Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Rabbit Recombinant Monoclonal HP1 gamma/CBX3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (AB154871), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB
Human	Tested	Tested
Mouse	Expected	Tested
Rat	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/4000	Notes For unpurified use at 1/50 - 1/100. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Associated Products

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Target data

See full target information CBX3

Function

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).

Alternative names

Chromobox protein homolog 3, HECH, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, HP1 gamma, CBX3

Recommended products

Rabbit Recombinant Monoclonal HP1 gamma/CBX3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR10465(B)
Purification technique: Affinity purification Protein A
Specificity: The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The HP1 gamma protein also known as CBX3 is an essential component of the chromatin organization machinery. It has a molecular weight of approximately 22-25 kDa. HP1 gamma is expressed in a variety of tissues including brain liver and muscle indicating its broad functional involvement across different biological systems. It exhibits a high affinity for binding to histone H3 when it is methylated facilitating the formation of heterochromatin which is key to regulating gene expression and maintaining genome stability.

Biological function summary

HP1 gamma plays a significant role in gene silencing and chromatin structure maintenance. It forms part of a protein complex that includes chromatin remodeling enzymes and transcriptional repressors which help regulate access to genomic DNA. HP1 gamma's association with other chromatin-associated proteins helps modulate the transcriptional response to cellular stimuli ensuring appropriate gene expression patterns needed for normal cell function and development.

Pathways

HP1 gamma is involved in heterochromatin formation and maintenance pathways working alongside other proteins like SUV39H1 and HP1 alpha. Through these pathways HP1 gamma ensures the compact organization of repetitive DNA sequences and suppresses unnecessary transcription. It is also involved in the DNA damage response pathway playing a role in preserving genomic integrity by interacting with proteins such as BRCA1 and 53BP1 involved in DNA repair.

Associated diseases and disorders

HP1 gamma's dysregulation has been associated with cancer and neurological disorders. Overexpression or mutations in this protein can lead to aberrant gene silencing contributing to oncogenesis where cancer-related genes become improperly regulated. Additionally alterations in HP1 gamma function are linked to neurodegenerative diseases potentially through its interaction with proteins like MECP2 whereby changes in the chromatin environment influence neuronal gene expression and function. Therefore understanding HP1 gamma's role provides insights into potential therapeutic targets for these conditions.

Product promise

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10 product images

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 μg)
Lane 3: NIH3T3 cell lysate (20 μg)
Lane 4: HeLa cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154871 observed at 24 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

ab154871was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab154871 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted to 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Predicted band size: 20 kDa
Observed band size: 24 kDa
Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
All lanes: Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871) at 1/5000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: Mouse brain lysates at 15 µg
Lane 3: Rat brain lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling HP1 gamma/CBX3 with purified ab154871 at 1:4000 dilution (0.29 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
All lanes: Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871) at 1/1000 dilution
Lane 1: NIH/3T3 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: Molt-4 cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HP1 gamma/CBX3 with ab154871 at 1/50 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling HP1 gamma/CBX3 with ab154871 at 1/50 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154871 showing +ve staining.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.