AB1187

HRP Anti-6X His tag® antibody

Name: Anti-6X His tag® HRP antibody (ab1187) Rabbit polyclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB1187
Price: 505 USD
Availability: InStock
Rating: 4 (12 reviews)

(12 Reviews)

(180 Publications)

Anti-6X His tag® antibody - HRP conjugated (ab1187) is a rabbit polyclonal antibody detecting 6X His tag® in Western Blot, ELISA.

- Over 130 publications
- Trusted since 2002

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

AbReview6871****

Western blot - HRP Anti-6X His tag® antibody (AB1187)

All lanes:

Western blot - HRP Anti-6X His tag® antibody (ab1187) at 1/4000 dilution

Lane 1:

4ug Human His-tagged protein purified from E.coli

Lane 2:

2ug Human His-tagged protein purified from E.coli

Lane 3:

1ug Human His-tagged protein purified from E.coli

Lane 4:

0.5ug Human His-tagged protein purified from E.coli

Observed band size: 136 kDa

false

This image is courtesy of an Abreview submitted by Mr Ohrt Thomas

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

ELISA, WB

applications

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

HRP Anti-6X His tag® antibody (ab1187) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, WB.

HRP Anti-6X His tag® antibody (ab1187) has been cited over 131 times in peer reviewed journals and is trusted by the scientific community.

HRP Anti-6X His tag® antibody (ab1187) has 12 independent reviews from customers.

HRP Anti-6X His tag® antibody (ab1187) specifically detects 6X His tag® (UniProt ID: P26367; Molecular weight: 47kDa) and is sold in a 100 ug selling size.

Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.

Storage buffer

pH: 7 Preservative: 0.002% Thimerosal (merthiolate) Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information 6X His tag®

style

left-column

Publications (180)

Recent publications for all applications. Explore the full list and refine your search

ACS central science 11:1670-1681 PubMed41019111

2025

Selective and Potent Peptide Binders of RNF43 for Wnt Signaling Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Sunhee Hwang,Paula Flórez Salcedo,Antonion Korcari,John M Nicoludis,Estefania Martinez Valdivia,Lingling Peng,Aaron T Balana,Justin Mak,Christopher M Crittenden,Amin Famili,Peter Liu,David Castillo-Azofeifa,Rami N Hannoush,Stephen E Miller,Christina I Schroeder,Xinxin Gao

iScience 28:113112 PubMed40792031

2025

A long-lasting prolactin stimulates galactopoiesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kasia Kready,Kailyn E Doiron,Katherine Redfield Chan,Jeffrey C Way,Quincey Justman,Camille E Powe,Pamela A Silver

Nature communications 16:7215 PubMed40764296

2025

Characterizing and engineering post-translational modifications with high-throughput cell-free expression.

Applications

Unspecified application

Species

Unspecified reactive species

Derek A Wong,Zachary M Shaver,Maria D Cabezas,Martin Daniel-Ivad,Katherine F Warfel,Deepali V Prasanna,Sarah E Sobol,Regina Fernandez,Fernando Tobias,Szymon K Filip,Sophia W Hulbert,Peter Faull,Robert Nicol,Matthew P DeLisa,Emily P Balskus,Ashty S Karim,Michael C Jewett

Nature communications 16:6152 PubMed40610439

2025

Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Belen Sotomayor,Thomas C Donahue,Sai Pooja Mahajan,May N Taw,Sophia W Hulbert,Erik J Bidstrup,D Natasha Owitipana,Alexandra Pang,Xu Yang,Souvik Ghosal,Christopher A Alabi,Parastoo Azadi,Jeffrey J Gray,Michael C Jewett,Lai-Xi Wang,Matthew P DeLisa

ACS synthetic biology 14:2354-2367 PubMed40437661

2025

Glycosylation of Structured Protein Domains in Cell-Free Reaction Environments.

Applications

Unspecified application

Species

Unspecified reactive species

Erik J Bidstrup,Kyle Hill,Chandra K Bandi,D Natasha Owitipana,Alina Chisti,Rochelle Aw,Xu Yang,Parastoo Azadi,Michael C Jewett,Lai-Xi Wang,Weston Kightlinger,Matthew P DeLisa

STAR protocols 6:103715 PubMed40158214

2025

Protocol for producing brain-derived neurotrophic factor and neurotrophin-4 in their pro and active form in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph Holzner,Katharina Böttinger,Christian G Huber,Elfriede Dall,Hans Brandstetter

The Journal of cell biology 224: PubMed40105698

2025

Structural characterization and inhibition of the interaction between ch-TOG and TACC3.

Applications

Unspecified application

Species

Unspecified reactive species

James Shelford,Selena G Burgess,Elena Rostkova,Mark W Richards,Gabrielle Larocque,Josephina Sampson,Christian Tiede,Alistair J Fielding,Tina Daviter,Darren C Tomlinson,Antonio N Calabrese,Mark Pfuhl,Richard Bayliss,Stephen J Royle

International journal of molecular sciences 26: PubMed39795995

2025

Strategic Optimization of the Middle Domain IIIA in RBP-Albumin IIIA-IB Fusion Protein to Enhance Productivity and Thermostability.

Applications

Unspecified application

Species

Unspecified reactive species

Myungho Sohn,Sanggil Kim,Hyeon Ju Jeong,In Young Ko,Ji Wook Moon,Dowon Lee,Junseo Oh

Molecular cancer therapeutics 24:406-418 PubMed39632727

2024

Preclinical Evaluation of an Anchored Immunotherapy Strategy with Aluminum Hydroxide-Tethered IL-12 in Dogs with Advanced Malignant Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Matheus Moreno Passos Barbosa,Rebecca L Kamerer,Joanna Schmit,Angel J Lopez,Rachel Uyehara,Robert Tighe,Sailaja Battula,Howard L Kaufman,Timothy M Fan

Scientific reports 14:28322 PubMed39550397

2024

Generating and validating renewable affimer protein binding reagents targeting SH2 domains.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie J Heseltine,Gregory J Billenness,Heather L Martin,Christian Tiede,Anna A S Tang,Eleanor Foy,Grace Reddy,Naomi Gibson,Matt Johnson,Michael E Webb,Michael J McPherson,Darren C Tomlinson

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-6X His tag® antibody

Western blot - HRP Anti-6X His tag® antibody (AB1187)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (180)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise