Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] - BSA and Azide free
- Recombinant
- What is this?
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![Immunocytochemistry/ Immunofluorescence - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] - BSA and Azide free (AB324798)](https://content.abcam.com/products/images/hpv-59-l1-antibody-frhpv59l16e12-bsa-and-azide-free-ab324798--immunocytochemistry-immunofluorescence-img438922.jpg/_jcr_content/renditions/webp-768.webp)
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] - BSA and Azide free (AB324798)
This data was developed using ab322271, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 59 L1 protein with ab322271 at 1/2000 (0.49ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 59 (HPV59) L1 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1 : ab322271 at 1/50 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution. -ve control 2 : ab9106 Anti-Myc Rabbit polyclonal antibody at 1/200 dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
![Indirect ELISA - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] - BSA and Azide free (AB324798)](https://content.abcam.com/products/images/hpv-59-l1-antibody-frhpv59l16e12-bsa-and-azide-free-ab324798--indirect-elisa-img438923.jpg/_jcr_content/renditions/webp-768.webp)
- I-ELISA
Supplier Data
Indirect ELISA - Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] - BSA and Azide free (AB324798)
This data was developed using ab322271, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of ab322271 at 2 ug/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.
Antigen : HPV 59 L1 protein, HPV 18 L1 protein, HPV 16 L1 protein, HPV 6B L1 protein, HPV 11 L1 protein.
Antigen concentration : 1000-0 ug/ml
Reactivity data
Product details
ab324798 is the carrier-free version of ab322271.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Product protocols
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com