AB197716

HRP Anti-ACTC1 antibody [EP184E]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ACTC antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ACTC, ACTC1, Alpha-cardiac actin

1 Images

Lab

Western blot - HRP Anti-ACTC1 antibody [EP184E] (AB197716)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197716 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ACTC1 antibody [EP184E] (ab197716) at 1/5000 dilution

All lanes:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 42 kDa

Observed band size: 42 kDa

true

Exposure time: 20min

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ACTC1 antibody [EP184E]
Unconjugated

Anti-ACTC1 antibody [EP184E]
Carrier free

Anti-ACTC1 antibody [EP184E] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP184E

Isotype

IgG

Conjugation

HRP

Excitation/Emission

Carrier free

Reacts with

Human, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTC1 also known as cardiac alpha actin is a protein that plays an essential role in the cytoskeletal structure of heart muscle cells. Weighing around 42 kDa ACTC1 is found predominantly in cardiac tissue specifically in the sarcomere which is the fundamental unit of muscle contraction. In muscle cells ACTC1 organizes into thin filaments and is critical for maintaining the structural integrity and contractile function of the cardiac tissue.

Biological function summary

ACTC1 is significant in muscle contraction and cellular movement and it forms part of the sarcomeric complex within cardiac muscle cells. This protein along with other actin isoforms contributes to the organized network of actin and myosin filaments which generate force for heartbeats. ACTC1 polymerizes to form the actin filament which when coupled with myosin motors and regulatory proteins executes the contraction cycle in cardiac muscles.

Pathways

ACTC1 plays a central role within the muscle contraction process and the cardiac muscle contraction pathway. In these pathways its interaction with proteins like myosin tropomyosin and troponin is vital to initiate and regulate cardiac contractions. The dynamic interaction between ACTC1 and these proteins facilitates the conversion of chemical energy into mechanical energy necessary for the rhythmic contraction and relaxation of heart muscles.

Mutations or alterations in ACTC1 are closely linked to cardiac conditions such as hypertrophic cardiomyopathy and dilated cardiomyopathy. These disorders often involve associated proteins like troponin T and myosin heavy chain which when mutated along with ACTC1 disrupt normal cardiac contraction and lead to structural heart changes. These conditions highlight the importance of ACTC1 in maintaining normal cardiac function and structure.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTC1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 151: PubMed39431301

2024

The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre Simonin,Gehenna Lobo Guerrero,Sabine Bardin,Ram Venkata Gannavarapu,Denis Krndija,Joseph Boyd,Stephanie Miserey,Danijela Matic Vignjevic,Bruno Goud

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

HRP Anti-ACTC1 antibody [EP184E]

Western blot - HRP Anti-ACTC1 antibody [EP184E] (AB197716)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise