Anti-HPV16 E7 antibody [EPR30642-21]

• WB

Supplier Data

Western blot - Anti-HPV16 E7 antibody [EPR30642-21] (AB325396)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

No cross-reactivity was observed with overexpressed HPV16 E6, HPV18 E7, HPV35 E7, HPV31 E7, HPV33 E7, HPV52 E7 and HPV58 E7 by Western Blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-HPV16 E7 antibody [EPR30642-21] (ab325396) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 2:

293T cells transfected with HPV16 E7 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 3:

293T cells transfected with HPV16 E6 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 4:

293T cells transfected with HPV18 E7 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 5:

293T cells transfected with HPV35 E7 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 6:

293T cells transfected with HPV31 E7 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 7:

293T cells transfected with HPV33 E7 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 8:

293T cells transfected with HPV52 E7 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 9:

293T cells transfected with HPV58 E7 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,36 kDa,15-20 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-HPV16 E7 antibody [EPR30642-21] (AB325396)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : 293T(PMID : 34729114); HeLa, U-2 OS(PMID : 20970819).

The blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The identity of the higher MW bands at approximately 37 kDa and 150KDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HPV16 E7 antibody [EPR30642-21] (ab325396) at 1/1000 dilution

Lane 1:

SiHa (human cervical epithelial cell) transfected with scrambled siRNA control whole cell lysate at 72 µg

Lane 2:

SiHa transfected with siRNA specifically targeting HPV16 E7 whole cell lysate at 72 µg

Lane 3:

Ca Ski (human Uterus Cervix epithelial) whole cell lysate at 72 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 72 µg

Lane 5:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 72 µg

Lane 6:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 72 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,36 kDa

false

Exposure time: 180s