Anti-HPS1 antibody [EP8429(2)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] - BSA and Azide free (AB284824)

This data was developed using ab174301, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling HPS1 with ab174301 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] - BSA and Azide free (AB284824)

This data was developed using ab174301, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular cancer labeling HPS1 with ab174301 at 1/500 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HPS1 antibody [EP8429(2)] - BSA and Azide free (AB284824)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 cells labelling HPS1 with ab174301 at 1/30 dilution (10 µg/mL) (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HPS1 antibody [EP8429(2)] - BSA and Azide free (AB284824)

This data was developed using ab174301, the same antibody clone in a different buffer.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 cells labelling HPS1 with ab174301 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] - BSA and Azide free (AB284824)

This data was developed using ab174301, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Mouse kidney labeling HPS1 with ab174301 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] - BSA and Azide free (AB284824)

This data was developed using ab174301, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded Rat kidney labeling HPS1 with ab174301 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-HPS1 antibody [EP8429(2)] - BSA and Azide free (AB284824)

This data was developed using ab174301, the same antibody clone in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HPS1 antibody [EP8429(2)] (<a href='/en-us/products/primary-antibodies/hps1-antibody-ep84292-ab174301'>ab174301</a>) at 1/1000 dilution

Lane 1:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A375 (Human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 79 kDa

false

Exposure time: 60s