Anti-HPRT antibody [EPR5299] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPRT antibody [EPR5299] - BSA and Azide free (AB236055)

Unpurified ab109021 at 1/50 dilution staining HPRT in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109021).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPRT antibody [EPR5299] - BSA and Azide free (AB236055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling HPRT with Purified ab109021 at 1 : 150 dilution (1.19 �g/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylinwas used as a counterstainThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109021).

• IP

Unknown

Immunoprecipitation - Anti-HPRT antibody [EPR5299] - BSA and Azide free (AB236055)

ab109021 (purified) at 1 : 30 dilution (2�g) immunoprecipitating HPRT in Rat brain lysate.
Lane 1 (input) : Rat brain lysate 10�g
Lane 2 (+) : ab109021 & Rat brain lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109021 in Rat brain lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109021).

All lanes:

Immunoprecipitation - Anti-HPRT antibody [EPR5299] (<a href='/en-us/products/primary-antibodies/hprt-antibody-epr5299-ab109021'>ab109021</a>)

Predicted band size: 25 kDa

false

• WB

Supplier Data

Western blot - Anti-HPRT antibody [EPR5299] - BSA and Azide free (AB236055)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : HPRT1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : A431 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109021 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109021 was shown to specifically react with HPRT1 in wild-type HAP1 cells. No band was observed when HPRT1 knockout samples were examined. Wild-type and HPRT1 knockout samples were subjected to SDS-PAGE. ab109021 and ab8245 (Mouse anti GAPDH loading control) were both diluted at 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109021).

All lanes:

Western blot - Anti-HPRT antibody [EPR5299] (<a href='/en-us/products/primary-antibodies/hprt-antibody-epr5299-ab109021'>ab109021</a>)

Predicted band size: 25 kDa

false