Anti-Hrk antibody
3
(1 Review)
|
(6 Publications)
Rabbit Polyclonal HRK antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human HRK.
View Alternative Names
BID3, HRK, Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3, Neuronal death protein DP5
- WB
Unknown
Western blot - Anti-Hrk antibody (AB45419)
Lane 1:
Western blot - Anti-Hrk antibody (ab45419) at 2.5 µg/mL
Lane 2:
Western blot - Anti-Hrk antibody (ab45419) at 5 µg/mL
All lanes:
Mouse pancreas tissue lysate
Predicted band size: 10 kDa
Observed band size: 13 kDa
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- WB
CiteAb
Western blot - Anti-Hrk antibody (AB45419)
Hrk western blot using anti-Hrk antibody ab45419. Publication image and figure legend from Duval, N., Sumner, W. A., et al., 2018, Front Cell Neurosci, PubMed 29440992.
ab45419 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab45419 please see the product overview.
Western blot analysis of Hrk and BNip3L expression in WT and G93A mutant SOD1 lumbar spinal cord tissue. (A) Hrk protein expression in 3 WT and 5 G93A mutant (M) SOD1 animals. COX-IV serves as a loading control. (B) Densitometry of Hrk bands normalized to COX-IV loading control and analyzed by unpaired t-test : WT versus G93A, *p < 0.05. (C) BNip3L protein expression in three WT and three G93A mutant SOD1 animals. β-tubulin serves as a loading control. (D) Densitometry of BNip3L bands normalized to β-tubulin loading control, and analyzed by unpaired t-test : WT versus G93A, **p < 0.01. WT, wild type mouse; M, SOD1 mutant mouse.
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Reactivity data
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Supplementary information
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Biological function summary
Hrk contributes to the induction of apoptosis by binding directly to the Bcl-2 family members Bcl-xL and Bcl-2 inhibiting their anti-apoptotic functions. Through this interaction Hrk facilitates mitochondrial cytochrome c release an essential step in the apoptotic cascade. Hrk does not form part of a larger complex but acts individually to promote cell death particularly in pathways concerning developmental and stress-induced apoptosis.
Pathways
Hrk plays a critical role in the intrinsic apoptosis pathway. It triggers downstream apoptotic events by enhancing mitochondrial outer membrane permeabilization which recruits relevant proteins like bax and bak to amplify the apoptotic signal. Hrk’s interaction in the apoptosis pathway also connects it closely with the mitochondrial release of cytochrome c which later activates caspases leading to cell death.
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Publications (6)
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Cell death & disease 16:194 PubMed40113795
2025
Applications
Unspecified application
Species
Unspecified reactive species
Oncogene 41:5046-5060 PubMed36241868
2022
Applications
Unspecified application
Species
Unspecified reactive species
Cancers 13: PubMed34572804
2021
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 9:2829 PubMed30026490
2018
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in cellular neuroscience 12:15 PubMed29440992
2018
Applications
WB
Species
Mouse
International journal of molecular sciences 16:1266-92 PubMed25574601
2015
Applications
Unspecified application
Species
Unspecified reactive species
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