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AB45419

Anti-Hrk antibody

3

(1 Review)

|

(6 Publications)

Rabbit Polyclonal HRK antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human HRK.

View Alternative Names

BID3, HRK, Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3, Neuronal death protein DP5

2 Images
Western blot - Anti-Hrk antibody (AB45419)
  • WB

Unknown

Western blot - Anti-Hrk antibody (AB45419)

Lane 1:

Western blot - Anti-Hrk antibody (ab45419) at 2.5 µg/mL

Lane 2:

Western blot - Anti-Hrk antibody (ab45419) at 5 µg/mL

All lanes:

Mouse pancreas tissue lysate

Predicted band size: 10 kDa

Observed band size: 13 kDa

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Western blot - Anti-Hrk antibody (AB45419)
  • WB

CiteAb

Western blot - Anti-Hrk antibody (AB45419)

Hrk western blot using anti-Hrk antibody ab45419. Publication image and figure legend from Duval, N., Sumner, W. A., et al., 2018, Front Cell Neurosci, PubMed 29440992.

ab45419 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab45419 please see the product overview.

Western blot analysis of Hrk and BNip3L expression in WT and G93A mutant SOD1 lumbar spinal cord tissue. (A) Hrk protein expression in 3 WT and 5 G93A mutant (M) SOD1 animals. COX-IV serves as a loading control. (B) Densitometry of Hrk bands normalized to COX-IV loading control and analyzed by unpaired t-test : WT versus G93A, *p < 0.05. (C) BNip3L protein expression in three WT and three G93A mutant SOD1 animals. β-tubulin serves as a loading control. (D) Densitometry of BNip3L bands normalized to β-tubulin loading control, and analyzed by unpaired t-test : WT versus G93A, **p < 0.01. WT, wild type mouse; M, SOD1 mutant mouse.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human HRK. The exact immunogen used to generate this antibody is proprietary information.

O00198

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2.5-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hrk also known as Harakiri is a pro-apoptotic protein that has a molecular mass of about 13 kDa. The protein has an important role in apoptosis by promoting cell death through interaction with anti-apoptotic members of the Bcl-2 protein family. Hrk localizes mainly in the outer mitochondrial membrane where it is highly expressed in cells that are undergoing apoptosis especially in neural and hematopoietic tissues.
Biological function summary

Hrk contributes to the induction of apoptosis by binding directly to the Bcl-2 family members Bcl-xL and Bcl-2 inhibiting their anti-apoptotic functions. Through this interaction Hrk facilitates mitochondrial cytochrome c release an essential step in the apoptotic cascade. Hrk does not form part of a larger complex but acts individually to promote cell death particularly in pathways concerning developmental and stress-induced apoptosis.

Pathways

Hrk plays a critical role in the intrinsic apoptosis pathway. It triggers downstream apoptotic events by enhancing mitochondrial outer membrane permeabilization which recruits relevant proteins like bax and bak to amplify the apoptotic signal. Hrk’s interaction in the apoptosis pathway also connects it closely with the mitochondrial release of cytochrome c which later activates caspases leading to cell death.

Hrk has a significant involvement in conditions like neurodegenerative diseases and cancer. Abnormal regulation of Hrk expression links to diseases such as Alzheimer's where it may contribute to excessive neuronal apoptosis. Similarly cancer cells may evade apoptosis by down-regulating Hrk expression. Hrk's interaction with proteins like Bcl-2 and Bcl-xL highlights its possible role in therapeutic targets given Bcl-2's association with various cancers.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Promotes apoptosis.
See full target information HRK

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:194 PubMed40113795

2025

Novel selective strategies targeting the BCL-2 family to enhance clinical efficacy in ALK-rearranged non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Martín,Clara Alcon,Elba Marín,Paula Morales-Sánchez,Albert Manzano-Muñoz,Sherley Díaz,Mireia García,Josep Samitier,Albert Lu,Alberto Villanueva,Noemí Reguart,Cristina Teixido,Joan Montero

Oncogene 41:5046-5060 PubMed36241868

2022

AKT-mTORC1 reactivation is the dominant resistance driver for PI3Kβ/AKT inhibitors in PTEN-null breast cancer and can be overcome by combining with Mcl-1 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Shanade Dunn,Cath Eberlein,Jason Yu,Albert Gris-Oliver,Swee Hoe Ong,Urs Yelland,Natalie Cureton,Anna Staniszewska,Robert McEwen,Millie Fox,James Pilling,Philip Hopcroft,Elizabeth A Coker,Patricia Jaaks,Mathew J Garnett,Beverley Isherwood,Violeta Serra,Barry R Davies,Simon T Barry,James T Lynch,Kosuke Yusa

Cancers 13: PubMed34572804

2021

The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liang,Mingo M H Yung,Fangfang He,Peili Jiao,Karen K L Chan,Hextan Y S Ngan,David W Chan

Nature communications 9:2829 PubMed30026490

2018

Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Huajie Song,Hong Mei,Erhu Fang,Xiaojing Wang,Feng Yang,Huanhuan Li,Yajun Chen,Kai Huang,Liduan Zheng,Qiangsong Tong

Frontiers in cellular neuroscience 12:15 PubMed29440992

2018

The Bcl-2 Homology-3 Domain (BH3)-Only Proteins, Bid, DP5/Hrk, and BNip3L, Are Upregulated in Reactive Astrocytes of End-Stage Mutant SOD1 Mouse Spinal Cord.

Applications

WB

Species

Mouse

Nathan Duval,Whitney A Sumner,Anna G Andrianakos,Josie J Gray,Ron J Bouchard,Heather M Wilkins,Daniel A Linseman

International journal of molecular sciences 16:1266-92 PubMed25574601

2015

Molecular targeting of the oncoprotein PLK1 in pediatric acute myeloid leukemia: RO3280, a novel PLK1 inhibitor, induces apoptosis in leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Na-Na Wang,Zhi-Heng Li,He Zhao,Yan-Fang Tao,Li-Xiao Xu,Jun Lu,Lan Cao,Xiao-Juan Du,Li-Chao Sun,Wen-Li Zhao,Pei-Fang Xiao,Fang Fang,Guang-Hao Su,Yan-Hong Li,Gang Li,Yi-Ping Li,Yun-Yun Xu,Hui-Ting Zhou,Yi Wu,Mei-Fang Jin,Lin Liu,Jian Ni,Jian Wang,Shao-Yan Hu,Xue-Ming Zhu,Xing Feng,Jian Pan
View all publications

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