AB209043

HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ABCE1 antibody - conjugated to HRP. C-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

RLI, RNASEL1, RNASELI, RNS4I, OK/SW-cl.40, ABCE1, ATP-binding cassette sub-family E member 1, 2'-5'-oligoadenylate-binding protein, HuHP68, RNase L inhibitor, Ribonuclease 4 inhibitor

1 Images

Lab

Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB209043)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209043 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab209043) at 1/5000 dilution

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 2:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 67 kDa

Observed band size: 67 kDa

true

Exposure time: 2min

578 PE

PE Anti-ABCE1 antibody [EPR15373(B)] - C-terminal
Unconjugated

Anti-ABCE1 antibody [EPR15373(B)] - C-terminal
Carrier free

Anti-ABCE1 antibody [EPR15373(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15373(B)

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCE1 also known as ATP-binding cassette sub-family E member 1 is an ATPase involved in various cellular processes. It belongs to the ATP-binding cassette (ABC) transporter family. The protein mass is approximately 68 kDa and its expression occurs widely in various tissues including the cytoplasm and mitochondria. ABCE1 has a unique structure with two nucleotide-binding domains making it distinct from other ABC family members that typically transport molecules across membranes.

Biological function summary

ABCE1 plays a role in inhibiting ribonuclease activity and is essential in ribosome recycling. This protein interacts with translation initiation factors and actively participates in the dissociation of ribosomes after translation termination. It is a part of the ribosome recycling complex an important multi-proteins setup necessary to prepare ribosomes for another round of translation. The biochemical interactions and presence in different cellular compartments highlight its multiple roles in cellular homeostasis.

Pathways

ABCE1 engages significantly in translation and its regulation. It plays an important role in the translation termination pathway where it interacts with eukaryotic release factors. Additionally it influences the degradation pathway of defective ribosomal products. Within these pathways the function of ABCE1 is closely linked to proteins like eRF1 and eRF3 which are essential in the translation termination process. The interaction of these proteins ensures efficient termination and recycling of translation machinery components.

Alterations in ABCE1 expression or function link to certain cancers and viral infections. The aberrant activity of ABCE1 influences cancer progression as it affects protein synthesis and cellular growth levels. Also ABCE1 has been implicated in promoting the replication of certain viruses providing them a competitive advantage. Proteins like eIF3 and eIF4 involved in translation initiation can show altered associations in disorders where ABCE1 is dysregulated influencing disease outcomes and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nucleoside-triphosphatase (NTPase) involved in ribosome recycling by mediating ribosome disassembly (PubMed : 20122402, PubMed : 21448132). Able to hydrolyze ATP, GTP, UTP and CTP (PubMed : 20122402). Splits ribosomes into free 60S subunits and tRNA- and mRNA-bound 40S subunits (PubMed : 20122402, PubMed : 21448132). Acts either after canonical termination facilitated by release factors (ETF1/eRF1) or after recognition of stalled and vacant ribosomes by mRNA surveillance factors (PELO/Pelota) (PubMed : 20122402, PubMed : 21448132). Involved in the No-Go Decay (NGD) pathway : recruited to stalled ribosomes by the Pelota-HBS1L complex, and drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed : 21448132). Also plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA (PubMed : 29861391). As part of the PINK1-regulated signaling, ubiquitinated by CNOT4 upon mitochondria damage; this modification generates polyubiquitin signals that recruit autophagy receptors to the mitochondrial outer membrane and initiate mitophagy (PubMed : 29861391). RNASEL-specific protein inhibitor which antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) to RNASEL (PubMed : 9660177). Negative regulator of the anti-viral effect of the interferon-regulated 2-5A/RNASEL pathway (PubMed : 11585831, PubMed : 9660177, PubMed : 9847332).. (Microbial infection) May act as a chaperone for post-translational events during HIV-1 capsid assembly.. (Microbial infection) Plays a role in the down-regulation of the 2-5A/RNASEL pathway during encephalomyocarditis virus (EMCV) and HIV-1 infections.

See full target information ABCE1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33300072

2020

miR‑654‑3p suppresses cell viability and promotes apoptosis by targeting RASAL2 in non‑small‑cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xiong,Shigang Xing,Zheng Dong,Lei Niu,Qinghua Xu,Yusheng Li,Pingyi Liu,Peixia Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal

Western blot - HRP Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB209043)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise