AB1269

HRP Anti-6X His tag® antibody

Name: HRP conjugated Anti-6X His tag® antibody (ab1269) | Abcam
Brand: Abcam Limited
SKU: AB1269
Price: 495 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(13 Publications)

Anti-6X His tag® antibody - HRP conjugated (ab1269) is a goat polyclonal antibody detecting 6X His tag® in Western Blot, ELISA.

- Over 10 publications
- Trusted since 2002

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

ICC, WB, ELISA

applications

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Reactivity data

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Product details

What is this antibody validated in?
HRP Anti-6X His tag® antibody (ab1269) is a goat polyclonal antibody and is validated for use in Western Blot (WB), ELISA. Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-6X His tag® HRP (ab1269) was first used in a scientific publication in 2002 and has been cited over 10 times in peer-reviewed journals.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Computational and structural biotechnology journal 21:4096-4109 PubMed37671240

2023

Human ACE2 orthologous peptide sequences show better binding affinity to SARS-CoV-2 RBD domain: Implications for drug design.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Mahmoudi Azar,Muhammed Miran Öncel,Elif Karaman,Levent Faruk Soysal,Ayesha Fatima,Sy Bing Choi,Alp Ertunga Eyupoglu,Batu Erman,Asif M Khan,Serdar Uysal

Current issues in molecular biology 45:4778-4795 PubMed37367053

2023

Large-Scale Production of Anti-RNase A VHH Expressed in Auxotrophic .

Applications

Unspecified application

Species

Unspecified reactive species

Elif Karaman,Alp Ertunga Eyüpoğlu,Lena Mahmoudi Azar,Serdar Uysal

Clinical cancer research : an official journal of the American Association for Cancer Research 29:2110-2122 PubMed37014656

2023

Collagen-Anchored Interleukin-2 and Interleukin-12 Safely Reprogram the Tumor Microenvironment in Canine Soft-Tissue Sarcomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan A Stinson,Allison Sheen,Noor Momin,Jordan Hampel,Rebecca Bernstein,Rebecca Kamerer,Bahaa Fadl-Alla,Jonathan Samuelson,Elizabeth Fink,Timothy M Fan,K Dane Wittrup

Science advances 8:eadd1170 PubMed36525496

2022

Bacterial crystalline cellulose secretion via a supramolecular BcsHD scaffold.

Applications

Unspecified application

Species

Unspecified reactive species

Wiem Abidi,Marion Decossas,Lucía Torres-Sánchez,Lucie Puygrenier,Sylvie Létoffé,Jean-Marc Ghigo,Petya V Krasteva

PLoS neglected tropical diseases 15:e0009973 PubMed34851958

2021

Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV).

Applications

Unspecified application

Species

Unspecified reactive species

Elif Karaaslan,Nesibe Selma Çetin,Merve Kalkan-Yazıcı,Sevde Hasanoğlu,Faruk Karakeçili,Aykut Özdarendeli,Ahmet Kalkan,Ali Osman Kılıç,Mehmet Ziya Doymaz

Nature communications 12:321 PubMed33436623

2021

Structural basis for the multi-activity factor Rad5 in replication stress tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Shen,Nalini Dhingra,Quan Wang,Chen Cheng,Songbiao Zhu,Xiaolin Tian,Jun Yu,Xiaoxin Gong,Xuzhichao Li,Hongwei Zhang,Xin Xu,Liting Zhai,Min Xie,Ying Gao,Haiteng Deng,Yongning He,Hengyao Niu,Xiaolan Zhao,Song Xiang

eLife 9: PubMed32459174

2020

Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xu,Huigang Shi,Xiaowen Gong,Pu Chen,Ying Gao,Xinzheng Zhang,Song Xiang

Nature immunology 18:889-898 PubMed28604720

2017

IgG Fc domains that bind C1q but not effector Fcγ receptors delineate the importance of complement-mediated effector functions.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Han Lee,Gabrielle Romain,Wupeng Yan,Makiko Watanabe,Wissam Charab,Biliana Todorova,Jiwon Lee,Kendra Triplett,Moses Donkor,Oana I Lungu,Anja Lux,Nicholas Marshall,Margaret A Lindorfer,Odile Richard-Le Goff,Bianca Balbino,Tae Hyun Kang,Hidetaka Tanno,George Delidakis,Corrine Alford,Ronald P Taylor,Falk Nimmerjahn,Navin Varadarajan,Pierre Bruhns,Yan Jessie Zhang,George Georgiou

Nature communications 7:12137 PubMed27378283

2016

Engineering biosynthesis of the anticancer alkaloid noscapine in yeast.

Applications

Species

Unspecified reactive species

Yanran Li,Christina D Smolke

PloS one 8:e81350 PubMed24260574

2013

Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP.

Applications

ELISA

Species

Unspecified reactive species

Johan Seijsing,Malin Lindborg,John Löfblom,Mathias Uhlén,Torbjörn Gräslund

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-6X His tag® antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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