Anti-HPDL antibody [EPR11691] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-HPDL antibody [EPR11691] - BSA and Azide free (AB249829)

This data was developed using ab174841, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, labeling HPDL, immunoprecipitated using ab174841 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-HPDL antibody [EPR11691] - C-terminal (<a href='/en-us/products/primary-antibodies/hpdl-antibody-epr11691-c-terminal-ab174841'>ab174841</a>)

Predicted band size: 39 kDa

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• WB

Supplier Data

Western blot - Anti-HPDL antibody [EPR11691] - BSA and Azide free (AB249829)

This data was developed using ab174841, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (<a href='/en-us/products/primary-antibodies/hpdl-antibody-epr11691-c-terminal-ab174841'>ab174841</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Predicted band size: 39 kDa

false

• WB

Lab

Western blot - Anti-HPDL antibody [EPR11691] - BSA and Azide free (AB249829)

This data was developed using the same antibody clone in a different buffer formulation (ab174841).

Lanes 1-3 : Merged signal (red and green). Green - ab174841 observed at 39 kDa. Red - loading control ab7291 observed at 50 kDa.

ab174841 Anti-HPDL antibody [EPR11691] - C-terminal was shown to specifically react with HPDL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265868 (knockout cell lysate ab257995) was used. Wild-type and HPDL knockout samples were subjected to SDS-PAGE. ab174841 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (<a href='/en-us/products/primary-antibodies/hpdl-antibody-epr11691-c-terminal-ab174841'>ab174841</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

HPDL knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human HPDL knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-hpdl-knockout-hela-cell-line-ab265868'>ab265868</a>)

Lane 3:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

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