Rabbit Recombinant Monoclonal ABCE1 antibody - conjugated to HRP. C-terminal. Suitable for WB and reacts with Human samples.
Images
![Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB209043), expandable thumbnail](https://content.abcam.com/products/images/hrp-abce1-antibody-epr15373b-c-terminal-ab209043--western-blot-img94517.jpg/_jcr_content/renditions/webp-475.webp "Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB209043)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway (PubMed:21448132). Together with PELO and HBS1L, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes (PubMed:21448132). Together with PELO and HBS1L, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway (PubMed:21448132). Plays a role in the regulation of mRNA turnover (By similarity). Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (PubMed:29861391). As part of the PINK1-regulated signaling, ubiquitinated by CNOT4 upon mitochondria damage; this modification generates polyubiquitin signals that recruit autophagy receptors to the mitochondrial outer membrane and initiate mitophagy (PubMed:29861391). RNASEL-specific protein inhibitor which antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) to RNASEL (PubMed:9660177). Negative regulator of the anti-viral effect of the interferon-regulated 2-5A/RNASEL pathway (PubMed:9660177, PubMed:9847332, PubMed:11585831).(Microbial infection) May act as a chaperone for post-translational events during HIV-1 capsid assembly.(Microbial infection) Plays a role in the down-regulation of the 2-5A/RNASEL pathway during encephalomyocarditis virus (EMCV) and HIV-1 infections.
Alternative names
ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein, HuHP68, RNase L inhibitor, Ribonuclease 4 inhibitor, RNS4I, ABCE1, RLI, RNS4I, RNASEL1, OK/SW-cl.40, RNASELI
Recommended products
Rabbit Recombinant Monoclonal ABCE1 antibody - conjugated to HRP. C-terminal. Suitable for WB and reacts with Human samples.
Alternative names
ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein, HuHP68, RNase L inhibitor, Ribonuclease 4 inhibitor, RNS4I, ABCE1, RLI, RNS4I, RNASEL1, OK/SW-cl.40, RNASELI
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
HRP
- Storage buffer
pH: 7.4
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR15373(B)
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Stable for 12 months at -20°C, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ABCE1 also known as ATP-binding cassette sub-family E member 1 is an ATPase involved in various cellular processes. It belongs to the ATP-binding cassette (ABC) transporter family. The protein mass is approximately 68 kDa and its expression occurs widely in various tissues including the cytoplasm and mitochondria. ABCE1 has a unique structure with two nucleotide-binding domains making it distinct from other ABC family members that typically transport molecules across membranes.
- Biological function summary
ABCE1 plays a role in inhibiting ribonuclease activity and is essential in ribosome recycling. This protein interacts with translation initiation factors and actively participates in the dissociation of ribosomes after translation termination. It is a part of the ribosome recycling complex an important multi-proteins setup necessary to prepare ribosomes for another round of translation. The biochemical interactions and presence in different cellular compartments highlight its multiple roles in cellular homeostasis.
- Pathways
ABCE1 engages significantly in translation and its regulation. It plays an important role in the translation termination pathway where it interacts with eukaryotic release factors. Additionally it influences the degradation pathway of defective ribosomal products. Within these pathways the function of ABCE1 is closely linked to proteins like eRF1 and eRF3 which are essential in the translation termination process. The interaction of these proteins ensures efficient termination and recycling of translation machinery components.
- Associated diseases and disorders
Alterations in ABCE1 expression or function link to certain cancers and viral infections. The aberrant activity of ABCE1 influences cancer progression as it affects protein synthesis and cellular growth levels. Also ABCE1 has been implicated in promoting the replication of certain viruses providing them a competitive advantage. Proteins like eIF3 and eIF4 involved in translation initiation can show altered associations in disorders where ABCE1 is dysregulated influencing disease outcomes and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043), expandable thumbnail](https://content.abcam.com/products/images/hrp-abce1-antibody-epr15373b-c-terminal-ab209043--western-blot-img94517.jpg "Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)")
Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209043 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043) at 1/5000 dilution
Lane 1: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 2: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg
Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 2min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com