Rabbit Recombinant Monoclonal HPRT antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (AB240051), expandable thumbnail](https://content.abcam.com/products/images/hprt-antibody-epr5298-bsa-and-azide-free-ab240051--western-blot-img174223.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (AB240051)")
![Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (AB240051), expandable thumbnail](https://content.abcam.com/products/images/hprt-antibody-epr5298-bsa-and-azide-free-ab240051--western-blot-img136545.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (AB240051)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Alternative names
HPRT, HPRT1, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase
Recommended products
Rabbit Recombinant Monoclonal HPRT antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5298
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240051 is the carrier-free version of Anti-HPRT antibody [EPR5298] ab133242.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051), expandable thumbnail](https://content.abcam.com/products/images/hprt-antibody-epr5298-bsa-and-azide-free-ab240051--western-blot-img174223.jpg "Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)")
Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
All lanes: Western blot - Anti-HPRT antibody [EPR5298] (Anti-HPRT antibody [EPR5298] ab133242) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Mouse brain lysate
Lane 3: Rat brain lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 25 kDa
![Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051), expandable thumbnail](https://content.abcam.com/products/images/hprt-antibody-epr5298-bsa-and-azide-free-ab240051--western-blot-img136545.jpg "Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)")
Western blot - Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)
This data was developed using Anti-HPRT antibody [EPR5298] ab133242, the same antibody clone in a different buffer formulation.
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: HPRT1 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: A431 whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-HPRT antibody [EPR5298] ab133242 observed at 25 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-HPRT antibody [EPR5298] ab133242 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were used. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. Anti-HPRT antibody [EPR5298] ab133242 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 and 1/10,000 respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HPRT antibody [EPR5298] (Anti-HPRT antibody [EPR5298] ab133242)
Predicted band size: 25 kDa, 42 kDa, 44 kDa
Downloads
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