Anti-HPRT antibody (ab10479) is a rabbit polyclonal antibody that is used to detect HPRT in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with HPRT knockout cell line validation
Images
Publications
Filter by
- Life science alliance 5:2021Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Riera-Tur,Tillman Schäfer,Daniel Hornburg,Archana Mishra,Miguel da Silva Padilha,Lorena Fernández-Mosquera,Dennis Feigenbutz,Patrick Auer,Matthias Mann,Wolfgang Baumeister,Rüdiger Klein,Felix Meissner,Nuno Raimundo,Rubén Fernández-Busnadiego,Irina DudanovaPubMed 34933920
- Environmental health perspectives 129:970062021Exposure of Mice during Spermatogenesis: A Role of Inhibitor Kinase 2 in Pro-Opiomelanocortin Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLianglin Qiu,Minjie Chen,Xiaoke Wang,Sufang Chen,Zhekang YingPubMed 34495743
- Molecular therapy oncolytics 23:107-1232021Oleanolic acid blocks the purine salvage pathway for cancer therapy by inactivating SOD1 and stimulating lysosomal proteolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Liu,Xing Jin,Guanzhen Yu,Mingsong Wang,Lei Liu,Wenjuan Zhang,Jia Wu,Fengying Wang,Jing Yang,Qin Luo,Lili Cai,Xi Yang,Xisong Ke,Yi Qu,Zhenye Xu,Lijun Jia,Wen-Lian ChenPubMed 34703880
- Scientific reports 11:108872021IMPDH2 and HPRT expression and a prognostic significance in preoperative and postoperative patients with osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParunya Chaiyawat,Areerak Phanphaisarn,Nutnicha Sirikaew,Jeerawan Klangjorhor,Viraporn Thepbundit,Pimpisa Teeyakasem,Phichayut Phinyo,Dumnoensun Pruksakorn,Jongkolnee SettakornPubMed 34035425
- Cell death & disease 12:2772021Rewiring of purine metabolism in response to acidosis stress in glioma stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Xu,Liping Wang,Qingce Zang,Shanshan Li,Limei Li,Zhixing Wang,Jiuming He,Boqin Qiang,Wei Han,Ruiping Zhang,Xiaozhong Peng,Zeper AblizPubMed 33723244
- Cancer cell international 20:3752020Evaluation of the upregulation and surface expression of hypoxanthine guanine phosphoribosyltransferase in acute lymphoblastic leukemia and Burkitt's B cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichelle H Townsend et. al.PubMed 32782434
- Aging 12:8820-88362020LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson's disease via the HPRT1-mediated Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Jiang et. al.PubMed 32434961
- Experimental cell research 388:1118402020Multigene human artificial chromosome vector delivery with herpes simplex virus 1 amplicons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Yl Chan et. al.PubMed 31930965
- Nature genetics 52:84-942020RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames D Thomas et. al.PubMed 31911676
- eLife 8:2019Impaired lysosomal acidification triggers iron deficiency and inflammation in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKing Faisal Yambire et. al.PubMed 31793879
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Chicken | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Gerbil | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Gerbil, Chinese hamster | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Gerbil, Chinese hamster | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Alternative names
HPRT, HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase
Recommended products
Anti-HPRT antibody (ab10479) is a rabbit polyclonal antibody that is used to detect HPRT in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with HPRT knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab10479 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab133242.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-HPRT antibody (ab10479) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of HPRT?
Anti-HPRT (ab10479) specifically detects a band for HPRT (UniProt: P00492) at a molecular weight of 24kDa.
Trusted by the scientific community
Anti-HPRT (ab10479) was first used in a scientific publication in 2004 and has been cited over 40 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-HPRT antibody (ab10479) has been confirmed by Western blot testing in HPRT1 Knockout HAP1 cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Western blot - Anti-HPRT antibody (ab10479)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: HPRT1 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: A431 whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10479 observed at 25 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.ab10479 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were examined. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. ab10479 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HPRT antibody (ab10479)
Predicted band size: 25 kDa
Western blot - Anti-HPRT antibody (ab10479)
All lanes: Western blot - Anti-HPRT antibody (ab10479) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
Lane 3: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa
Western blot - Anti-HPRT antibody (ab10479)
All lanes: Western blot - Anti-HPRT antibody (ab10479) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa, 28 kDa
Western blot - Anti-HPRT antibody (ab10479)
All lanes: Western blot - Anti-HPRT antibody (ab10479) at 1/500 dilution
All lanes: Western blot - Recombinant Human HPRT protein (Recombinant Human HPRT protein ab97411) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 1min
Western blot - Anti-HPRT antibody (ab10479)
All lanes: Western blot - Anti-HPRT antibody (ab10479) at 1/500 dilution
All lanes: Western blot - Recombinant Human HPRT protein (Recombinant Human HPRT protein ab97411) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 1min
Western blot - Anti-HPRT antibody (ab10479)
Lane 1: HeLa whole cell lysate (20 µg)
Lane 2: NIH/3T3 whole cell lysate (20 µg)
Lane 3: PC12 whole cell lysate (20 µg)
Lane 4: Wild-type HAP1 whole cell lysate (20 µg)
Lane 5: Empty Lane
Lane 6: HPRT1 knockout HAP1 whole cell lysate (20 µg)
Lanes 1 - 6: Merged signal (red and green). Green - ab10479 observed at 24 kDa. Red - loading control Anti-beta Catenin antibody [15B8] ab6301 observed at 95 kDa.
Gel type: MES
Blocking buffer: LiCOR blocking buffer
ab10479 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were examined. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. ab10479 and Anti-beta Catenin antibody [15B8] ab6301 (Beta catenin - Loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800RD) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680CW) preadsorbed secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-HPRT antibody (ab10479) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: NIH/3T3 whole cell lysate at 20 µg
Lane 3: PC12 whole cell lysate at 20 µg
Lane 4: Wild-type HAP1 whole cell lysate at 20 µg
Lane 6: HPRT1 knockout HAP1 whole cell lysate at 20 µg
SecondaryLanes 1 - 6: Goat anti-Mouse IgG H&L (IRDye® 680CW) preadsorbed at 1/10000 dilution
Lanes 1 - 6: Goat anti-Rabbit IgG H&L (IRDye® 800RD) preadsorbed at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 24 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HPRT antibody (ab10479)
ab10479 staining HPRT in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab10479 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com