Rabbit Recombinant Monoclonal RASH antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (AB240323), expandable thumbnail](https://content.abcam.com/products/images/hras-kras-antibody-epr18713-bsa-and-azide-free-ab240323--immunoprecipitation-img10724.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (AB240323)")
![Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (AB240323), expandable thumbnail](https://content.abcam.com/products/images/hras-kras-antibody-epr18713-bsa-and-azide-free-ab240323--immunocytochemistry-immunofluorescence-img65756.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (AB240323)")
![Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (AB240323), expandable thumbnail](https://content.abcam.com/products/images/hras-kras-antibody-epr18713-bsa-and-azide-free-ab240323--immunocytochemistry-immunofluorescence-img22872.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (AB240323)")
Publications
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- iScience 26:1057232023Assessment of stromal SCD-induced drug resistance of PDAC using 3D-printed zPDX model chips.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuntao Wu,Beiyuan Hu,Lei Wang,Xia Wu,Haitao Gu,Hanguang Dong,Jiuliang Yan,Zihao Qi,Qi Zhang,Huan Chen,Bo Yu,Sheng Hu,Yu Qian,Shuang Dong,Qiang Li,Xu Wang,Jiang LongPubMed 36590169
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).
Additional Targets
KRAS
Alternative names
HRAS1, HRAS, GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras
Recommended products
Rabbit Recombinant Monoclonal RASH antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18713
- Purification technique
- Affinity purification Protein A
- Specificity
Immunoprecipitation requires a mild lysis buffer to achieve an acceptable enrichment.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240323 is the carrier-free version of Anti-HRAS + KRAS antibody [EPR18713] ab191595.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323), expandable thumbnail](https://content.abcam.com/products/images/hras-kras-antibody-epr18713-bsa-and-azide-free-ab240323--immunoprecipitation-img10724.jpg "Immunoprecipitation - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323)")
Immunoprecipitation - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323)
KRAS was immunoprecipitated from 1mg of A549 (Human lung carcinoma) whole cell lysate with Anti-HRAS + KRAS antibody [EPR18713] ab191595 at 1/70 dilution.
Western blot was performed from the immunoprecipitate using Anti-HRAS + KRAS antibody [EPR18713] ab191595 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: A549 whole cell lysate 10ug (Input).
Lane 2: Anti-HRAS + KRAS antibody [EPR18713] ab191595 IP in A549 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-HRAS + KRAS antibody [EPR18713] ab191595 in A549 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HRAS + KRAS antibody [EPR18713] ab191595).
All lanes: Immunoprecipitation - Anti-HRAS + KRAS antibody [EPR18713] (Anti-HRAS + KRAS antibody [EPR18713] ab191595)
![Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323), expandable thumbnail](https://content.abcam.com/products/images/hras-kras-antibody-epr18713-bsa-and-azide-free-ab240323--immunocytochemistry-immunofluorescence-img65756.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323)")
Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KRAS + HRAS with Anti-HRAS + KRAS antibody [EPR18713] ab191595 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasm and nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-HRAS + KRAS antibody [EPR18713] ab191595 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HRAS + KRAS antibody [EPR18713] ab191595).
![Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323), expandable thumbnail](https://content.abcam.com/products/images/hras-kras-antibody-epr18713-bsa-and-azide-free-ab240323--immunocytochemistry-immunofluorescence-img22872.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323)")
Immunocytochemistry/ Immunofluorescence - Anti-HRAS + KRAS antibody [EPR18713] - BSA and Azide free (ab240323)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling KRAS + HRAS with Anti-HRAS + KRAS antibody [EPR18713] ab191595 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasm and nuclear staining on NIH/3T3 cells line.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-HRAS + KRAS antibody [EPR18713] ab191595 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HRAS + KRAS antibody [EPR18713] ab191595).
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