AB201776

HRP Anti-ABCG1 antibody [EP1366Y]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal ABCG1 antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

ABC8, WHT1, ABCG1, ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, White protein homolog

1 Images

Lab

Western blot - HRP Anti-ABCG1 antibody [EP1366Y] (AB201776)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201776 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ABCG1 antibody [EP1366Y] (ab201776) at 1/5000 dilution

All lanes:

HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg

Predicted band size: 75 kDa

Observed band size: 110 kDa

true

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1366Y

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ABCG1 also known as ATP-binding cassette sub-family G member 1 functions as a cholesterol transporter in cells. The protein facilitates the efflux of cholesterol to high-density lipoproteins (HDL). With a molecular mass of approximately 75 kDa ABCG1 is located in many tissues but its expression levels are highest in macrophages and endothelial cells. As a member of the ABC transporter family it plays a critical role in cellular lipid homeostasis.

Biological function summary

ABCG1 works to regulate cholesterol levels by exporting excess cholesterol and phospholipids. This protein does not operate in isolation; it often interacts with other proteins forming heterodimers especially with ABCG4. These interactions contribute to its role in maintaining lipid metabolism by facilitating efficient cholesterol clearance improving cellular lipid balance and reducing intracellular lipid toxicity.

Pathways

ABCG1 plays an important role in the reverse cholesterol transport pathway and is involved in lipid metabolism. In the context of these pathways ABCG1 interacts with proteins like LXR (liver X receptor) which regulates its expression. LXRs when activated by oxysterols can influence ABCG1 activity promoting cholesterol efflux to HDL and contributing to overall cholesterol homeostasis in the body.

ABCG1 has connections to atherosclerosis and metabolic syndrome. Disruption in ABCG1 function or expression can lead to impaired cholesterol efflux contributing to lipid accumulation in macrophages and formation of foam cells an important event in atherosclerosis development. In this disease context ABCG1 interacts with proteins like ApoA-I and ApoA-II which are components of HDL and are essential for efficient cholesterol transport to the liver highlighting its critical role in cardiovascular health management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP (PubMed : 17408620, PubMed : 24576892). The lipid efflux is ALB-dependent (PubMed : 16702602). Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol (PubMed : 17408620).

See full target information ABCG1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 24: PubMed34278466

2021

Chemokine CCL20 promotes the paclitaxel resistance of CD44CD117 cells via the Notch1 signaling pathway in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Min Chen,Juan Su,Chunmei Feng,Ying Liu,Li Zhao,Yongjie Tian

Drug design, development and therapy 14:731-744 PubMed32158192

2020

Ox-LDL Causes Endothelial Cell Injury Through ASK1/NLRP3-Mediated Inflammasome Activation via Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Hang,Yan Peng,Rui Xiang,Xiangdong Li,Zhiliang Li

Journal of Cancer 9:556-563 PubMed29483961

2018

The prognostic value of Niemann-Pick C1-like protein 1 and Niemann-Pick disease type C2 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ke-Ji Chen,Ri-Ming Jin,Chun-Chao Shi,Rui-Liang Ge,Lei Hu,Qi-Fei Zou,Quan-Yu Cai,Guang-Zhi Jin,Kui Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-ABCG1 antibody [EP1366Y]

Western blot - HRP Anti-ABCG1 antibody [EP1366Y] (AB201776)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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