AB100968

Anti-HPV11 E2 antibody [10E12]

Name: Anti-HPV11 E2 antibody [10E12] (ab100968) | Abcam
Brand: Abcam Limited
SKU: AB100968

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(3 Publications)

Mouse Monoclonal VE2 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human papillomavirus samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to human papillomavirus 11 E2.

View Alternative Names

Regulatory protein E2, E2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (AB100968)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (AB100968)

Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells.
IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.

Unknown

Western blot - Anti-HPV11 E2 antibody [10E12] (AB100968)

All lanes:

Western blot - Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution

Lanes 1 and 4:

lysate of U20S cells transfected with plasmid expressing HPV11 E2 protein

Lane 2:

lysate of plain U20S cells

Lane 3:

lysate of plain HeLa cells

Lane 5:

marker

Predicted band size: 42 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10E12

Isotype

IgG1

Carrier free

Reacts with

Human papillomavirus

Applications

ELISA, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to human papillomavirus 11 E2.

P04015

Epitope

Mapped to amino acids 202-284

Specificity

Reacts in WB, and ELISA with HPV11 native E2 protein as well as recombinant E2 protein expressed in bacteria and Cos 7 cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/32000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from bacteria by affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.

See full target information E2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Transgenic research 32:67-76 PubMed36826606

2023

Transgenic HPV11-E2 protein modulates URR activity in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Shubei Wang,Vera Gramm,Elke Laport,Tim Holland-Letz,Angel Alonso,Johannes Schenkel

The Journal of general virology 97:2989-3006 PubMed27655016

2016

Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission rather than a virological synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Asuka Nanbo,Kunihiro Kachi,Hironori Yoshiyama,Yusuke Ohba

Virology journal 12:104 PubMed26148509

2015

DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells.

Applications

Species

Unspecified reactive species

Piia Kivipõld,Liisi Võsa,Mart Ustav,Reet Kurg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HPV11 E2 antibody [10E12]

Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (AB100968)

Western blot - Anti-HPV11 E2 antibody [10E12] (AB100968)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise