Rabbit Recombinant Monoclonal HPD antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-HPD antibody [EPR5297] (AB133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--western-blot-img8131.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HPD antibody [EPR5297] (AB133515)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (AB133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130603.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (AB133515)")
![Western blot - Anti-HPD antibody [EPR5297] (AB133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--western-blot-img86585.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HPD antibody [EPR5297] (AB133515)")
![Western blot - Anti-HPD antibody [EPR5297] (AB133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--western-blot-img106579.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HPD antibody [EPR5297] (AB133515)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (AB133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17647.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (AB133515)")
Publications
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- Med (New York, N.Y.) 2:395-4102021Isotope tracing reveals glycolysis and oxidative metabolism in childhood tumors of multiple histologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKendra Johnston,Panayotis Pachnis,Alpaslan Tasdogan,Brandon Faubert,Lauren G Zacharias,Hieu Sy Vu,Laurie Rodgers-Augustyniak,Allison Johnson,Fang Huang,Sean Ricciardo,Zhiyu Zhao,Thomas P Mathews,Tanya Watt,Patrick Leavey,Ralph J DeBerardinisPubMed 33860280
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Expected | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes For unpurified use at 1/250-1/50 The rat recommendation is based on the WB results. This antibody may not be suitable for IHC with rat samples. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, one of the steps in tyrosine catabolism.
Alternative names
PPD, HPD, 4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPPDase
Recommended products
Rabbit Recombinant Monoclonal HPD antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5297
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.57 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-HPD antibody [EPR5297] (ab133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--western-blot-img8131.jpg "Western blot - Anti-HPD antibody [EPR5297] (ab133515)")
Western blot - Anti-HPD antibody [EPR5297] (ab133515)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution
All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130603.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HPD with purified ab133515 at 1:2000 dilution (0.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
![Western blot - Anti-HPD antibody [EPR5297] (ab133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--western-blot-img86585.jpg "Western blot - Anti-HPD antibody [EPR5297] (ab133515)")
Western blot - Anti-HPD antibody [EPR5297] (ab133515)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-HPD antibody [EPR5297] (ab133515) at 1/5000 dilution
All lanes: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 22 kDa, 25 kDa, 45 kDa
Observed band size: 35 kDa
![Western blot - Anti-HPD antibody [EPR5297] (ab133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--western-blot-img106579.jpg "Western blot - Anti-HPD antibody [EPR5297] (ab133515)")
Western blot - Anti-HPD antibody [EPR5297] (ab133515)
All lanes: Western blot - Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: Fetal liver tissue lysate at 10 µg
Lane 3: Human kidney lysate at 10 µg
SecondaryAll lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17647.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HPD with unpurified ab133515 at 1/250 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-HPD antibody [EPR5297] (ab133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--oi-rd-scanning-img97634.jpg "OI-RD Scanning - Anti-HPD antibody [EPR5297] (ab133515)")
OI-RD Scanning - Anti-HPD antibody [EPR5297] (ab133515)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
![OI-RD Scanning - Anti-HPD antibody [EPR5297] (ab133515), expandable thumbnail](https://content.abcam.com/products/images/hpd-antibody-epr5297-ab133515--oi-rd-scanning-img97634.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-HPD antibody [EPR5297] (ab133515)")
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