Anti-HPV18 E7 antibody [8E2] (ab100953) is a mouse monoclonal antibody that is used to detect HPV18 E7 in Western Blot, IP, ICC/IF, ELISA. Suitable for Human papillomavirus samples.
- Over 10 publications
- Trusted since 2010
Images
![Western blot - Anti-HPV18 E7 antibody [8E2] (AB100953), expandable thumbnail](https://content.abcam.com/products/images/hpv18-e7-antibody-8e2-ab100953--western-blot-img79753.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HPV18 E7 antibody [8E2] (AB100953)")
![Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (AB100953), expandable thumbnail](https://content.abcam.com/products/images/hpv18-e7-antibody-8e2-ab100953--immunoprecipitation-img132206.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (AB100953)")
![Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (AB100953), expandable thumbnail](https://content.abcam.com/products/images/hpv18-e7-antibody-8e2-ab100953--immunocytochemistry-immunofluorescence-img74281.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (AB100953)")
Publications
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- Oncogene 42:2558-25772023Exploitation of ATP-sensitive potassium ion (K) channels by HPV promotes cervical cancer cell proliferation by contributing to MAPK/AP-1 signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames A Scarth,Christopher W Wasson,Molly R Patterson,Debra Evans,Diego Barba-Moreno,Holli Carden,Rosa Cassidy,Adrian Whitehouse,Jamel Mankouri,Adel Samson,Ethan L Morgan,Andrew MacdonaldPubMed 37443304
- Cell death and differentiation 28:1669-16872020E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEthan L Morgan,James A Scarth,Molly R Patterson,Christopher W Wasson,Georgia C Hemingway,Diego Barba-Moreno,Andrew MacdonaldPubMed 33303976
- International journal of molecular medicine 47:335-3452020Resveratrol inhibits the progression of cervical cancer by suppressing the transcription and expression of HPV E6 and E7 genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaodong Sun,Pan Fu,Lixia Xie,Senmao Chai,Qianqian Xu,Lian Zeng,Xuanbin Wang,Nan Jiang,Ming SangPubMed 33236130
- PLoS pathogens 16:e10086242020MicroRNA-18a targeting of the STK4/MST1 tumour suppressor is necessary for transformation in HPV positive cervical cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEthan L Morgan et. al.PubMed 32555725
- Antiviral research 178:1047942020In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYili Chen et. al.PubMed 32298665
- Cancers 11:2019JAK2 Inhibition Impairs Proliferation and Sensitises Cervical Cancer Cells to Cisplatin-Induced Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEthan L Morgan et. al.PubMed 31817106
- Cancer cell international 19:2412019Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Li et. al.PubMed 31572058
- PLoS biology 17:e30003672019Structural basis for recognition of the tumor suppressor protein PTPN14 by the oncoprotein E7 of human papillomavirus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye-Yeoung Yun,Min Wook Kim,Hye Seon Lee,Wantae Kim,Ji Hye Shin,Hyunmin Kim,Ho-Chul Shin,Hwangseo Park,Byung-Ha Oh,Won Kon Kim,Kwang-Hee Bae,Sang Chul Lee,Eun-Woo Lee,Bonsu Ku,Seung Jun KimPubMed 31323018
- Cell death and differentiation 26:1750-17652018A DHX9-lncRNA-MDM2 interaction regulates cell invasion and angiogenesis of cervical cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangya Ding,Xuemei Jia,Cong Wang,Jingyun Xu,Shou-Jiang Gao,Chun LuPubMed 30518908
- PLoS biology 16:e20057522018Disruption of CTCF-YY1-dependent looping of the human papillomavirus genome activates differentiation-induced viral oncogene transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIeisha Pentland et. al.PubMed 30359362
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human papillomavirus type 18 E7. Database link P06788
Reactivity data
Select an application| IP | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Human papillomavirus | Tested | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus | Dilution info 1/500 | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus | Dilution info 1/128000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus | Dilution info - | Notes - |
Target data
Function
Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta) (PubMed:26405230).
Additional Targets
HPV18 E7
Alternative names
Protein E7, E7
Recommended products
Anti-HPV18 E7 antibody [8E2] (ab100953) is a mouse monoclonal antibody that is used to detect HPV18 E7 in Western Blot, IP, ICC/IF, ELISA. Suitable for Human papillomavirus samples.
- Over 10 publications
- Trusted since 2010
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human papillomavirus type 18 E7. Database link P06788
- Clone number
- 8E2
- Purification technique
- Affinity purification Protein G
- Epitope
- Mapped to amino acids 36-70
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-HPV18 E7 antibody [8E2] (ab100953) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human papillomavirus samples.
What is the molecular weight of HPV18 E7?
Anti-HPV18 E7 [8E2] (ab100953) specifically detects a band for HPV18 E7 (UniProt: P06788) at a molecular weight of 12kDa.
Trusted by the scientific community
Anti-HPV18 E7 [8E2] (ab100953) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953), expandable thumbnail](https://content.abcam.com/products/images/hpv18-e7-antibody-8e2-ab100953--western-blot-img79753.jpg "Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)")
Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)
All lanes: Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953) at 1/1000 dilution
Lane 1: lysate of U20S cells with plasmid expressing E7 protein
Lane 2: plain U20S lysate
Lane 3: Hela lysate. HeLa cells containing HPV-18 genome sequences expressing HPV E6 and E7 proteins.
Lane 4: SiHa lysate
Lane 5: Marker
Predicted band size: 12 kDa
![Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953), expandable thumbnail](https://content.abcam.com/products/images/hpv18-e7-antibody-8e2-ab100953--immunoprecipitation-img132206.jpg "Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)")
Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)
IP analysis of HPV18 E7 protein using ab100953 at 1/500:
Lane 1: detection of E7 protein in lysate of U20S cells
Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of antibody
Lane 3: precipitation without ab100953
Lane 4: IP exp. with plain U2OS cells
Lane 5: IP exp. with non-transfected HeLa cells. Lower band corresponds to native HPV-18 E7 protein expressed by HPV-18 genome expressed in Hela cells.
Lane 6: marker.All lanes: Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)
Predicted band size: 12 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953), expandable thumbnail](https://content.abcam.com/products/images/hpv18-e7-antibody-8e2-ab100953--immunocytochemistry-immunofluorescence-img74281.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)")
Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)
Immunofluorescent (IF) analysis of HPV18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E7 detected by ab100953. IF analysis of E7 protein shows similar result also in HeLa cells.
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