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AB202001

HRP Anti-58K Golgi protein antibody [58K-9] - Golgi Marker

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(1 Publication)

Mouse Monoclonal 58K Golgi protein antibody - conjugated to HRP. Golgi marker. Suitable for WB and reacts with Rat samples. Cited in 1 publication.

View Alternative Names

Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, LCHC1, FTCD

1 Images
Western blot - HRP Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (AB202001)
  • WB

Lab

Western blot - HRP Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (AB202001)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202001 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab202001) at 1/5000 dilution

All lanes:

Liver (Rat) Tissue Lysate at 10 µg

Predicted band size: 58 kDa

Observed band size: 58 kDa

true

Exposure time: 30s

  • Unconjugated

    Anti-58K Golgi protein antibody [58K-9] - Golgi Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

58K-9

Isotype

IgG1

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 58K Golgi protein also known as Golgi marker protein is a significant component within the Golgi apparatus with a molecular mass of approximately 58 kilodaltons (kDa). This protein is widely expressed in eukaryotic cells and serves as an important marker for studying the structure and function of the Golgi apparatus. The 58K Golgi protein localizes predominantly to the membranes of the Golgi where it plays an integral role in the maintenance of its architecture and function. Researchers often use this protein in Golgi immunofluorescence studies to visualize and track Golgi dynamics and morphology.
Biological function summary

The 58K Golgi protein contributes to the Golgi's role in modifying sorting and packaging proteins and lipids for secretion or delivery to other organelles. It does not function alone but often interacts with other Golgi markers and proteins forming part of a complex that helps regulate intracellular transport processes. These interactions are important for maintaining Golgi function and cellular protein distribution. By acting as a Golgi biomarker the 58K Golgi protein aids in understanding the trafficking and processing pathways essential for cellular function.

Pathways

The 58K Golgi protein is involved in the vesicular transport pathway which is essential for trafficking proteins between the endoplasmic reticulum (ER) and the Golgi apparatus. It interacts with other proteins like COPI and COPII which are critical for vesicle formation and movement through this pathway. This protein also plays a role in the glycosylation pathway where nascent proteins undergo carbohydrate modification in the Golgi. Such modifications assist in protein folding stability and cell signaling. The integration of 58K Golgi protein in these pathways highlights its importance in sustaining cellular operations.

The 58K Golgi protein has correlations with some pathologies associated with Golgi dysfunction including congenital disorders of glycosylation and neurodegenerative diseases such as Alzheimer's disease. Disruptions in Golgi structure and function can lead to improper protein sorting and trafficking which is observed in these disorders. Proteins such as amyloid precursor protein (APP) in Alzheimer's are impacted by disruptions in the Golgi pathway where 58K Golgi protein plays a role. Understanding these connections can help in developing therapeutic strategies targeting Golgi-associated dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.. Binds and promotes bundling of vimentin filaments originating from the Golgi.
See full target information FTCD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e30526 PubMed38737268

2024

Changes in transcriptomic landscape with macronutrients intake switch are independent from O-GlcNAcylation levels in heart throughout postnatal development in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Persello,Thomas Dupas,Amandine Vergnaud,Angélique Blangy-Letheule,Virginie Aillerie,Angélique Erraud,Yannick Guilloux,Manon Denis,Benjamin Lauzier
View all publications
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Product promise

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