317725)

Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of ab317725 (blue line) at a dilution of 1 : 100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1 : 1000.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [RAB-C133] (AB317725)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of ab317725 (1 : 100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom primary antibody/secondary, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

• WB

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Western blot - Anti-HP1 alpha antibody [RAB-C133] (AB317725)

Chimeric rabbit version of ab317725 was used for this WB. A primary incubation of 1h was used and protein was detected by chemiluminescence.

All lanes:

Western blot - Anti-HP1 alpha antibody [RAB-C133] (ab317725) at 0.003 µg/mL

Lane 1:

Nuclear lysates of HeLa (35ug protein in RIPA buffer)

Lane 2:

Nuclear lysates of HEK293 (35ug protein in RIPA buffer)

Lane 3:

Nuclear lysates of A431 (35ug protein in RIPA buffer)

Lane 4:

Nuclear lysates of MCF7 (35ug protein in RIPA buffer)

Lane 5:

Nuclear lysates of Jurkat (35ug protein in RIPA buffer)

Lane 6:

Nuclear lysates of K562 (35ug protein in RIPA buffer)

Secondary

All lanes:

Anti-rabbit secondary antibody

Predicted band size: 22 kDa

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