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AB100969

Anti-HPV18 E2 antibody [2E7]

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(2 Publications)

Mouse Monoclonal VE2 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human papillomavirus samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human papillomavirus type 18 E2.

View Alternative Names

Regulatory protein E2, E2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (AB100969)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E2 antibody [2E7] (AB100969)

Immunofluorescence (IF) analysis of HPV18 E2 protein in U2OS cells. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E2 detected by ab100969.

Western blot - Anti-HPV18 E2 antibody [2E7] (AB100969)
  • WB

Unknown

Western blot - Anti-HPV18 E2 antibody [2E7] (AB100969)

All lanes:

Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969) at 1/100 dilution

Lane 1:

lysates of U20S cells transfected with plasmid expressing HPV11 E2 protein

Lane 2:

lysates of plain U20S cells

Lane 3:

lysates of plain HeLa cells

Lane 4:

lysates of U20S transfected with plasmid expressing HPV11 E2 protein

Lane 5:

marker

Predicted band size: 41 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2E7

Isotype

IgG

Carrier free

No

Reacts with

Human papillomavirus

Applications

ICC/IF, WB, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human papillomavirus type 18 E2.

P06790

Epitope

Mapped to amino acids 1-83.

Specificity

Reacts in WB, and ELISA with HPV18 native E2 protein as well recombinant E2 protein expressed in bacteria and Cos 7 cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "0.1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from bacteria by affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.
See full target information E2

Additional targets

HPV18 E2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Archives of virology 164:1815-1827 PubMed30631960

2019

Intratype variants of the E2 protein from human papillomavirus type 18 induce different gene expression profiles associated with apoptosis and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Alma Mariana Fuentes-González,J Omar Muñoz-Bello,Joaquín Manzo-Merino,Adriana Contreras-Paredes,Abraham Pedroza-Torres,Jorge Fernández-Retana,Carlos Pérez-Plasencia,Marcela Lizano

International journal of biological sciences 14:21-35 PubMed29483822

2018

Human Papillomavirus Types 16 and 18 Early-expressed Proteins Differentially Modulate the Cellular Redox State and DNA Damage.

Applications

WB

Species

Unspecified reactive species

Alfredo Cruz-Gregorio,Joaquín Manzo-Merino,María Cecilia Gonzaléz-García,José Pedraza-Chaverri,Omar Noel Medina-Campos,Mahara Valverde,Emilio Rojas,María Alexandra Rodríguez-Sastre,Claudia María García-Cuellar,Marcela Lizano
View all publications
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Product promise

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