JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB200774

HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Aldolase antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

ALDA, ALDOA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDC, ALDOC, Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

2 Images
Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200774)
  • WB

Lab

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200774)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab200774 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774) at 1/5000 dilution

Lane 1:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Lane 2:

Fetal Liver (Human) Normal Tissue) at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Fetal Brain (Human) Normal Tissue lysate at 10 µg

Lane 5:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Observed band size: 39 kDa

true

Exposure time: 4min

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200774)
  • WB

Unknown

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200774)

Lanes 1 - 3:

Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab169544'>ab169544</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200774) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/alexa-fluor-488-aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab200771'>ab200771</a>) at 1/1000 dilution

Lane 1:

His-tagged human Aldolase A full-length recombinant protein at 0.01 µg

Lane 2:

His-tagged human Aldolase B full-length recombinant protein at 0.01 µg

Lane 3:

His-tagged human Aldolase C full-length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

  • Unconjugated

    Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

  • Carrier free

    Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9724(B)

Isotype

IgG

Conjugation

HRP

Excitation/Emission
Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Reagents, Controls & Accessories

AB269260

ALDOA positive control ChIP primer pair

ChIP - ALDOA positive control ChIP primer pair (AB269260)

  • 0
  • 0
Primary Antibodies

AB245469

Anti-Aldolase antibody

Immunoprecipitation - Anti-Aldolase antibody (AB245469)

  • 0
  • 0
Primary Antibodies

AB150396

Anti-Aldolase antibody [EPR9725]

RabMAb|Recombinant

Western blot - Anti-Aldolase antibody [EPR9725] (AB150396)

  • 0
  • 0
Primary Antibodies

AB181662

HRP Anti-Aldolase antibody

Western blot - HRP Anti-Aldolase antibody (AB181662)

  • 0
  • 0

Complementary Products

Assay Kits

AB196994

Aldolase Activity Assay Kit (Colorimetric)

Functional Studies - Aldolase Activity Assay Kit (Colorimetric) (AB196994)

  • 0
  • 0
Proteins & Peptides

AB123165

Recombinant Human Aldolase B protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Aldolase B protein (His tag N-Terminus) (AB123165)

  • 0
  • 0
Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

  • 0
  • 0
Proteins & Peptides

AB101710

Recombinant human TSSK1 protein

Functional Studies - Recombinant human TSSK1 protein (AB101710)

  • 0
  • 0
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase which includes the isozymes Aldolase A Aldolase B and Aldolase C serves an important role by cleaving fructose 16-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate during glycolysis. This enzymatic activity is critical for cellular energy production. Aldolase A with a molecular weight of approximately 39 kDa is mainly expressed in muscle and red blood cells. Aldolase B is found in the liver and kidney while Aldolase C predominates in the brain and nervous tissue. These isozymes have similar catalytic functions but tissue-specific expression and regulation differ among them.
Biological function summary

The aldolase enzymes participate in glycolytic and gluconeogenic pathways performing critical roles in sugar metabolism. They do not usually form part of larger complexes but function alongside other metabolic enzymes in a coordinated manner. Aldolase A B and C adapt to the specific energy needs of the tissues where they are expressed contributing to cellular metabolism's adaptability. This specialization allows tissues to manage their energy production relative to their specific physiological requirements.

Pathways

Enzymes like aldolase play an important role in the glycolysis and fructose metabolism pathways. These pathways are essential for energy production and metabolic balance. During glycolysis aldolase works closely with other enzymes such as hexokinase and phosphofructokinase to efficiently convert glucose into pyruvate. In fructose metabolism in the liver Aldolase B helps in processing dietary fructose into intermediates that enter glycolysis therefore connecting fructose and glucose metabolism in liver cells.

Disturbances in aldolase function associate with conditions like hereditary fructose intolerance and hemolytic anemia. Hereditary fructose intolerance results from mutations in the Aldolase B gene leading to an inability to metabolize fructose properly. Hemolytic anemia can link to overactive glycolysis often connected with increased Aldolase A activity. These conditions illustrate how improper expression or regulation of aldolase enzymes can cause metabolic imbalances leading to serious health implications.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed : 14766013). In addition, may also function as scaffolding protein (By similarity).
See full target information ALDOA

Additional targets

ALDOB,ALDOC
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 131: PubMed30154209

2018

T cell activation triggers reversible inosine-5'-monophosphate dehydrogenase assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Krisna C Duong-Ly,Yin-Ming Kuo,Matthew C Johnson,Joy M Cote,Justin M Kollman,Jonathan Soboloff,Glenn F Rall,Andrew J Andrews,Jeffrey R Peterson
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com