Mouse Recombinant Monoclonal Human papillomavirus type 59 L1 protein antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - human papillomavirus 59, Recombinant fragment - human papillomavirus 59 samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (AB322271), expandable thumbnail](https://content.abcam.com/products/images/hpv-59-l1-antibody-frhpv59l16e12-ab322271--immunocytochemistry-immunofluorescence-img438922.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (AB322271)")
![Indirect ELISA - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (AB322271), expandable thumbnail](https://content.abcam.com/products/images/hpv-59-l1-antibody-frhpv59l16e12-ab322271--indirect-elisa-img438923.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (AB322271)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | I-ELISA | |
|---|---|---|
| human papillomavirus 59 | Predicted | Predicted |
| Recombinant fragment - human papillomavirus 59 | Not recommended | Tested |
| Transfected cell line - human papillomavirus 59 | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - human papillomavirus 59 | Dilution info 1/1994 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species human papillomavirus 59 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - human papillomavirus 59 | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - human papillomavirus 59 | Dilution info 1000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species human papillomavirus 59 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - human papillomavirus 59 | Dilution info - | Notes - |
Mouse Recombinant Monoclonal Human papillomavirus type 59 L1 protein antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - human papillomavirus 59, Recombinant fragment - human papillomavirus 59 samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- FR_αHPV59L1_6E12
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (ab322271), expandable thumbnail](https://content.abcam.com/products/images/hpv-59-l1-antibody-frhpv59l16e12-ab322271--immunocytochemistry-immunofluorescence-img438922.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (ab322271)")
Immunocytochemistry/ Immunofluorescence - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (ab322271)
HPV 59 L1 Immunocytochemistry/ Immunofluorescence staining using mouse Anti-HPV 59 L1 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 59 L1 protein with ab322271 at 1/2000 (0.49ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 59 (HPV59) L1 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: ab322271 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.
-ve control 2: Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.![Indirect ELISA - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (ab322271), expandable thumbnail](https://content.abcam.com/products/images/hpv-59-l1-antibody-frhpv59l16e12-ab322271--indirect-elisa-img438923.jpg "Indirect ELISA - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (ab322271)")
Indirect ELISA - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] (ab322271)
Indirect ELISA analysis of ab322271 at 2 ug/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1:1000 dilution.
Antigen: HPV 59 L1 protein,HPV 18 L1 protein,HPV 16 L1 protein,HPV 6B L1 protein,HPV 11 L1 protein.
Antigen concentration: 1000-0 ug/ml
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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