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Rabbit Recombinant Monoclonal VE7 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human papillomavirus type 18, Transfected cell lysate - Human papillomavirus type 18 samples.

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Images

Flow Cytometry (Intracellular) - Anti-HPV18 E7 antibody [EPR26911-29] (AB308296), expandable thumbnail
  • Immunoprecipitation - Anti-HPV18 E7 antibody [EPR26911-29] (AB308296), expandable thumbnail
  • Western blot - Anti-HPV18 E7 antibody [EPR26911-29] (AB308296), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [EPR26911-29] (AB308296), expandable thumbnail
  • Western blot - Anti-HPV18 E7 antibody [EPR26911-29] (AB308296), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IP
Human papillomavirus type 18
Tested
Tested
Tested
Tested
Transfected cell lysate - Human papillomavirus type 18
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human papillomavirus type 18

Dilution info

1/1000

Notes

-

Species

Transfected cell lysate - Human papillomavirus type 18

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human papillomavirus type 18

Dilution info

1/100

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human papillomavirus type 18

Dilution info

-

Notes

-

Tested
Tested

Species

Human papillomavirus type 18

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human papillomavirus type 18

Dilution info

-

Notes

-

Tested
Tested

Species

Human papillomavirus type 18

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human papillomavirus type 18

Dilution info

-

Notes

-

Target data

Function

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta) (PubMed:26405230).

Alternative names

Protein E7, E7

Recommended products

Rabbit Recombinant Monoclonal VE7 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human papillomavirus type 18, Transfected cell lysate - Human papillomavirus type 18 samples.

Alternative names

Protein E7, E7

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26911-29

Purification technique

Affinity purification Protein A

Specificity

Cross-reactivity was observed with overexpressed HPV45 E7 protein by western blot and flow cytometry. No cross-reactivity was observed with overexpressed HPV70 E7, HPV39 E7, HPV16 E7 and HPV18 E6.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry (Intracellular) - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype control / HEK-293T cells transfected with Myc-tagged HPV18 Protein E7 overexpression construct / HPV45 Protein E7 overexpression construct / HPV70 Protein E7 overexpression construct / HPV39 Protein E7 overexpression construct / HPV16 Protein E7 overexpression construct / HPV18 Protein E6 overexpression construct labelling HPV18 E7 with ab308296 at 1/500 dilution (0.1 ug).
    A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296), expandable thumbnail

    Immunoprecipitation - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296)

    HPV18 E7 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab308296 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308296 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 ug

    Lane 2: ab308296 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab308296 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    All lanes: Immunoprecipitation - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296) at 1/30 dilution

    Lanes 1 - 2: ab308296 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab308296 in HeLa whole cell lysate

    Observed band size: 17 kDa

  • Western blot - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296), expandable thumbnail

    Western blot - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Weak cross-reactivity was observed with HPV45 E7 protein.

    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296) at 1/1000 dilution

    Lane 1: 293T cells transfected with a HPV18 E7 expression vector containing a His-tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a HPV45 E7 expression vector containing a His-tag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a HPV39 E7 expression vector containing a His-tag whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a HPV16 E7 expression vector containing a His-tag whole cell lysate at 20 µg

    Lane 5: 293T cells transfected with a HPV18 E6 expression vector containing a His-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling HPV18 E7 with ab308296 at 1/100 dilution (5.09 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).

    Confocal image showing cytoplasmic and nuclear staining in HeLa cell line.

    Negative control: U-2 OS (PMID: 22135254).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

  • Western blot - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296), expandable thumbnail

    Western blot - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: U-2 OS (PMID:22135254), human testis, human stomach.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.

    Exposure time: 48 seconds.

    All lanes: Western blot - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 20 µg

    Lane 2: U-2 OS whole cell lysate at 20 µg

    Lane 3: Human testis tissue lysate at 20 µg

    Lane 4: Human stomach tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa

  • Flow Cytometry (Intracellular) - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HPV18 E7 antibody [EPR26911-29] (ab308296)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-2 OS (human bone osteosarcoma epithelial cell) (Left panel) compared to HeLa (human cervical adenocarcinoma epithelial cell) (Right panel) cells labelling HPV18 E7 with ab308296 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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