AB249170

Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal HP1 gamma/CBX3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Chromobox protein homolog 3, HECH, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, HP1 gamma, CBX3

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling HP1 gamma/CBX3 with purified ab154871 at 1 : 4000 dilution (0.29 μg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HP1 gamma/CBX3 with ab154871 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling HP1 gamma/CBX3 with ab154871 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

All lanes:

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (ab249170) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Mouse brain lysates at 15 µg

Lane 3:

Rat brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 20 kDa

Observed band size: 21 kDa

false

Supplier Data

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : HP1 gamma/CBX3 knockout HAP1 cell lysate (20 μg)
Lane 3 : NIH3T3 cell lysate (20 μg)
Lane 4 : HeLa cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab154871 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab154871was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab154871 and ab8245 (loading control to GAPDH) were both diluted to 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (ab249170)

Predicted band size: 20 kDa

Observed band size: 24 kDa

false

Supplier Data

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154871).

All lanes:

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (ab249170) at 1/1000 dilution

Lane 1:

NIH/3T3 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Molt-4 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa

false

Unconjugated

Anti-HP1 gamma/CBX3 antibody [EPR10465(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10465(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249170 is the carrier-free version of ab154871.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HP1 gamma protein also known as CBX3 is an essential component of the chromatin organization machinery. It has a molecular weight of approximately 22-25 kDa. HP1 gamma is expressed in a variety of tissues including brain liver and muscle indicating its broad functional involvement across different biological systems. It exhibits a high affinity for binding to histone H3 when it is methylated facilitating the formation of heterochromatin which is key to regulating gene expression and maintaining genome stability.

Biological function summary

HP1 gamma plays a significant role in gene silencing and chromatin structure maintenance. It forms part of a protein complex that includes chromatin remodeling enzymes and transcriptional repressors which help regulate access to genomic DNA. HP1 gamma's association with other chromatin-associated proteins helps modulate the transcriptional response to cellular stimuli ensuring appropriate gene expression patterns needed for normal cell function and development.

Pathways

HP1 gamma is involved in heterochromatin formation and maintenance pathways working alongside other proteins like SUV39H1 and HP1 alpha. Through these pathways HP1 gamma ensures the compact organization of repetitive DNA sequences and suppresses unnecessary transcription. It is also involved in the DNA damage response pathway playing a role in preserving genomic integrity by interacting with proteins such as BRCA1 and 53BP1 involved in DNA repair.

HP1 gamma's dysregulation has been associated with cancer and neurological disorders. Overexpression or mutations in this protein can lead to aberrant gene silencing contributing to oncogenesis where cancer-related genes become improperly regulated. Additionally alterations in HP1 gamma function are linked to neurodegenerative diseases potentially through its interaction with proteins like MECP2 whereby changes in the chromatin environment influence neuronal gene expression and function. Therefore understanding HP1 gamma's role provides insights into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed : 28167679).

See full target information CBX3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] - BSA and Azide free (AB249170)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise