AB237339

HRP Anti-6X His tag® antibody [EPR20547]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal 6X His tag® antibody - conjugated to HRP. Suitable for WB and reacts with Tag samples. Cited in 5 publications.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

2 Images

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Western blot - HRP Anti-6X His tag® antibody [EPR20547] (AB237339)

All lanes:

Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339) at 1/50000 dilution

Lane 1:

HEK-293 transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 15 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag at 15 µg

Observed band size: 125 kDa

false

Exposure time: 3min

Lab

Western blot - HRP Anti-6X His tag® antibody [EPR20547] (AB237339)

ab237339 was shown to react with 6X His tag^® in western blot. Membranes were blocked in 5% milk in TBS-T (0.1% Tween^®) before incubation with ab237339 overnight at 4°C at a 1 in 5000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.

All lanes:

Western blot - HRP Anti-6X His tag® antibody [EPR20547] (ab237339) at 1/5000 dilution

Lane 1:

Histone H3 recombinant (<a href='/en-us/products/proteins-peptides/recombinant-human-histone-h3-protein-active-ab198757'>ab198757</a>) (4 minute exposure) cell lysate at 2 µg

Lane 2:

Histone H4 recombinant (<a href='/en-us/products/proteins-peptides/recombinant-human-histone-h4-protein-ab198115'>ab198115</a>) (1 minute 30 seconds exposure) cell lysate at 2 µg

Lane 3:

Histone H2A recombinant (<a href='/en-us/products/proteins-peptides/recombinant-human-histone-h2a-protein-ab200295'>ab200295</a>)(1 minute 30 seconds exposure) cell lysate at 2 µg

Observed band size: 17 kDa

false

578 PE

PE Anti-6X His tag® antibody [EPR20547]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]
Biotin

Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade
660 APC

APC Anti-6X His tag® antibody [EPR20547] - ChIP Grade
519 FITC

FITC Anti-6X His tag® [EPR20547]
Unconjugated

Anti-6X His tag® antibody [EPR20547] - ChIP Grade
Carrier free

Anti-6X His tag® antibody [EPR20547] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20547

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1322 PubMed40885856

2025

Engineering virus-like particles for safe and versatile modeling of SARS-CoV-2 host interaction and immune escape.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Ma,Yuan Lin,Siyu Ma,Kai Chen,Yihan Lin

Molecular therapy. Nucleic acids 35:102304 PubMed39281707

2024

Nanobody against SARS-CoV-2 non-structural protein Nsp9 inhibits viral replication in human airway epithelia.

Applications

Unspecified application

Species

Unspecified reactive species

Tomas Venit,Jeremy Blavier,Sibusiso B Maseko,Sam Shu,Lilia Espada,Christopher Breunig,Hans-Peter Holthoff,Sabrina C Desbordes,Martin Lohse,Gennaro Esposito,Jean-Claude Twizere,Piergiorgio Percipalle

Molecular therapy : the journal of the American Society of Gene Therapy 32:2676-2691 PubMed38959896

2024

Human plasma cells engineered to secrete bispecifics drive effective in vivo leukemia killing.

Applications

Unspecified application

Species

Unspecified reactive species

Tyler F Hill,Parnal Narvekar,Gregory D Asher,Jasmine N Edelstein,Nathan D Camp,Annaiz Grimm,Kerri R Thomas,Michael D Leiken,Katherine M Molloy,Peter J Cook,Sean P Arlauckas,Richard A Morgan,Sarah K Tasian,David J Rawlings,Richard G James

Nature communications 14:4436 PubMed37481670

2023

LILRB2/PirB mediates macrophage recruitment in fibrogenesis of nonalcoholic steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Pei Li,Li Huang,Ran-Ran Kan,Xiao-Yu Meng,Shu-Yun Wang,Hua-Jie Zou,Ya-Ming Guo,Pei-Qiong Luo,Li-Meng Pan,Yu-Xi Xiang,Bei-Bei Mao,Yu-Yu Xie,Zhi-Han Wang,Min Yang,Rui He,Yan Yang,Zhe-Long Liu,Jun-Hui Xie,De-Lin Ma,Ben-Ping Zhang,Shi-Ying Shao,Xi Chen,Si-Miao Xu,Wen-Tao He,Wen-Jun Li,Yong Chen,Xue-Feng Yu

Development, growth & differentiation 64:88-97 PubMed34519039

2021

A small Rho GTPase OsRacB is required for pollen germination in rice.

Applications

Unspecified application

Species

Unspecified reactive species

Yangfan Xu,Wenguo Cai,Xiaofei Chen,Mingjiao Chen,Wanqi Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-6X His tag® antibody [EPR20547]

Western blot - HRP Anti-6X His tag® antibody [EPR20547] (AB237339)

Western blot - HRP Anti-6X His tag® antibody [EPR20547] (AB237339)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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