AB100953

Anti-HPV18 E7 antibody [8E2]

Name: Anti-HPV18 E7 antibody [8E2] (ab100953) | Abcam
Brand: Abcam Limited
SKU: AB100953
Rating: 4 (1 reviews)

(1 Review)

(26 Publications)

Anti-HPV18 E7 antibody [8E2] (ab100953) is a mouse monoclonal antibody detecting HPV18 E7 in Western Blot, IP, ICC/IF, ELISA. Suitable for Human papillomavirus.

- Over 10 publications
- Trusted since 2010

View Alternative Names

Protein E7, E7, E7 protein, Human Papilloma Virus Type 18 E7 protein

3 Images

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Western blot - Anti-HPV18 E7 antibody [8E2] (AB100953)

All lanes:

Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953) at 1/1000 dilution

Lane 1:

lysate of U20S cells with plasmid expressing E7 protein

Lane 2:

plain U20S lysate

Lane 3:

Hela lysate. HeLa cells containing HPV-18 genome sequences expressing HPV E6 and E7 proteins.

Lane 4:

SiHa lysate

Lane 5:

Marker

Predicted band size: 12 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (AB100953)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (AB100953)

Immunofluorescent (IF) analysis of HPV18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E7 detected by ab100953. IF analysis of E7 protein shows similar result also in HeLa cells.

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Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (AB100953)

IP analysis of HPV18 E7 protein using ab100953 at 1/500 :
Lane 1 : detection of E7 protein in lysate of U20S cells
Lane 2 : the lower band is precipitated E7, upper bands are light and heavy chain of antibody
Lane 3 : precipitation without ab100953
Lane 4 : IP exp. with plain U2OS cells
Lane 5 : IP exp. with non-transfected HeLa cells. Lower band corresponds to native HPV-18 E7 protein expressed by HPV-18 genome expressed in Hela cells.
Lane 6 : marker.

All lanes:

Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)

Predicted band size: 12 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8E2

Isotype

IgG1

Carrier free

Reacts with

Human papillomavirus

Applications

WB, ICC/IF, ELISA, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Human papillomavirus type 18 E7.

P06788

Epitope

Mapped to amino acids 36-70

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/500", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/128000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-HPV18 E7 antibody [8E2] (ab100953) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human papillomavirus samples.

What is the molecular weight of HPV18 E7?
Anti-HPV18 E7 [8E2] (ab100953) specifically detects a band for HPV18 E7 (UniProt: P06788) at a molecular weight of 12kDa.

Trusted by the scientific community
Anti-HPV18 E7 [8E2] (ab100953) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta) (PubMed : 26405230).

See full target information E7

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:3041 PubMed39856147

2025

A paper-based HPV E7 oncoprotein assay for cervical precancer detection at the point of care.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsey A Smith,Sai Paul,Karen E Haney,Sonia G Parra,Meaghan Bond,Leticia López,Mauricio Maza,Juan Felix,Preetha Ramalingam,Pablo Escobar,Philip E Castle,Kathleen M Schmeler,Rebecca R Richards-Kortum

Biotechnology and applied biochemistry 72:104-115 PubMed39113388

2024

HPV18 E6/E7 activates Ca influx to promote the malignant progression of cervical cancer by inhibiting Ca binding protein 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Kang,Lei Qiu,Yali Duo,FengLing Bi,Zhongjie Liu,Jing Wang,Lei Zheng,Ning Zhao

Nature communications 15:5809 PubMed38987584

2024

The Hippo pathway transcription factors YAP and TAZ play HPV-type dependent roles in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Molly R Patterson,Joseph A Cogan,Rosa Cassidy,Daisy A Theobald,Miao Wang,James A Scarth,Chinedu A Anene,Adrian Whitehouse,Ethan L Morgan,Andrew Macdonald

Oncogene 43:2184-2198 PubMed38789663

2024

E7-mediated repression of miR-203 promotes LASP1-dependent proliferation in HPV-positive cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Molly R Patterson,Aniek S Meijers,Emma L Ryder,Louisa M Wootton,James A Scarth,Debra Evans,Amy L Turner,Christopher W Wasson,Janne E Darell,Daisy A Theobald,Joseph A Cogan,Claire D James,Miao Wang,John E Ladbury,Iain M Morgan,Adel Samson,Ethan L Morgan,Andrew Macdonald

Nature communications 15:2271 PubMed38480731

2024

HLA-class II restricted TCR targeting human papillomavirus type 18 E7 induces solid tumor remission in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianting Long,Xihe Chen,Mian He,Shudan Ou,Yunhe Zhao,Qingjia Yan,Minjun Ma,Jingyu Chen,Xuping Qin,Xiangjun Zhou,Junjun Chu,Yanyan Han

Journal of medical virology 95:e29025 PubMed37565725

2023

CREB1 activation promotes human papillomavirus oncogene expression and cervical cancer cell transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Yigen Li,Molly R Patterson,Ethan L Morgan,Christopher W Wasson,Emma L Ryder,Diego Barba-Moreno,James A Scarth,Miao Wang,Andrew Macdonald

Oncogene 42:2558-2577 PubMed37443304

2023

Exploitation of ATP-sensitive potassium ion (K) channels by HPV promotes cervical cancer cell proliferation by contributing to MAPK/AP-1 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

James A Scarth,Christopher W Wasson,Molly R Patterson,Debra Evans,Diego Barba-Moreno,Holli Carden,Rosa Cassidy,Adrian Whitehouse,Jamel Mankouri,Adel Samson,Ethan L Morgan,Andrew Macdonald

Cell biology international 47:1327-1343 PubMed37191290

2023

HPV E6 promotes cell proliferation of cervical cancer cell by accelerating accumulation of RBM15 dependently of autophagy inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Nie,Bo Tang,Mingfen Lv,Danyang Li,Tian Li,Rongying Ou,Yunsheng Xu,Juan Wen

Cancer medicine 11:1249-1268 PubMed34994088

2022

Nocardia rubra cell-wall skeleton influences the development of cervical carcinoma by promoting the antitumor effect of macrophages and dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Siyang Zhang,Han Wang,Yisi Liu,Tao Tao,Zhi Zeng,Yingying Zhou,Min Wang

Cell death and differentiation 28:1669-1687 PubMed33303976

2020

E6-mediated activation of JNK drives EGFR signalling to promote proliferation and viral oncoprotein expression in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ethan L Morgan,James A Scarth,Molly R Patterson,Christopher W Wasson,Georgia C Hemingway,Diego Barba-Moreno,Andrew Macdonald

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HPV18 E7 antibody [8E2]

Western blot - Anti-HPV18 E7 antibody [8E2] (AB100953)

Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (AB100953)

Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (AB100953)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (26)

Product promise