Anti-HPRT antibody

• IP

Supplier Data

Immunoprecipitation - Anti-HPRT antibody (AB226412)

HPRT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226412 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226412 at 1 μg/ml.

Lane 1 : ab226412 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-HPRT antibody (ab226412)

Predicted band size: 25 kDa

false

• WB

Supplier Data

Western blot - Anti-HPRT antibody (AB226412)

Cell lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-HPRT antibody (ab226412) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 25 kDa

true

Exposure time: 10s