AB178563

HRP Anti-6X His tag® antibody [AD1.1.10]

Name: HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563) | Abcam
Brand: Abcam Limited
SKU: AB178563
Price: 420 USD
Availability: InStock

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(3 Publications)

Anti-6X His tag® antibody [AD1.1.10] - HRP conjugated (ab178563) is a mouse monoclonal antibody detecting 6X His tag® in Western Blot, ELISA.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Supplier Data

Western blot - HRP Anti-6X His tag® antibody [AD1.1.10] (AB178563)

Western Blot of a 41kDa protein fused (expressed in E.Coli) to His tag using ab178563 0.25 μg/ml. Loading was confirmed by Coomassi blue staining of transferred proteins.

All lanes:

Western blot - HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)

false

673 DyLight® 650

DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]
Unconjugated

Anti-6X His tag® antibody [AD1.1.10]
Alkaline Phosphatase

Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10]
518 DyLight® 488

DyLight® 488 Anti-6X His tag® antibody [AD1.1.10]
576 DyLight® 550

DyLight® 550 Anti-6X His tag® antibody [AD1.1.10]
578 PE

PE Anti-6X His tag® antibody [AD1.1.10]
675 PerCP

PerCP Anti-6X His tag® antibody [AD1.1.10]
657 SureLight® APC

SureLight® APC Anti-6X His tag® antibody [AD1.1.10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD1.1.10

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

WB, ELISA

applications

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Reactivity data

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Product details

What is this antibody validated in?
HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), ELISA. Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone AD1.1.10 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® HRP [AD1.1.10] (ab178563).

Other related products
We have a range of other formats of antibody clone [AD1.1.10] also available for your convenience: ab15149, PE - ab72467, SureLight® APC - ab72579, Alkaline Phosphatase - ab81652, PerCP - ab117496, Carrier free - ab117504, DyLight® 550 - ab117507, DyLight® 488 - ab117512, HRP - ab178563, Carrier free - ab307236

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute with 1.0 ml distilled deionized water. Vortex gently, sit on ice for 20 min. Final concentration: 0.1 mg/ml. Determine optimal working concentration per application. Avoid heat/light. Lyophilized: stable 1 year. After reconstitution: store at 2–8 °C in dark, use within 6 months. If further diluted, use within 1 week.

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.71% Sucrose, 1.5% BSA, 1.4% Sodium phosphate, 0.58% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced materials (Deerfield Beach, Fla.) 35:e2300575 PubMed36932971

2023

Artificial Lipid Biomembranes for Full-Length SARS-CoV-2 Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Wang,Xiaomei Lin,Yuting Li,Yuan Lu

Theranostics 12:7729-7744 PubMed36451853

2022

Self-assembly of X-shaped antibody to combine the activity of IgG and IgA for enhanced tumor killing.

Applications

Unspecified application

Species

Unspecified reactive species

Yuexia Liang,Xin Li,Fengping Peng,Xiaohan Ye,Wei Wang,Tianyi Cen,Fan Li,Yue Lu,Zhaoyun Liu,Hui Liu,Kai Ding,Kai Ye,Yang Yu,Tianyu Ma,Sihe Zhang,Yi Huang,Yuan Wang,Xue Yang,Rong Fu,Hongkai Zhang

European journal of pharmaceutical sciences : offi 122:42-50 PubMed29933078

2018

Multivalent targeting and killing of HER2 overexpressing breast carcinoma cells with methotrexate-encapsulated tetra-specific non-overlapping variable domain heavy chain anti-HER2 antibody-PEG-liposomes: In vitro proof-of-concept.

Applications

Unspecified application

Species

Unspecified reactive species

Shahryar Khoshtinat Nikkhoi,Fatemeh Rahbarizadeh,Davoud Ahmadvand,Seyed Moein Moghimi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-6X His tag® antibody [AD1.1.10]

Western blot - HRP Anti-6X His tag® antibody [AD1.1.10] (AB178563)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

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Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise