AB197712

HRP Anti-67kDa Laminin Receptor antibody [EPR8469]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal 67kDa Laminin Receptor antibody - conjugated to HRP. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

LAMBR, LAMR1, RPSA, Small ribosomal subunit protein uS2, 37 kDa laminin receptor precursor, 37/67 kDa laminin receptor, 40S ribosomal protein SA, 67 kDa laminin receptor, Colon carcinoma laminin-binding protein, Laminin receptor 1, Laminin-binding protein precursor p40, Multidrug resistance-associated protein MGr1-Ag, NEM/1CHD4, 37LRP, LRP/LR, 67LR, LamR, LBP/p40

1 Images

Lab

Western blot - HRP Anti-67kDa Laminin Receptor antibody [EPR8469] (AB197712)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197712 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-67kDa Laminin Receptor antibody [EPR8469] (ab197712) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (<a href='/en-us/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Predicted band size: 33 kDa

Observed band size: 37 kDa

true

Exposure time: 12s

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-67kDa Laminin Receptor antibody [EPR8469]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-67kDa Laminin Receptor antibody [EPR8469]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-67kDa Laminin Receptor antibody [EPR8469]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-67kDa Laminin Receptor antibody [EPR8469]
578 PE

PE Anti-67kDa Laminin Receptor antibody [EPR8469]
660 APC

APC Anti-67kDa Laminin Receptor antibody [EPR8469]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-67kDa Laminin Receptor antibody [EPR8469]
Unconjugated

Anti-67kDa Laminin Receptor antibody [EPR8469]
Carrier free

Anti-67kDa Laminin Receptor antibody [EPR8469] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8469

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 67kDa Laminin Receptor also known as the laminin binding protein or protein laminin functions as a receptor for laminin an important component of the extracellular matrix. This receptor is expressed in many tissue types serving an important mechanical role by mediating the adhesion and migration of cells. The receptor anchors cells to laminin molecules thereby influencing cell differentiation and proliferation. The molecular weight of the laminin receptor is approximately 67 kilodaltons which signifies it as a critical player in various cellular processes.

Biological function summary

This receptor engages in cellular interactions that are essential for maintaining the integrity and architecture of tissues. It often associates with other proteins to form complexes which further strengthen its functional capabilities. By anchoring laminin to the cell surface it regulates cell signaling pathways that influence cell motility invasion and survival. These interactions suggest that the 67kDa Laminin Receptor's function goes beyond simple adhesion impacting cellular responsiveness to external matrix cues.

Pathways

The receptor influences the MAPK and PI3K/Akt signaling pathways which are significant in cell survival and proliferation. Through such pathways it interacts with numerous proteins including integrins and fibronectin receptors facilitating orchestrated cell signaling. This connectivity highlights the receptor's role in cellular communication networks contributing to dynamic processes like wound healing and angiogenesis.

Aberrant expression of the 67kDa Laminin Receptor relates to conditions such as cancer and Alzheimer's disease. In cancer its overexpression links to increased tumor invasiveness likely due to enhanced cell motility mediated through integrin interactions. In Alzheimer's disease its relationship with amyloid precursor protein influences plaque formation exacerbating disease progression. Understanding these connections highlights potential therapeutic targets for disease intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.. (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9.. (Microbial infection) Acts as a receptor for the Dengue virus.. (Microbial infection) Acts as a receptor for the Sindbis virus.. (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus.. (Microbial infection) Acts as a receptor for the pathogenic prion protein.. (Microbial infection) Acts as a receptor for bacteria.

See full target information RPSA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed39408267

2024

The Influence of Strain and Sex on High Fat Diet-Associated Alterations of Dopamine Neurochemistry in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen A Hagarty-Waite,Heather A Emmons,Steve C Fordahl,Keith M Erikson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-67kDa Laminin Receptor antibody [EPR8469]

Western blot - HRP Anti-67kDa Laminin Receptor antibody [EPR8469] (AB197712)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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