Anti-6X His tag® HRP conjugated rabbit polyclonal antibody that is used to detect 6X His tag® in Western blot and ELISA. Suitable for His-tag samples.
- Cited in over 130 publications
- Use to detect his-tagged proteins
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- Cells 12:2023Bi-Specific Killer Cell Engager Enhances NK Cell Activity against Interleukin-13 Receptor Alpha-2 Positive Gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristen D Pawlowski,Joseph T Duffy,Arushi Tiwari,Markella Zannikou,Irina V BalyasnikovaPubMed 37443750
- Nature protocols 18:2374-23982023Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica C Stark,Thapakorn Jaroentomeechai,Katherine F Warfel,Jasmine M Hershewe,Matthew P DeLisa,Michael C JewettPubMed 37328605
- Molecules (Basel, Switzerland) 28:2023Selecting Nanobodies Specific for the Epidermal Growth Factor from a Synthetic Nanobody Library.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunier Serrano-Rivero,Julieta Salazar-Uribe,Marcela Rubio-Carrasquilla,Frank Camacho-Casanova,Oliberto Sánchez-Ramos,Alaín González-Pose,Ernesto MorenoPubMed 37241784
- Molecules (Basel, Switzerland) 28:2023Design and Construction of a Synthetic Nanobody Library: Testing Its Potential with a Single Selection Round Strategy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Angélica Contreras,Yunier Serrano-Rivero,Alaín González-Pose,Julieta Salazar-Uribe,Marcela Rubio-Carrasquilla,Matheus Soares-Alves,Natalie C Parra,Frank Camacho-Casanova,Oliberto Sánchez-Ramos,Ernesto MorenoPubMed 37175117
- Biomolecules 13:2023Structural Basis of the Interaction between Human Axin2 and SIAH1 in the Wnt/β-Catenin Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLianqi Chen,Yan-Ping Liu,Li-Fei Tian,Mingzhou Li,Shuyu Yang,Song Wang,Wenqing Xu,Xiao-Xue YanPubMed 37189394
- Scientific reports 13:48912023The 8-bromobaicalein inhibited the replication of dengue, and Zika viruses and targeted the dengue polymerase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiwaporn Boonyasuppayakorn,Thanaphon Saelee,Thao Nguyen Thanh Huynh,Rita Hairani,Kowit Hengphasatporn,Naphat Loeanurit,Van Cao,Vipanee Vibulakhaophan,Panattida Siripitakpong,Parveen Kaur,Justin Jang Hann Chu,Chairat Tunghirun,Opas Choksupmanee,Sarin Chimnaronk,Yasuteru Shigeta,Thanyada Rungrotmongkol,Warinthorn ChavasiriPubMed 36966240
- Proceedings of the National Academy of Sciences of the United States of America 120:e22182381202023Structure of the Wnt-Frizzled-LRP6 initiation complex reveals the basis for coreceptor discrimination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaotaka Tsutsumi,Sunhee Hwang,Deepa Waghray,Simon Hansen,Kevin M Jude,Nan Wang,Yi Miao,Caleb R Glassman,Nathanael A Caveney,Claudia Y Janda,Rami N Hannoush,K Christopher GarciaPubMed 36893265
- ACS synthetic biology 12:95-1072022A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine F Warfel,Asher Williams,Derek A Wong,Sarah E Sobol,Primit Desai,Jie Li,Yung-Fu Chang,Matthew P DeLisa,Ashty S Karim,Michael C JewettPubMed 36548479
- Journal of virology 96:e01484222022Cross-Species Permissivity: Structure of a Goat Adeno-Associated Virus and Its Complex with the Human Receptor AAVR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdward E Large,Mark A Silveria,Onellah Weerakoon,Tommi A White,Michael S ChapmanPubMed 36453885
- The Journal of biological chemistry 299:1027322022Nanobody derived using a peptide epitope from the spike protein receptor-binding motif inhibits entry of SARS-CoV-2 variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNivya Mendon,Rayees A Ganie,Shubham Kesarwani,Drisya Dileep,Sarika Sasi,Prakash Lama,Anchal Chandra,Minhajuddin SirajuddinPubMed 36423687
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA- Form
- Liquid
- Clonality
- Polyclonal
Consider this alternative
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Tag | Expected | Tested |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Recommended products
Anti-6X His tag® HRP conjugated rabbit polyclonal antibody that is used to detect 6X His tag® in Western blot and ELISA. Suitable for His-tag samples.
- Cited in over 130 publications
- Use to detect his-tagged proteins
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- HRP
- Storage buffer
pH: 7
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
HRP Anti-6X His tag® antibody (ab1187) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, WB.
HRP Anti-6X His tag® antibody (ab1187) has been cited over 131 times in peer reviewed journals and is trusted by the scientific community.
HRP Anti-6X His tag® antibody (ab1187) has 12 independent reviews from customers.
HRP Anti-6X His tag® antibody (ab1187) specifically detects 6X His tag® (UniProt ID: P26367; Molecular weight: 47kDa) and is sold in a 100 ug selling size.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Or search our wide range of secondary antibodies for use with your experiment.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
- Biological function summary
Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.
- Pathways
The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.
- Associated diseases and disorders
The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
This image is courtesy of a customer review submitted by Mr Ohrt ThomasWestern blot - HRP Anti-6X His tag® antibody (ab1187)
All lanes: Western blot - HRP Anti-6X His tag® antibody (ab1187) at 1/4000 dilution
Lane 1: 4ug Human His-tagged protein purified from E.coli
Lane 2: 2ug Human His-tagged protein purified from E.coli
Lane 3: 1ug Human His-tagged protein purified from E.coli
Lane 4: 0.5ug Human His-tagged protein purified from E.coli
Observed band size: 136 kDa
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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