Rabbit Polyclonal HP1 gamma/CBX3 phospho S93 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 8 publications.
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Publications
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- Journal of cellular biochemistry 124:1603-16142023Inhibition of annexin A7 suppresses senescence-associated heterochromatin foci formation and senescence through the AMPK/mTOR pathway in human dermal fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Li,Xiaomeng Yan,Xiaoling Cui,Congyao Zhao,Zhaomin Lin,Junying MiaoPubMed 37682859
- Life science alliance 6:2023PRMT5 triggers glucocorticoid-induced cell migration in triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLara Malik Noureddine,Julien Ablain,Ausra Surmieliova-Garnès,Julien Jacquemetton,Thuy Ha Pham,Elisabetta Marangoni,Anne Schnitzler,Ivan Bieche,Bassam Badran,Olivier Trédan,Nader Hussein,Muriel Le Romancer,Coralie PoulardPubMed 37536978
- Nature communications 11:13352020Cancer immune control needs senescence induction by interferon-dependent cell cycle regulator pathways in tumours.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEllen Brenner et. al.PubMed 32165639
- EMBO reports 18:1442-14592017A post-translational modification switch controls coactivator function of histone methyltransferases G9a and GLP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCoralie Poulard et. al.PubMed 28615290
- Cancer research 76:227-382015Control of PD-L1 Expression by Oncogenic Activation of the AKT-mTOR Pathway in Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristin J Lastwika,Willie Wilson,Qing Kay Li,Jeffrey Norris,Haiying Xu,Sharon R Ghazarian,Hiroshi Kitagawa,Shigeru Kawabata,Janis M Taube,Sheng Yao,Linda N Liu,Joell J Gills,Phillip A DennisPubMed 26637667
- The EMBO journal 33:2606-222014Shigella flexneri targets the HP1γ subcode through the phosphothreonine lyase OspF.
- The Journal of clinical investigation 124:2125-352014Senescence-associated SIN3B promotes inflammation and pancreatic cancer progression.
- Nucleic acids research 40:7676-892012Heterochromatin protein 1 gamma and IκB kinase alpha interdependence during tumour necrosis factor gene transcription elongation in activated macrophages.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Rhesus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Rhesus monkey, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Rhesus monkey, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Rhesus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
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8 products for Alternative Product
Target data
Function
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).
Alternative names
Chromobox protein homolog 3, HECH, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, HP1 gamma, CBX3
Recommended products
Rabbit Polyclonal HP1 gamma/CBX3 phospho S93 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 8 publications.
Alternative names
Chromobox protein homolog 3, HECH, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, HP1 gamma, CBX3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HP1 gamma protein also known as CBX3 is an essential component of the chromatin organization machinery. It has a molecular weight of approximately 22-25 kDa. HP1 gamma is expressed in a variety of tissues including brain liver and muscle indicating its broad functional involvement across different biological systems. It exhibits a high affinity for binding to histone H3 when it is methylated facilitating the formation of heterochromatin which is key to regulating gene expression and maintaining genome stability.
- Biological function summary
HP1 gamma plays a significant role in gene silencing and chromatin structure maintenance. It forms part of a protein complex that includes chromatin remodeling enzymes and transcriptional repressors which help regulate access to genomic DNA. HP1 gamma's association with other chromatin-associated proteins helps modulate the transcriptional response to cellular stimuli ensuring appropriate gene expression patterns needed for normal cell function and development.
- Pathways
HP1 gamma is involved in heterochromatin formation and maintenance pathways working alongside other proteins like SUV39H1 and HP1 alpha. Through these pathways HP1 gamma ensures the compact organization of repetitive DNA sequences and suppresses unnecessary transcription. It is also involved in the DNA damage response pathway playing a role in preserving genomic integrity by interacting with proteins such as BRCA1 and 53BP1 involved in DNA repair.
- Associated diseases and disorders
HP1 gamma's dysregulation has been associated with cancer and neurological disorders. Overexpression or mutations in this protein can lead to aberrant gene silencing contributing to oncogenesis where cancer-related genes become improperly regulated. Additionally alterations in HP1 gamma function are linked to neurodegenerative diseases potentially through its interaction with proteins like MECP2 whereby changes in the chromatin environment influence neuronal gene expression and function. Therefore understanding HP1 gamma's role provides insights into potential therapeutic targets for these conditions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-HP1 gamma/CBX3 (phospho S93) antibody (ab45270)
All lanes: Western blot - Anti-HP1 gamma/CBX3 (phospho S93) antibody (ab45270) at 1 µg/mL
Lanes 1, 15 and 8: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 16, 2 and 9: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lanes 10, 17 and 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 11, 18 and 4: HEK293 Human embryonic kidney cell line Whole Cell Lysate at 10 µg
Lanes 12, 19 and 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 20 and 6: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lanes 21 and 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Lane 13: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate (ab28419) at 10 µg
Lane 14: SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968) at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 24 kDa, 40 kDa
Immunoprecipitation - Anti-HP1 gamma/CBX3 (phospho S93) antibody (ab45270)
HP1 gamma/CBX was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5μg of Rabbit polyclonal to HP1 gamma/CBX and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab45270.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 22kDa; HP1 gamma/CBXAll lanes: Immunoprecipitation - Anti-HP1 gamma/CBX3 (phospho S93) antibody (ab45270)
Predicted band size: 20 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HP1 gamma/CBX3 (phospho S93) antibody (ab45270)
ICC/IF image of ab45270 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45270, 5μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 (phospho S93) antibody (ab45270)
IHC image of HP1 gamma/CBX (phospho S83) staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45270, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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