Anti-HPD antibody [EPR5297]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (AB133515)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HPD with unpurified ab133515 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (AB133515)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HPD with purified ab133515 at 1 : 2000 dilution (0.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-HPD antibody [EPR5297] (AB133515)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution

All lanes:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

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• WB

Lab

Western blot - Anti-HPD antibody [EPR5297] (AB133515)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-HPD antibody [EPR5297] (ab133515) at 1/5000 dilution

All lanes:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa,25 kDa,45 kDa

Observed band size: 35 kDa

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• WB

Unknown

Western blot - Anti-HPD antibody [EPR5297] (AB133515)

All lanes:

Western blot - Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Fetal liver tissue lysate at 10 µg

Lane 3:

Human kidney lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 45 kDa

true

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HPD antibody [EPR5297] (AB133515)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.