AB202004

HRP Anti-6X His tag® antibody [4D11]

Name: Anti-6X His tag® HRP antibody [4D11] (ab202004) IgG2a | Abcam
Brand: Abcam Limited
SKU: AB202004
Price: 590 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal 6X His tag® antibody - conjugated to HRP. Suitable for WB and reacts with Tag samples. Cited in 2 publications.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Lab

Western blot - HRP Anti-6X His tag® antibody [4D11] (AB202004)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202004 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-6X His tag® antibody [4D11] (ab202004) at 1/5000 dilution

All lanes:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (<a href='/en-us/products/cell-lysates/e-coli-positive-control-escherichia-coli-whole-cell-lysate-ab5395'>ab5395</a>) at 0.1 µg

true

Exposure time: 3min

Unconjugated

Anti-6X His tag® antibody [4D11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4D11

Isotype

IgG2a

Light chain type

kappa

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum :e0204324 PubMed39436135

2024

Development and optimization of a double antigen sandwich ELISA detecting for Senecavirus A antibodies based on VP2 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Zhengwang Shi,Juncong Luo,Caixia Jia,Xiaoyang Zhang,Juanjuan Wei,Shuaipeng Li,Yuqian Zhu,Tao Xi,Jing Zhou,Yindi He,Xintai Shi,Huanchen Liao,Hong Tian,Haixue Zheng

Heliyon 10:e30526 PubMed38737268

2024

Changes in transcriptomic landscape with macronutrients intake switch are independent from O-GlcNAcylation levels in heart throughout postnatal development in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Persello,Thomas Dupas,Amandine Vergnaud,Angélique Blangy-Letheule,Virginie Aillerie,Angélique Erraud,Yannick Guilloux,Manon Denis,Benjamin Lauzier

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-6X His tag® antibody [4D11]

Western blot - HRP Anti-6X His tag® antibody [4D11] (AB202004)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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