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AB199523

HRP Anti-4-1BBL antibody [EPR1172Y]

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(1 Publication)

Rabbit Recombinant Monoclonal 4-1BBL antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, TNFSF9

1 Images
Western blot - HRP Anti-4-1BBL antibody [EPR1172Y] (AB199523)
  • WB

Lab

Western blot - HRP Anti-4-1BBL antibody [EPR1172Y] (AB199523)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199523 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-4-1BBL antibody [EPR1172Y] (ab199523) at 1/5000 dilution

All lanes:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Predicted band size: 27 kDa

Observed band size: 30 kDa

true

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1172Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 4-1BBL protein also known as CD137L or 4-1BB ligand acts mechanically as a member of the tumor necrosis factor (TNF) superfamily. The 4-1BBL with a mass of around 36 kDa exists as a type II transmembrane protein. It gets expressed on activated antigen-presenting cells like dendritic cells macrophages and B cells. This costimulatory molecule primarily interacts with its receptor 4-1BB (CD137) on T cells promoting their activation.
Biological function summary

The 4-1BBL protein plays an important role in mediating the co-stimulatory signal needed for T-cell proliferation survival and cytokine production. This protein contributes to the formation of the immunological synapse and doesn't exist alone but rather interacts directly with its receptor CD137 forming a receptor-ligand complex. The 4-1BBL-CD137 interaction strengthens signals delivered by the T-cell receptor enhancing immune responses.

Pathways

The 4-1BBL protein integrates into immune-related signaling pathways. It significantly impacts the NF-kB and MAPK pathways important for T cell survival and activation. The interaction between 4-1BBL and its related proteins such as the 4-1BB/CD137 receptor initiates downstream cascades that lead to the activation of transcription factors involved in immune response.

The 4-1BBL protein connects to conditions such as cancer and autoimmune diseases. Overexpression or dysregulation of 4-1BBL has associations with autoimmune conditions like rheumatoid arthritis due to prolonged T cell activation. In cancer 4-1BBL's interaction with CD137 has potential therapeutic implications with efforts to harness this axis to bolster anti-tumor immunity. The modulation of 4-1BBL activity can influence disease outcomes highlighting its significance as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
See full target information TNFSF9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eade2819 PubMed37083528

2023

Cascaded microfluidic circuits for pulsatile filtration of extracellular vesicles from whole blood for early cancer diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenglin Li,Chao Liu,Yangchang Cheng,Yike Li,Jinqi Deng,Lixiao Bai,Lili Qin,Huili Mei,Min Zeng,Fei Tian,Shaohua Zhang,Jiashu Sun
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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