AB133242

Anti-HPRT antibody [EPR5298]

Name: Anti-HPRT antibody [EPR5298] (ab133242) | Abcam
Brand: Abcam Limited
SKU: AB133242
Price: 355 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(2 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal HPRT antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

HPRT, HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase

2 Images

Lab

Western blot - Anti-HPRT antibody [EPR5298] (AB133242)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : HPRT1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : A431 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab133242 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133242 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were used. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. ab133242 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 and 1/10,000 respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HPRT antibody [EPR5298] (ab133242)

Predicted band size: 25 kDa,42 kDa,44 kDa

false

Unknown

Western blot - Anti-HPRT antibody [EPR5298] (AB133242)

All lanes:

Western blot - Anti-HPRT antibody [EPR5298] (ab133242) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

Mouse brain lysate

Lane 3:

Rat brain lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5298

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.

See full target information HPRT1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:795 PubMed39500875

2024

Metformin improves HPRT1-targeted purine metabolism and repairs NR4A1-mediated autophagic flux by modulating FoxO1 nucleocytoplasmic shuttling to treat postmenopausal osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keda Yang,Xiaochuan Wang,Chi Zhang,Dian Liu,Lin Tao

Journal of atherosclerosis and thrombosis 30:74-86 PubMed35314564

2022

Acute Cholesterol-Lowering Effect of Exendin-4 in Ldlr and C57BL/6J Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mika Hori,Yukiko Hasegawa,Yoshitaka Hayashi,Tomoko Nakagami,Mariko Harada-Shiba

Frontiers in medicine 9:820817 PubMed35308522

2022

Dramatic Effect of Botulinum Toxin Type A on Hypertrophic Scar: A Promising Therapeutic Drug and Its Mechanism Through the SP-NK1R Pathway in Cutaneous Neurogenic Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Shunuo Zhang,Ke Li,Zhixi Yu,Jun Chai,Zheng Zhang,Yixin Zhang,Peiru Min

Nature communications 12:6176 PubMed34702840

2021

The impact of physiological metabolite levels on serine uptake, synthesis and utilization in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Hennequart,Christiaan F Labuschagne,Mylène Tajan,Steven E Pilley,Eric C Cheung,Nathalie M Legrave,Paul C Driscoll,Karen H Vousden

Molecular oncology 15:1921-1941 PubMed33793068

2021

The evolutionarily conserved long non-coding RNA LINC00261 drives neuroendocrine prostate cancer proliferation and metastasis via distinct nuclear and cytoplasmic mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca L Mather,Abhijit Parolia,Sandra E Carson,Erik Venalainen,David Roig-Carles,Mustapha Jaber,Shih-Chun Chu,Ilaria Alborelli,Rebecca Wu,Dong Lin,Noushin Nabavi,Elena Jachetti,Mario P Colombo,Hui Xue,Perla Pucci,Xinpei Ci,Cheryl Hawkes,Yinglei Li,Hardev Pandha,Igor Ulitsky,Crystal Marconett,Luca Quagliata,Wei Jiang,Ignacio Romero,Yuzhuo Wang,Francesco Crea

View all publications

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