Anti-HRH1 antibody [HL3037] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-HRH1 antibody [HL3037] - BSA and Azide free (AB325864)

Untreated (–) and treated (+) unboiled MDA-MB-231 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325864 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-HRH1 antibody [HL3037] - BSA and Azide free (ab325864) at 1/1000 dilution

Lane 1:

Untreated (–) unboiled MDA-MB-231 whole cell extracts at 30 µg

Lane 2:

PNGase F, 2 hr treated (+) unboiled MDA-MB-231 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HRH1 antibody [HL3037] - BSA and Azide free (AB325864)

ab325864 detects HRH1 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : HRH1 stained by ab325864 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-HRH1 antibody [HL3037] - BSA and Azide free (AB325864)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325864 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-HRH1 antibody [HL3037] - BSA and Azide free (ab325864) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged HRH1 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged HRH1 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-HRH1 antibody [HL3037] - BSA and Azide free (AB325864)

Non-transfected (–) and transfected (+) unboiled 5637 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325864 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-HRH1 antibody [HL3037] - BSA and Azide free (ab325864) at 1/1000 dilution

Lane 1:

Non-transfected (–) unboiled 5637 whole cell extracts at 30 µg

Lane 2:

HRH1 shRNA transfected (+) unboiled 5637 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false