Mouse Monoclonal VE2 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human papillomavirus samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to human papillomavirus 11 E2.
Images
![Western blot - Anti-HPV11 E2 antibody [10E12] (AB100968), expandable thumbnail](https://content.abcam.com/products/images/hpv11-e2-antibody-10e12-ab100968--western-blot-img15656.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HPV11 E2 antibody [10E12] (AB100968)")
![Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (AB100968), expandable thumbnail](https://content.abcam.com/products/images/hpv11-e2-antibody-10e12-ab100968--immunocytochemistry-immunofluorescence-img125062.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (AB100968)")
Publications
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- Transgenic research 32:67-762023Transgenic HPV11-E2 protein modulates URR activity in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShubei Wang,Vera Gramm,Elke Laport,Tim Holland-Letz,Angel Alonso,Johannes SchenkelPubMed 36826606
- The Journal of general virology 97:2989-30062016Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission rather than a virological synapse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsuka Nanbo et. al.PubMed 27655016
- Virology journal 12:1042015DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells.Applications:IFReactive species:Unspecified reactive speciesPiia Kivipõld,Liisi Võsa,Mart Ustav,Reet KurgPubMed 26148509
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to human papillomavirus 11 E2. Database link P04015
Reactivity data
Select an application| ELISA | WB | ICC/IF | IP | |
|---|---|---|---|---|
| Human papillomavirus | Expected | Tested | Tested | Not recommended |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus | Dilution info 1/32000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus | Dilution info - | Notes - |
Target data
Function
Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.
Additional Targets
HPV11 E2
Alternative names
Regulatory protein E2, E2
Recommended products
Mouse Monoclonal VE2 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human papillomavirus samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to human papillomavirus 11 E2.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to human papillomavirus 11 E2. Database link P04015
- Clone number
- 10E12
- Purification technique
- Affinity purification Protein G
- Specificity
Reacts in WB, and ELISA with HPV11 native E2 protein as well as recombinant E2 protein expressed in bacteria and Cos 7 cells.
- Epitope
- Mapped to amino acids 202-284
- Concentration
- Purification notes
Purified from bacteria by affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-HPV11 E2 antibody [10E12] (ab100968), expandable thumbnail](https://content.abcam.com/products/images/hpv11-e2-antibody-10e12-ab100968--western-blot-img15656.jpg "Western blot - Anti-HPV11 E2 antibody [10E12] (ab100968)")
Western blot - Anti-HPV11 E2 antibody [10E12] (ab100968)
All lanes: Western blot - Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution
Lanes 1 and 4: lysate of U20S cells transfected with plasmid expressing HPV11 E2 protein
Lane 2: lysate of plain U20S cells
Lane 3: lysate of plain HeLa cells
Lane 5: marker
Predicted band size: 42 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (ab100968), expandable thumbnail](https://content.abcam.com/products/images/hpv11-e2-antibody-10e12-ab100968--immunocytochemistry-immunofluorescence-img125062.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (ab100968)")
Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E2 antibody [10E12] (ab100968)
Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells.
IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.
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